1887

Abstract

A novel Gram-staining-positive, rod-shaped, motile, strictly aerobic, endospore-forming bacterium, designated WCC 4585, was isolated from a pharmaceutical production line. The organism grew optimally at 30 °C, at pH 8 and in the presence of 0.5 % (w/v) NaCl. Oval endospores were formed subterminally and terminally in swollen sporangia. The cell-wall diamino acid was -diaminopimelic acid (type A1γ) and the genomic DNA G+C content was 38.7 mol%. The major menaquinone was MK-7. The cellular fatty acid profile contained major amounts of iso-C, anteiso-C and anteiso-C, and the cellular phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and aminophospholipid. The isolate was most closely related to H2, SMC 4352-1, NCIMB 9366, ATCC 4513 and DSM 23495 with which it shared less than 98.0 % 16S rRNA gene sequence similarity. DNA–DNA relatedness values between strain WCC 4585 and five type strains of related species were ≤27 % and sequence similarity values based on sequences were ≤88.7 %. On the basis of the characteristics presented, strain WCC 4585 is proposed to represent a novel species, sp. nov. The type strain is WCC 4585( = DSM 23668 = CCUG 59876 = LMG 25856).

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2013-03-01
2019-10-23
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