1887

Abstract

A Gram-negative, rod-shaped, weakly motile, non-spore-forming bacterium (D9) was isolated from the gut of (Diplopoda) on 1/3-strength nutrient agar plates. On the basis of 16S rRNA gene sequence similarity, strain D9 was shown to be phylogenetically closely related to the type strain of , the sole species of the genus , family The similarity of the 16S rRNA gene sequences of train D9 and DSM 5075 was 98.4 %. Other strains that showed high pairwise similarities with the isolate belonged to the genus : ATCC 33641 (96.8 % 16S rRNA gene sequence similarity), CCUG 53443 (96.8 %), NCTC 5923 (96.8 %), ATCC 29833 (96.8 %), CCUG 52882 (96.7 %) and ATCC 29473 (96.5 % ). The similarities of sequences of the housekeeping genes , and between strain D9 and DSM 5075 and other members of the were less than 94 %. Phylogenetic trees based on all four gene sequences unequivocally grouped the isolate with the type strain of and separately from the genus . Cells contained the quinones Q-8, Q-7 and MK-8. The major polar lipids were phosphatidylglycerol and phosphatidylethanolamine. The G+C content of the DNA (48.3 mol%) and the whole-cell fatty acid composition of strain D9 (C, Cω7, C, cyclo-C and Cω7 as major components) were typical for members of the . DNA–DNA hybridization of strain D9 with DSM 5075 resulted in a relatedness of 30.4 %, indicating that the isolate did not belong to . Physiological tests allowed the phenotypic differentiation of strain D9 from DSM 5075 as well as from members of the genus From these results, it is concluded that strain D9 represents a novel species, for which the name sp. nov. is proposed (type strain D9  = DSM 21983  = CCM 7845). The description of the genus is emended.

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2013-01-01
2021-10-21
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