1887

Abstract

Two strains (JA266 and JA333) of Gram-negative, rod-shaped, phototrophic, purple non-sulfur bacteria were isolated from a freshwater fish pond and an industrial effluent. Both strains were capable of phototrophic and chemotrophic growth. Bacteriochlorophyll and carotenoids of the spirilloxanthin series were present as photosynthetic pigments. The major fatty acid for both strains was Cω7 (>65 %), with minor amounts of 11-methyl Cω7, C, Cω7 and C also present. Both strains have the lamellar type of intracellular photosynthetic membranes. Ubiquinone-10 (Q) and rhodoquinone-10 (RQ) were present as primary quinone components. Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine were the major polar lipids, while minor amounts of amino lipids (AL1, AL2) and an unidentified lipid (L1) were common to both strains. The DNA G+C contents of strains JA266 and JA333 were 71.3 and 69.9 mol%, respectively. Phylogenetic analysis on the basis of 16S rRNA gene sequences showed that both strains clustered with members of the genus in the class . Strains JA266 and JA333 had gene sequence similarity of 98.7 and 98.9 % with TUT3530, 96.4 and 96.5 % with AS130, respectively, and less than 96 % with other members of the genus . 16S rRNA gene sequence similarity between the two strains was 99.3 % and they exhibited high (84.7 %) relatedness based on DNA–DNA hybridization. Furthermore, both strains had less than 65 % DNA–DNA relatedness with the type strain TUT3530. On the basis of phenotypic and genotypic data, it is proposed that strain JA266 be classified as a novel species of the genus , with the species name sp. nov. The type strain of the proposed novel species is JA266 ( = JCM 14934 = KCTC 5627), while strain JA333 ( = NBRC 107574 = KCTC 5962) is an additional strain.

Funding
This study was supported by the:
  • Department of Biotechnology, Government of India
  • CSIR, Government of India
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.037663-0
2012-12-01
2024-10-06
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/12/2828.html?itemId=/content/journal/ijsem/10.1099/ijs.0.037663-0&mimeType=html&fmt=ahah

References

  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. 1990; Basic local alignment search tool. J Mol Biol 215:403–410[PubMed] [CrossRef]
    [Google Scholar]
  2. Anil Kumar P., Srinivas T. N. R., Sasikala Ch., Ramana Ch. V. 2008; Allochromatium renukae sp. nov.. Int J Syst Evol Microbiol 58:404–407 [View Article][PubMed]
    [Google Scholar]
  3. Biebl H., Pfennig N. 1981; Isolation of members of the family Rhodospirillaceae . In The Prokaryotes: A Handbook on Habitats, Isolation, and Identification of Bacteria vol. 1 pp. 267–273 Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. G. New York: Springer;
    [Google Scholar]
  4. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [View Article][PubMed]
    [Google Scholar]
  5. Hiraishi A., Hoshino Y. 1984; Distribution of rhodoquinone in Rhodospirillaceae and its taxonomic implications. J Gen Appl Microbiol 30:435–448 [View Article]
    [Google Scholar]
  6. Hiraishi A., Ueda Y. 1994; Rhodoplanes gen. nov., a new genus of phototrophic bacteria including Rhodopseudomonas rosea as Rhodoplanes roseus comb. nov. and Rhodoplanes elegans sp. nov.. Int J Syst Bacteriol 44:665–673 [View Article]
    [Google Scholar]
  7. Imhoff J. F. 1984; Quinones of phototrophic purple bacteria. FEMS Microbiol Lett 25:85–89 [View Article]
    [Google Scholar]
  8. Imhoff J. F., Süling J., Petri R. 1998; Phylogenetic relationships among the Chromatiaceae, their taxonomic reclassification and description of the new genera Allochromatium, Halochromatium, Isochromatium, Marichromatium, Thiococcus, Thiohalocapsa and Thermochromatium . Int J Syst Bacteriol 48:1129–1143 [View Article][PubMed]
    [Google Scholar]
  9. Kates M. 1972 Techniques of Lipidology New York: Elsevier;
    [Google Scholar]
  10. Kates M. 1986 Techniques of Lipidology: Isolation, Analysis, and Identification of Lipids Amsterdam: Elsevier;
    [Google Scholar]
  11. Lakshmi K. V. N. S., Sasikala Ch., Ramana Ch. V. 2009; Rhodoplanes pokkaliisoli sp. nov., a phototrophic alphaproteobacterium isolated from a waterlogged brackish paddy soil. Int J Syst Evol Microbiol 59:2153–2157 [View Article][PubMed]
    [Google Scholar]
  12. Okamura K., Hisada T., Hiraishi A. 2007; Characterization of thermotolerant purple nonsulfur bacteria isolated from hot-spring Chloroflexus mats and the reclassification of “Rhodopseudomonas cryptolactis” Stadtwald-Demchick et al. 1990 as Rhodoplanes cryptolactis nom. rev., comb. nov.. J Gen Appl Microbiol 53:357–361 [View Article][PubMed]
    [Google Scholar]
  13. Okamura K., Kanbe T., Hiraishi A. 2009; Rhodoplanes serenus sp. nov., a purple non-sulfur bacterium isolated from pond water. Int J Syst Evol Microbiol 59:531–535 [View Article][PubMed]
    [Google Scholar]
  14. Oren A., Duker S., Ritter S. 1996; The polar lipid composition of Walsby’s square bacterium. FEMS Microbiol Lett 138:135–140 [View Article]
    [Google Scholar]
  15. Sasser M. 1990 Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.;
    [Google Scholar]
  16. Srinivas T. N. R., Kumar P. A., Sasikala Ch., Ramana Ch. V. 2007; Rhodovulum imhoffii sp. nov.. Int J Syst Evol Microbiol 57:228–232 [View Article][PubMed]
    [Google Scholar]
  17. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: Molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [View Article][PubMed]
    [Google Scholar]
  18. Tindall B. J. 1990a; Lipid composition of Halobacterium lacusprofundi . FEMS Microbiol Lett 66:199–202 [View Article]
    [Google Scholar]
  19. Tindall B. J. 1990b; A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13:128–130 [View Article]
    [Google Scholar]
  20. Tourova T. P., Antonov A. S. 1988; Identification of microorganisms by rapid DNA-DNA hybridization. Methods in Microbiol 19:333–355 [View Article]
    [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.037663-0
Loading
/content/journal/ijsem/10.1099/ijs.0.037663-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error