1887

Abstract

A novel, Gram-staining-negative, yellow-coloured, rod-shaped, obligately aerobic, non-motile bacterium, designated strain AK7, was isolated from seawater collected on the coast at Visakhapatnam, Andhra Pradesh, India. The predominant fatty acids of the novel strain were iso-C, iso-C 3-OH, Cω5, iso-C 3-OH and summed features 3 (Cω7 and/or iso-C 2-OH) and 4 (iso-C I and/or anteiso-C B). The major respiratory quinone was MK-7 and the polar lipid profile comprised phosphatidylethanolamine, two unidentified aminolipids and four other unidentified lipids. In phylogenetic analysis based on 16S rRNA gene sequences, strain AK7 appeared most closely related to KMM 6220 (95.9 % sequence similarity), a member of the family in the phylum . The genomic DNA G+C content of strain AK7 was 55.1 mol%. Based on the morphological, biochemical, physiological, chemotaxonomic and phylogenetic evidence, strain AK7 represents a novel species of the genus for which the name sp. nov. is proposed. The type strain is AK7 ( = MTCC 11053  = JCM 17390).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.036608-0
2012-09-01
2019-10-22
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/9/2213.html?itemId=/content/journal/ijsem/10.1099/ijs.0.036608-0&mimeType=html&fmt=ahah

References

  1. Altschul S. F. , Gish W. , Miller W. , Myers E. W. , Lipman D. J. . ( 1990; ). Basic local alignment search tool. . J Mol Biol 215:, 403–410.[PubMed] [CrossRef]
    [Google Scholar]
  2. Buczolits S. , Denner E. B. M. , Vybiral D. , Wieser M. , Kämpfer P. , Busse H.-J. . ( 2002; ). Classification of three airborne bacteria and proposal of Hymenobacter aerophilus sp. nov.. Int J Syst Evol Microbiol 52:, 445–456.[PubMed]
    [Google Scholar]
  3. Chun J. , Lee J.-H. , Jung Y. , Kim M. , Kim S. , Kim B. K. , Lim Y. W. . ( 2007; ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. . Int J Syst Evol Microbiol 57:, 2259–2261. [CrossRef] [PubMed]
    [Google Scholar]
  4. Collins M. D. , Jones D. . ( 1980; ). Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4-diaminobutyric acid. . J Appl Bacteriol 48:, 459–470.[CrossRef]
    [Google Scholar]
  5. Collins M. D. , Pirouz T. , Goodfellow M. , Minnikin D. E. . ( 1977; ). Distribution of menaquinones in actinomycetes and corynebacteria. . J Gen Microbiol 100:, 221–230.[PubMed] [CrossRef]
    [Google Scholar]
  6. Cowan S. T , Steel K. J. . ( 1965; ). Manual for the Identification of Medical Bactria. London: Cambridge University Press. .
    [Google Scholar]
  7. Groth I. , Schumann P. , Weiss N. , Martin K. , Rainey F. A. . ( 1996; ). Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. . Int J Syst Bacteriol 46:, 234–239. [CrossRef] [PubMed]
    [Google Scholar]
  8. Hall T. A. . ( 1999; ). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. . Nucleic Acids Symp Ser 41:, 95–98.
    [Google Scholar]
  9. Marmur J. . ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. . J Mol Biol 3:, 208–218. [CrossRef]
    [Google Scholar]
  10. Nedashkovskaya O. I. , Kim S. B. , Shin D. S. , Beleneva I. A. , Mikhailov V. V. . ( 2007; ). Fulvivirga kasyanovii gen. nov., sp. nov., a novel member of the phylum Bacteroidetes isolated from seawater in a mussel farm. . Int J Syst Evol Microbiol 57:, 1046–1049. [CrossRef] [PubMed]
    [Google Scholar]
  11. O’Sullivan L. A. , Rinna J. , Humphreys G. , Weightman A. J. , Fry J. C. . ( 2006; ). Culturable phylogenetic diversity of the phylum ‘Bacteroidetes’ from river epilithon and coastal water and description of novel members of the family Flavobacteriaceae: Epilithonimonas tenax gen. nov., sp. nov. and Persicivirga xylanidelens gen. nov., sp. nov.. Int J Syst Evol Microbiol 56:, 169–180. [CrossRef] [PubMed]
    [Google Scholar]
  12. Sly L. I. , Blackall L. L. , Kraat P. C. , Tian-Shen T. , Sangkhobol V. . ( 1986; ). The use of second derivative plots for the determination of mol% guanine plus cytosine of DNA by the thermal denaturation method. . J Microbiol Methods 5:, 139–156. [CrossRef]
    [Google Scholar]
  13. Tamura K. , Peterson D. , Peterson N. , Stecher G. , Nei M. , Kumar S. . ( 2011; ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef] [PubMed]
    [Google Scholar]
  14. Thompson J. D. , Higgins D. G. , Gibson T. J. . ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. . Nucleic Acids Res 22:, 4673–4680. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.036608-0
Loading
/content/journal/ijsem/10.1099/ijs.0.036608-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error