1887

Abstract

A significant number of strains from pristine waters of two brooks in Finland formed a distinct cluster on the basis of whole-cell protein fingerprinting by one-dimensional SDS-PAGE. The strains shared the following characteristics. Cells were ovoid, Gram-positive-staining and non-spore-forming, appearing singly or in pairs or chains. They were facultatively anaerobic and catalase-negative. Growth in broth containing 6.5 % NaCl or at 45 °C was weak or absent. Production of D antigen was variable. The strains tolerated 60 °C for 30 min, 40 % bile and tellurite, hydrolysed aesculin strongly and gelatin weakly, produced no acid from hippurate and did not reduce it, grew weakly at 10 °C, showed a strong reaction for the Voges–Proskauer test and produced acid from methyl α--glucoside, mannitol, sorbitol and sucrose, with weak or no production of acid from methyl α--mannoside, -arabinose, gluconate and -xylose. Several of the strains were selected for identification on the basis of sequencing of almost the whole 16S rRNA gene and partial and genes and of (GTG)-PCR fingerprints. Partial and gene sequencing was also performed for those type strains of species without available sequences in the database. The pristine brook isolates formed a novel species, for which the name sp. nov. (type strain S299  = HAMBI 3055  = LMG 25899  = CCM 7986) is proposed. On the basis of 16S rRNA gene sequence similarity, sp. nov. is related to the genogoup. It is distinguished from described species on the basis of 16S rRNA, and gene sequences and whole-cell protein and (GTG)-PCR fingerprints. It is most closely related to , but DNA–DNA hybridization confirms it to represent a novel species.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.038257-0
2012-09-01
2019-10-22
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/9/2169.html?itemId=/content/journal/ijsem/10.1099/ijs.0.038257-0&mimeType=html&fmt=ahah

References

  1. Carvalho M. G., Shewmaker P. L., Steigerwalt A. G., Morey R. E., Sampson A. J., Joyce K., Barrett T. J., Teixeira L. M., Facklam R. R.. ( 2006;). Enterococcus caccae sp. nov., isolated from human stools. . Int J Syst Evol Microbiol 56:, 1505–1508. [CrossRef][PubMed]
    [Google Scholar]
  2. Clausen E. M., Green B. L., Litsky W.. ( 1977;). Fecal streptococci: indicators of pollution. . In Bacterial Indicators/Health Hazards Associated with Water (ASTM Publication 635), pp. 247–264. Edited by Hoadley A. W., Dutka B. J.. Philadelphia:: American Society for Testing and Materials;. [CrossRef]
    [Google Scholar]
  3. Cole J. R., Wang Q., Cardenas E., Fish J., Chai B., Farris R. J., Kulam-Syed-Mohideen A. S., McGarrell D. M., Marsh T.. & other authors ( 2009;). The Ribosomal Database Project: improved alignments and new tools for rRNA analysis. . Nucleic Acids Res 37:, D141–D145. [CrossRef][PubMed]
    [Google Scholar]
  4. Edwards U., Rogall T., Blöcker H., Emde M., Böttger E. C.. ( 1989;). Isolation and direct complete nucleotide determination of entire genes. Characterization of a gene coding for 16S ribosomal RNA. . Nucleic Acids Res 17:, 7843–7853. [CrossRef][PubMed]
    [Google Scholar]
  5. Ezaki T., Hashimoto Y., Yabuuchi E.. ( 1989;). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39:, 224–229. [CrossRef]
    [Google Scholar]
  6. Facklam R., Wilkinson W.. ( 1981;). The family Streptococcaceae (medical aspects). . In The Prokaryotes, pp. 1572–1597. Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. G... Berlin:: Springer;.
    [Google Scholar]
  7. Goris J., Suzuki K., De Vos P., Nakase T., Kersters K.. ( 1998;). Evaluation of a microplate DNA-DNA hybridization method compared with the initial renaturation method. . Can J Microbiol 44:, 1148–1153. [CrossRef]
    [Google Scholar]
  8. Katoh K., Toh H.. ( 2008;). Recent developments in the MAFFT multiple sequence alignment program. . Brief Bioinform 9:, 286–298. [CrossRef][PubMed]
    [Google Scholar]
  9. Naser S., Thompson F. L., Hoste B., Gevers D., Vandemeulebroecke K., Cleenwerck I., Thompson C. C., Vancanneyt M., Swings J.. ( 2005a;). Phylogeny and identification of enterococci by atpA gene sequence analysis. . J Clin Microbiol 43:, 2224–2230. [CrossRef][PubMed]
    [Google Scholar]
  10. Naser S. M., Thompson F. L., Hoste B., Gevers D., Dawyndt P., Vancanneyt M., Swings J.. ( 2005b;). Application of multilocus sequence analysis (MLSA) for rapid identification of Enterococcus species based on rpoA and pheS genes. . Microbiology 151:, 2141–2150. [CrossRef][PubMed]
    [Google Scholar]
  11. Niemi R. M., Niemelä S. I., Bamford D. H., Hantula J., Hyvärinen T., Forsten T., Raateland A.. ( 1993;). Presumptive fecal streptococci in environmental samples characterized by one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis. . Appl Environ Microbiol 59:, 2190–2196.[PubMed]
    [Google Scholar]
  12. Pot B., Vandamme P., Kersters K.. ( 1994;). Analysis of electrophoretic whole-organism protein fingerprints. . In Modern Microbiological Methods: Chemical Methods in Prokaryotic Systematics, pp. 493–521. Edited by Goodfellow M., O’Donnell A. G... Chichester:: Wiley;.
    [Google Scholar]
  13. Schleifer K. H., Kilpper-Bälz R.. ( 1984;). Transfer of Streptococcus faecalis and Streptococcus faecium to the genus Enterococcus nom. rev. as Enterococcus faecalis comb. nov. and Enterococcus faecium comb. nov.. Int J Syst Bacteriol 34:, 31–34. [CrossRef]
    [Google Scholar]
  14. Sistek V., Maheux A. F., Boissinot M., Bernard K. A., Cantin P., Cleenwerck I., De Vos P., Bergeron M. G.. ( 2012;). Enterococcus ureasiticus sp. nov. and Enterococcus quebecensis sp. nov., isolated from water. . Int J Syst Evol Microbiol 62:, 1314–1320. [CrossRef][PubMed]
    [Google Scholar]
  15. Staden R., Beal K. F., Bonfield J. K.. ( 2000;). The Staden package, 1998. . Methods Mol Biol 132:, 115–130.[PubMed]
    [Google Scholar]
  16. Stamatakis A.. ( 2006;). RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. . Bioinformatics 22:, 2688–2690. [CrossRef][PubMed]
    [Google Scholar]
  17. Švec P., Devriese L. A.. ( 2009;). Genus I. Enterococcus (ex Thiercelin and Jouhaud 1903) Schleifer and Kilpper-Bälz 1984, 32VP. . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 3, pp. 594–607. Edited by De Vos P., Garrity G. M., Jones D., Krieg N. R., Ludwig W., Rainey F. A., Schleifer K. H., Whitman W. B... New York:: Springer;.
    [Google Scholar]
  18. Švec P., Devriese L. A., Sedláček I., Baele M., Vancanneyt M., Haesebrouck F., Swings J., Doškař J.. ( 2001;). Enterococcus haemoperoxidus sp. nov. and Enterococcus moraviensis sp. nov., isolated from water. . Int J Syst Evol Microbiol 51:, 1567–1574.[PubMed]
    [Google Scholar]
  19. Švec P., Vancanneyt M., Devriese L. A., Naser S. M., Snauwaert C., Lefebvre K., Hoste B., Swings J.. ( 2005;). Enterococcus aquimarinus sp. nov., isolated from sea water. . Int J Syst Evol Microbiol 55:, 2183–2187. [CrossRef][PubMed]
    [Google Scholar]
  20. Švec P., Vancanneyt M., Sedláček I., Naser S. M., Snauwaert C., Lefebvre K., Hoste B., Swings J.. ( 2006;). Enterococcus silesiacus sp. nov. and Enterococcus termitis sp. nov.. Int J Syst Evol Microbiol 56:, 577–581. [CrossRef][PubMed]
    [Google Scholar]
  21. Švec P., Nováková D., Zácková L., Kukletová M., Sedláček I.. ( 2008;). Evaluation of (GTG)5-PCR for rapid identification of Streptococcus mutans. . Antonie van Leeuwenhoek 94:, 573–579. [CrossRef][PubMed]
    [Google Scholar]
  22. Švec P., Vandamme P., Bryndová H., Holochová P., Kosina M., Mašlaňová I., Sedláček I.. ( 2012;). Enterococcus plantarum sp. nov., isolated from plants. . Int J Syst Evol Microbiol 62:, 1499–1505. [CrossRef][PubMed]
    [Google Scholar]
  23. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J.. ( 1991;). 16S ribosomal DNA amplification for phylogenetic study. . J Bacteriol 173:, 697–703.[PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.038257-0
Loading
/content/journal/ijsem/10.1099/ijs.0.038257-0
Loading

Data & Media loading...

Supplements

Supplementary Material 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error