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Abstract

A pinkish-pigmented, Gram-stain-negative, rod-shaped, non-spore-forming bacterium, strain E92, was isolated from the skin of the medical leech , on R2A agar. 16S rRNA gene sequence analysis of strain E92 showed a relatively low 16S rRNA gene sequence similarity (93.0–93.5 %) to representatives of the genus and 91.5–92.0 % to members of the genus . The polar lipid profile was composed of the major compounds phosphatidylethanolamine, an unidentified aminophospholipid, an unidentified aminolipid and an unidentified polar lipid; glycolipids were not detected. The major quinone was menquinone MK-7, and the major compound in the polyamine pattern was spermidine. The predominant fatty acids were Cω5 and Cω7 and/or iso-C 2-OH (detected as summed feature 3). The isolate did not contain C or the hydroxyl fatty acid iso-C 3-OH found in all representatives of the genera and , but did produce Cω7 and 11-methyl Cω7 which are not found in these two genera The DNA G+C content of strain E92 was 64.4 mol%. The unique 16S rRNA gene sequence, and specific chemotaxonomic and physiological data revealed that strain E92 represents a new genus in the family for which we propose the name gen. nov., sp. nov., with the type strain of the type species as E92 ( = LMG 26720 = CCM 7988).

Funding
This study was supported by the:
  • Spanish Ministry of Science and Innovation (Award CE-CSD2007-0005, CLG2009_12651-C02-02 and CLG2009_12651-C02-01)
  • FEDER support from the European Union
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2012-09-01
2021-10-19
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