1887

Abstract

Two strains (JA266 and JA333) of Gram-negative, rod-shaped, phototrophic, purple non-sulfur bacteria were isolated from a freshwater fish pond and an industrial effluent. Both strains were capable of phototrophic and chemotrophic growth. Bacteriochlorophyll and carotenoids of the spirilloxanthin series were present as photosynthetic pigments. The major fatty acid for both strains was Cω7 (>65 %), with minor amounts of 11-methyl Cω7, C, Cω7 and C also present. Both strains have the lamellar type of intracellular photosynthetic membranes. Ubiquinone-10 (Q) and rhodoquinone-10 (RQ) were present as primary quinone components. Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine were the major polar lipids, while minor amounts of amino lipids (AL1, AL2) and an unidentified lipid (L1) were common to both strains. The DNA G+C contents of strains JA266 and JA333 were 71.3 and 69.9 mol%, respectively. Phylogenetic analysis on the basis of 16S rRNA gene sequences showed that both strains clustered with members of the genus in the class . Strains JA266 and JA333 had gene sequence similarity of 98.7 and 98.9 % with TUT3530, 96.4 and 96.5 % with AS130, respectively, and less than 96 % with other members of the genus . 16S rRNA gene sequence similarity between the two strains was 99.3 % and they exhibited high (84.7 %) relatedness based on DNA–DNA hybridization. Furthermore, both strains had less than 65 % DNA–DNA relatedness with the type strain TUT3530. On the basis of phenotypic and genotypic data, it is proposed that strain JA266 be classified as a novel species of the genus , with the species name sp. nov. The type strain of the proposed novel species is JA266 ( = JCM 14934 = KCTC 5627), while strain JA333 ( = NBRC 107574 = KCTC 5962) is an additional strain.

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2012-12-01
2019-08-24
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