1887

Abstract

A Gram-staining-negative, aerobic, motile, oxidase- and catalase-positive, rod-shaped strain, designated ZS2-30, was isolated from Antarctic intertidal sandy sediment. The strain grew at 4–35 °C (optimum, 25 °C) and in 0–25 % (w/v) NaCl (optimum, 3.0–4.0 %). It could reduce nitrate to nitrite and hydrolyse Tween 80. The predominant cellular fatty acids of strain ZS2-30 were summed feature 3 (Cω7 and/or Cω6), C, Cω9, Cω9, C 3-OH and C. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and an unidentified aminophospholipid. The genomic DNA G+C content of strain ZS2-30 was 55.8 mol%. Analyses of 16S rRNA gene sequences revealed that strain ZS2-30 was affiliated with the genus . It showed highest 16S rRNA gene sequence similarities to the type strains of three species of the genus , namely (98.3 %), (98.1 %) and (97.1 %), but the DNA–DNA relatedness values between strain ZS2-30 and the above three species were all lower than 45 %. Moreover, strain ZS2-30 could be distinguished from closely related species of the genus by various phenotypic properties. Based on this taxonomic study using a polyphasic approach, strain ZS2-30 is considered to represent a novel species in the genus , for which the name sp. nov. is proposed. The type strain of is ZS2-30 ( = CGMCC 1.10835 = KCTC 23684).

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2012-08-01
2019-10-17
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