1887

Abstract

The taxonomic position of a Gram-negative, non-motile, oxidase negative and catalase positive strain, A648, isolated from a hexachlorocyclohexane (HCH) dump site located in Lucknow, India, was ascertained by using a polyphasic approach. A comparative analysis of a partial sequence of the gene and the 16S rRNA gene sequence revealed that strain A648 belonged to the genus . DNA–DNA relatedness values between strain A648 and other closely related members (16S rRNA gene sequence similarity greater than 97 %), namely DSM 6976, ATCC 31012, LMG 1041, LMG 21765 LMG 998 and JCM 15062, were found to be less than 8 %. The major cellular fatty acids of strain A648 were 18 : 1ω9 (19.6 %), summed feature 3 (15.9 %), 16 : 0 (10.6 %) and 12 : 0 (6.4 %). The DNA G+C content was 40.4 mol%. The polar lipid profile of strain A648 indicated the presence of diphosphatidylglycerol, phosphatidylethanolamine, followed by phosphatidylglycerol and phosphatidylcholine. The predominant polyamine of strain A648 was 1,3-diaminopropane and moderate amounts of putrescine, spermidine and spermine were also detected. The respiratory quinone consisted of ubiquinone with nine isoprene units (Q-9). On the basis of DNA–DNA hybridization, phenotypic characteristics and chemotaxonomic and phylogenetic comparisons with other members of the genus , strain A648 is found to be a novel species of the genus , for which the name sp. nov. is proposed. The type strain is A648 ( = DSM 25388 = CCM 7832).

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2012-12-01
2019-12-08
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