1887

Abstract

A novel actinobacterial strain, MBRL 353, was isolated from a sample collected from a limestone quarry at Hundung, Manipur, India. Comparison of 16S rRNA gene sequences of strain MBRL 353 and other members of the genus showed sequence similarities ranging from 95.5 to 98.2 %, with strain MBRL 353 showing closest sequence similarity to IMMIB RIV-085 (98.2 %) and DSM 20307 (97.2 %). DNA–DNA hybridization results, however, revealed that DNA–DNA relatedness values between strain MBRL 353 and DSM 44892 (43.4 %) and DSM 20307 (33.4 %) were well below the 70 % limit for species identification. Strain MBRL 353 contained -diaminopimelic acid as the diagnostic diamino acid and galactose and arabinose in the cell wall. Mycolic acids were present. The major fatty acids were C (45.7 %), Cω9 (18.2 %) and 10-methyl C (11.3 %). The only menaquinone detected was MK-8(H), while the major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside and one unknown phospholipid. The G+C content of the genomic DNA was 69.2 mol%. The phenotypic and genotypic data showed that strain MBRL 353 merits recognition as a representative of a novel species of the genus for which the name sp. nov. is proposed; the type strain is MBRL 353 ( = KCTC 19851 = JCM 17578).

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2013-01-01
2020-01-19
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