1887

Abstract

A Gram-staining-negative, aerobic, motile, oxidase- and catalase-positive, rod-shaped strain, designated ZS2-30, was isolated from Antarctic intertidal sandy sediment. The strain grew at 4–35 °C (optimum, 25 °C) and in 0–25 % (w/v) NaCl (optimum, 3.0–4.0 %). It could reduce nitrate to nitrite and hydrolyse Tween 80. The predominant cellular fatty acids of strain ZS2-30 were summed feature 3 (Cω7 and/or Cω6), C, Cω9, Cω9, C 3-OH and C. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and an unidentified aminophospholipid. The genomic DNA G+C content of strain ZS2-30 was 55.8 mol%. Analyses of 16S rRNA gene sequences revealed that strain ZS2-30 was affiliated with the genus . It showed highest 16S rRNA gene sequence similarities to the type strains of three species of the genus , namely (98.3 %), (98.1 %) and (97.1 %), but the DNA–DNA relatedness values between strain ZS2-30 and the above three species were all lower than 45 %. Moreover, strain ZS2-30 could be distinguished from closely related species of the genus by various phenotypic properties. Based on this taxonomic study using a polyphasic approach, strain ZS2-30 is considered to represent a novel species in the genus , for which the name sp. nov. is proposed. The type strain of is ZS2-30 ( = CGMCC 1.10835 = KCTC 23684).

Funding
This study was supported by the:
  • , National Natural Science Foundation of China , (Award 31070061, 31025001 and 40876072)
  • , Hi-Tech Research and Development program of China , (Award 2011AA090703)
  • , Special Fund of China for Marine-scientific Research in the Public Interest , (Award 201005032-6)
  • , Natural Science Foundation of Shandong Province, China , (Award ZR2009DZ002 and JQ200910)
  • , Foundation for Young Excellent Scientists in Shandong Province, China , (Award BS2010SW015 and 2007BS07007)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.035774-0
2012-08-01
2021-03-07
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/8/1838.html?itemId=/content/journal/ijsem/10.1099/ijs.0.035774-0&mimeType=html&fmt=ahah

References

  1. Aguilera M., Jiménez-Pranteda M. L., Kharroub K., González-Paredes A., Durban J. J., Russell N. J., Ramos-Cormenzana A., Monteoliva-Sánchez M. 2009; Marinobacter lacisalsi sp. nov., a moderately halophilic bacterium isolated from the saline-wetland wildfowl reserve Fuente de Piedra in southern Spain. Int J Syst Evol Microbiol 59:1691–1695 [CrossRef][PubMed]
    [Google Scholar]
  2. Antunes A., França L., Rainey F. A., Huber R., Nobre M. F., Edwards K. J., da Costa M. S. 2007; Marinobacter salsuginis sp. nov., isolated from the brine-seawater interface of the Shaban Deep, Red Sea. Int J Syst Evol Microbiol 57:1035–1040 [CrossRef][PubMed]
    [Google Scholar]
  3. Buck J. D. 1982; Nonstaining (KOH) method for determination of gram reactions of marine bacteria. Appl Environ Microbiol 44:992–993[PubMed]
    [Google Scholar]
  4. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [CrossRef][PubMed]
    [Google Scholar]
  5. Collins M. D., Jones D. 1980; Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4-diaminobutyric acid. J Appl Bacteriol 48:459–470 [CrossRef]
    [Google Scholar]
  6. Cowan S. T., Steel K. J. 1965 Manual for the Identification of Medical Bacteria London: Cambridge University Press;
    [Google Scholar]
  7. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [CrossRef][PubMed]
    [Google Scholar]
  8. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  9. Gauthier M. J., Lafay B., Christen R., Fernandez L., Acquaviva M., Bonin P., Bertrand J. C. 1992; Marinobacter hydrocarbonoclasticus gen. nov., sp. nov., a new, extremely halotolerant, hydrocarbon-degrading marine bacterium. Int J Syst Bacteriol 42:568–576 [CrossRef][PubMed]
    [Google Scholar]
  10. Gorshkova N. M., Ivanova E. P., Sergeev A. F., Zhukova N. V., Alexeeva Y., Wright J. P., Nicolau D. V., Mikhailov V. V., Christen R. 2003; Marinobacter excellens sp. nov., isolated from sediments of the Sea of Japan. Int J Syst Evol Microbiol 53:2073–2078 [CrossRef][PubMed]
    [Google Scholar]
  11. Green D. H., Bowman J. P., Smith E. A., Gutierrez T., Bolch C. J. S. 2006; Marinobacter algicola sp. nov., isolated from laboratory cultures of paralytic shellfish toxin-producing dinoflagellates. Int J Syst Evol Microbiol 56:523–527 [CrossRef][PubMed]
    [Google Scholar]
  12. Gu J., Cai H., Yu S. L., Qu R., Yin B., Guo Y. F., Zhao J. Y., Wu X. L. 2007; Marinobacter gudaonensis sp. nov., isolated from an oil-polluted saline soil in a Chinese oilfield. Int J Syst Evol Microbiol 57:250–254 [CrossRef][PubMed]
    [Google Scholar]
  13. Guo B., Gu J., Ye Y. G., Tang Y. Q., Kida K., Wu X. L. 2007; Marinobacter segnicrescens sp. nov., a moderate halophile isolated from benthic sediment of the South China Sea. Int J Syst Evol Microbiol 57:1970–1974 [CrossRef][PubMed]
    [Google Scholar]
  14. Handley K. M., Héry M., Lloyd J. R. 2009; Marinobacter santoriniensis sp. nov., an arsenate-respiring and arsenite-oxidizing bacterium isolated from hydrothermal sediment. Int J Syst Evol Microbiol 59:886–892 [CrossRef][PubMed]
    [Google Scholar]
  15. Huo Y. Y., Wang C. S., Yang J. Y., Wu M., Xu X. W. 2008; Marinobacter mobilis sp. nov. and Marinobacter zhejiangensis sp. nov., halophilic bacteria isolated from the East China Sea. Int J Syst Evol Microbiol 58:2885–2889 [CrossRef][PubMed]
    [Google Scholar]
  16. Huß V. A. R., Festl H., Schleifer K. H. 1983; Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192 [CrossRef]
    [Google Scholar]
  17. Huu N. B., Denner E. B. M., Ha D. T. C., Wanner G., Stan-Lotter H. 1999; Marinobacter aquaeolei sp. nov., a halophilic bacterium isolated from a Vietnamese oil-producing well. Int J Syst Bacteriol 49:367–375 [CrossRef][PubMed]
    [Google Scholar]
  18. Kaeppel E. C., Gärdes A., Seebah S., Grossart H.-P., Ullrich M. S. 2012; Marinobacter adhaerens sp. nov., isolated from marine aggregates formed with the diatom Thalassiosira weissflogii . Int J Syst Evol Microbiol 62:124–128 [CrossRef][PubMed]
    [Google Scholar]
  19. Kharroub K., Aguilera M., Jiménez-Pranteda M. L., González-Paredes A., Ramos-Cormenzana A., Monteoliva-Sánchez M. 2011; Marinobacter oulmenensis sp. nov., a moderately halophilic bacterium isolated from brine of a salt concentrator. Int J Syst Evol Microbiol 61:2210–2214 [CrossRef][PubMed]
    [Google Scholar]
  20. Kim B. Y., Weon H. Y., Yoo S. H., Kim J. S., Kwon S. W., Stackebrandt E., Go S. J. 2006; Marinobacter koreensis sp. nov., isolated from sea sand in Korea. Int J Syst Evol Microbiol 56:2653–2656 [CrossRef][PubMed]
    [Google Scholar]
  21. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef][PubMed]
    [Google Scholar]
  22. Komagata K., Suzuki K. 1987; Lipid and cell wall analysis in bacterial systematics. Methods Microbiol 19:161–207 [CrossRef]
    [Google Scholar]
  23. Lane D. J. 1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp. 115–175 Edited by Stackebrandt E., Goodfellow M. Chichester: Wiley;
    [Google Scholar]
  24. Liebgott P. P., Casalot L., Paillard S., Lorquin J., Labat M. 2006; Marinobacter vinifirmus sp. nov., a moderately halophilic bacterium isolated from a wine-barrel-decalcification wastewater. Int J Syst Evol Microbiol 56:2511–2516 [CrossRef][PubMed]
    [Google Scholar]
  25. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 3:208–218 [CrossRef]
    [Google Scholar]
  26. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [CrossRef][PubMed]
    [Google Scholar]
  27. Márquez M. C., Ventosa A. 2005; Marinobacter hydrocarbonoclasticus Gauthier et al. 1992 and Marinobacter aquaeolei Nguyen et al. 1999 are heterotypic synonyms. Int J Syst Evol Microbiol 55:1349–1351 [CrossRef][PubMed]
    [Google Scholar]
  28. Martín S., Márquez M. C., Sánchez-Porro C., Mellado E., Arahal D. R., Ventosa A. 2003; Marinobacter lipolyticus sp. nov., a novel moderate halophile with lipolytic activity. Int J Syst Evol Microbiol 53:1383–1387 [CrossRef][PubMed]
    [Google Scholar]
  29. Montes M. J., Bozal N., Mercadé E. 2008; Marinobacter guineae sp. nov., a novel moderately halophilic bacterium from an Antarctic environment. Int J Syst Evol Microbiol 58:1346–1349 [CrossRef][PubMed]
    [Google Scholar]
  30. Murray R. G. E., Doetsch R. N., Robinow C. F. 1994; Determinative and cytological light microscopy. In Methods for General and Molecular Bacteriology pp. 21–41 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  31. Qu L. Y., Zhu F. L., Zhang J. X., Gao C. L., Sun X. Q. 2011; Marinobacter daqiaonensis sp. nov., a moderate halophile isolated from a Yellow Sea salt pond. Int J Syst Evol Microbiol 61:3003–3008 [CrossRef][PubMed]
    [Google Scholar]
  32. Roh S. W., Quan Z. X., Nam Y. D., Chang H. W., Kim K. H., Rhee S. K., Oh H. M., Jeon C. O., Yoon J. H., Bae J. W. 2008; Marinobacter goseongensis sp. nov., from seawater. Int J Syst Evol Microbiol 58:2866–2870 [CrossRef][PubMed]
    [Google Scholar]
  33. Romanenko L. A., Schumann P., Rohde M., Zhukova N. V., Mikhailov V. V., Stackebrandt E. 2005; Marinobacter bryozoorum sp. nov. and Marinobacter sediminum sp. nov., novel bacteria from the marine environment. Int J Syst Evol Microbiol 55:143–148 [CrossRef][PubMed]
    [Google Scholar]
  34. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  35. Shieh W. Y., Jean W. D., Lin Y. T., Tseng M. 2003; Marinobacter lutaoensis sp. nov., a thermotolerant marine bacterium isolated from a coastal hot spring in Lutao, Taiwan. Can J Microbiol 49:244–252 [CrossRef][PubMed]
    [Google Scholar]
  36. Shieh W. Y., Lin Y. T., Jean W. D. 2004; Pseudovibrio denitrificans gen. nov., sp. nov., a marine, facultatively anaerobic, fermentative bacterium capable of denitrification. Int J Syst Evol Microbiol 54:2307–2312 [CrossRef][PubMed]
    [Google Scholar]
  37. Shivaji S., Gupta P., Chaturvedi P., Suresh K., Delille D. 2005; Marinobacter maritimus sp. nov., a psychrotolerant strain isolated from sea water off the subantarctic Kerguelen islands. Int J Syst Evol Microbiol 55:1453–1456 [CrossRef][PubMed]
    [Google Scholar]
  38. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp. 607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  39. Spröer C., Lang E., Hobeck P., Burghardt J., Stackebrandt E., Tindall B. J. 1998; Transfer of Pseudomonas nautica to Marinobacter hydrocarbonoclasticus . Int J Syst Bacteriol 48:1445–1448 [CrossRef]
    [Google Scholar]
  40. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  41. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [CrossRef][PubMed]
    [Google Scholar]
  42. Wang C. Y., Ng C. C., Tzeng W. S., Shyu Y. T. 2009; Marinobacter szutsaonensis sp. nov., isolated from a solar saltern. Int J Syst Evol Microbiol 59:2605–2609 [CrossRef][PubMed]
    [Google Scholar]
  43. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  44. Xu X. W., Wu Y. H., Wang C. S., Yang J. Y., Oren A., Wu M. 2008; Marinobacter pelagius sp. nov., a moderately halophilic bacterium. Int J Syst Evol Microbiol 58:637–640 [CrossRef][PubMed]
    [Google Scholar]
  45. Yoon J. H., Shin D. Y., Kim I. G., Kang K. H., Park Y. H. 2003; Marinobacter litoralis sp. nov., a moderately halophilic bacterium isolated from sea water from the East Sea in Korea. Int J Syst Evol Microbiol 53:563–568 [CrossRef][PubMed]
    [Google Scholar]
  46. Yoon J. H., Yeo S. H., Kim I. G., Oh T. K. 2004; Marinobacter flavimaris sp. nov. and Marinobacter daepoensis sp. nov., slightly halophilic organisms isolated from sea water of the Yellow Sea in Korea. Int J Syst Evol Microbiol 54:1799–1803 [CrossRef][PubMed]
    [Google Scholar]
  47. Yoon J. H., Lee M. H., Kang S. J., Oh T. K. 2007; Marinobacter salicampi sp. nov., isolated from a marine solar saltern in Korea. Int J Syst Evol Microbiol 57:2102–2105 [CrossRef][PubMed]
    [Google Scholar]
  48. Yu Y., Li H. R., Zeng Y. X., Chen B. 2010; Phylogenetic diversity of culturable bacteria from Antarctic sandy intertidal sediments. Polar Biol 33:869–875 [CrossRef]
    [Google Scholar]
  49. Zhang D. C., Li H. R., Xin Y. H., Chi Z. M., Zhou P. J., Yu Y. 2008; Marinobacter psychrophilus sp. nov., a psychrophilic bacterium isolated from the Arctic. Int J Syst Evol Microbiol 58:1463–1466 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.035774-0
Loading
/content/journal/ijsem/10.1099/ijs.0.035774-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error