1887

Abstract

A significant number of strains from pristine waters of two brooks in Finland formed a distinct cluster on the basis of whole-cell protein fingerprinting by one-dimensional SDS-PAGE. The strains shared the following characteristics. Cells were ovoid, Gram-positive-staining and non-spore-forming, appearing singly or in pairs or chains. They were facultatively anaerobic and catalase-negative. Growth in broth containing 6.5 % NaCl or at 45 °C was weak or absent. Production of D antigen was variable. The strains tolerated 60 °C for 30 min, 40 % bile and tellurite, hydrolysed aesculin strongly and gelatin weakly, produced no acid from hippurate and did not reduce it, grew weakly at 10 °C, showed a strong reaction for the Voges–Proskauer test and produced acid from methyl α--glucoside, mannitol, sorbitol and sucrose, with weak or no production of acid from methyl α--mannoside, -arabinose, gluconate and -xylose. Several of the strains were selected for identification on the basis of sequencing of almost the whole 16S rRNA gene and partial and genes and of (GTG)-PCR fingerprints. Partial and gene sequencing was also performed for those type strains of species without available sequences in the database. The pristine brook isolates formed a novel species, for which the name sp. nov. (type strain S299  = HAMBI 3055  = LMG 25899  = CCM 7986) is proposed. On the basis of 16S rRNA gene sequence similarity, sp. nov. is related to the genogoup. It is distinguished from described species on the basis of 16S rRNA, and gene sequences and whole-cell protein and (GTG)-PCR fingerprints. It is most closely related to , but DNA–DNA hybridization confirms it to represent a novel species.

Funding
This study was supported by the:
  • Ministry of Education, Youth and Sports of the Czech Republic (Award MSM0021622416)
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2012-09-01
2024-12-03
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