1887

Abstract

A group of five Gram-negative, aerobic, halotolerant, non-pigmented bacteria isolated from shallow sediment samples and invertebrate specimens collected from the Gulf of Alaska and the Sea of Japan was subjected to taxonomic study. On the basis of 16S rRNA gene sequence analysis, the novel isolates were affiliated to the genus , sharing the highest sequence similarity of 99.3–99.9 % with DSM 4741. DNA–DNA hybridization experiments between and among the novel strains and DSM 4741 and JCM 15644 revealed that the five strains represent three separate genospecies, which could be differentiated in their morphological, physiological and biochemical characteristics. NBRC 15607 was included in this study as it has recently been reported to exhibit high 16S rRNA gene sequence similarity to DSM 4741, and it showed a high DNA relatedness value of 96 % with DSM 4741, indicating that they belong to the same species. On the basis of phylogenetic analysis, DNA–DNA hybridization and phenotypic characterization, three novel species are proposed, named sp. nov. (type strain KMM 1561  = NRIC 0815  = CCUG 49560), sp. nov. (type strain KMM 3880  = NRIC 0814  = CCUG 49563) and sp. nov. (type strain KMM 3879  = NRIC 0813  = CCUG 49562). It is also proposed that is a later heterotypic synonym of , and emended descriptions of the genus and the species are provided.

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2013-01-01
2019-12-11
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