1887

Abstract

A bacterial strain designated SW15 was isolated from a sample of the reef-building coral , collected in southern Taiwan. The novel strain was characterized using a polyphasic taxonomic approach. Cells of strain SW15 were Gram-negative, aerobic, light yellow, rod-shaped and motile by means of a single polar flagellum. In phylogenetic analyses based on 16S rRNA gene sequences, strain SW15 appeared to belong to the genus in the class and to be most closely related to PO-M2 (97.6 % sequence similarity). Strain SW15 exhibited optimal growth between 20 and 30 °C, with NaCl between 3 % and 4 % (w/v) and at a pH value between 7 and 8. Predominant cellular fatty acids were iso-C (31.1 %), iso-C (15.4 %), iso-Cω9 (10.0 %) and C (8.8 %). The major respiratory quinone was ubiquinone Q-8. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylserine, an uncharacterized aminolipid and several uncharacterized phospholipids. The DNA G+C content was 51.1 mol%. The level of DNA–DNA relatedness between strain SW15 and PO-M2 was 42.6–56.5 %. The results of physiological and biochemical tests allowed the clear phenotypic differentiation of the novel strain from established species of the genus . Based on the genotypic, phenotypic and chemotaxonomic data, strain SW15 represents a novel species in the genus , for which the name sp. nov. is proposed, with SW15 ( = LMG 25374 = BCRC 80083) as the type strain.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.035592-0
2012-07-01
2019-10-16
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/7/1536.html?itemId=/content/journal/ijsem/10.1099/ijs.0.035592-0&mimeType=html&fmt=ahah

References

  1. Altenburger P. , Kämpfer P. , Makristathis A. , Lubitz W. , Busse H.-J. . ( 1996; ). Classification of bacteria isolated from a medieval wall painting. . J Biotechnol 47:, 39–52. [CrossRef]
    [Google Scholar]
  2. Anzai Y. , Kudo Y. , Oyaizu H. . ( 1997; ). The phylogeny of the genera Chryseomonas, Flavimonas, and Pseudomonas supports synonymy of these three genera. . Int J Syst Bacteriol 47:, 249–251. [CrossRef] [PubMed]
    [Google Scholar]
  3. Bowman J. P. . ( 2000; ). Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov.. Int J Syst Evol Microbiol 50:, 1861–1868.[PubMed]
    [Google Scholar]
  4. Chen W. M. , Laevens S. , Lee T. M. , Coenye T. , De Vos P. , Mergeay M. , Vandamme P. . ( 2001; ). Ralstonia taiwanensis sp. nov., isolated from root nodules of Mimosa species and sputum of a cystic fibrosis patient. . Int J Syst Evol Microbiol 51:, 1729–1735. [CrossRef] [PubMed]
    [Google Scholar]
  5. Chun J. , Lee J.-H. , Jung Y. , Kim M. , Kim S. , Kim B. K. , Lim Y. W. . ( 2007; ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. . Int J Syst Evol Microbiol 57:, 2259–2261. [CrossRef] [PubMed]
    [Google Scholar]
  6. Collins M. D. . ( 1985; ). Analysis of isoprenoid quinones. . Methods Microbiol 18:, 329–366. [CrossRef]
    [Google Scholar]
  7. Ezaki T. , Hashimoto Y. , Yabuuchi E. . ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39:, 224–229. [CrossRef]
    [Google Scholar]
  8. Felsenstein J. . ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef] [PubMed]
    [Google Scholar]
  9. Felsenstein J. . ( 1993; ). phylip (phylogeny inference package) version 3.5.1. Distributed by the author.. Department of Genome Sciences, University of Washington, Seattle, USA.
  10. Gerhardt P. , Murray R. G. E. , Wood W. A. , Krieg N. R. . (editors) ( 1994; ). Methods for General and Molecular Bacteriology. Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  11. Hall T. A. . ( 1999; ). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. . Nucleic Acids Symp Ser 41:, 95–98.
    [Google Scholar]
  12. Hosoya S. , Adachi K. , Kasai H. . ( 2009; ). Thalassomonas actiniarum sp. nov. and Thalassomonas haliotis sp. nov., isolated from marine animals. . Int J Syst Evol Microbiol 59:, 686–690. [CrossRef] [PubMed]
    [Google Scholar]
  13. Hsu S. C. , Lockwood J. L. . ( 1975; ). Powdered chitin agar as a selective medium for enumeration of actinomycetes in water and soil. . Appl Microbiol 29:, 422–426.[PubMed]
    [Google Scholar]
  14. Hu Z.-Y. , Li Y. . ( 2007; ). Pseudidiomarina sediminum sp. nov., a marine bacterium isolated from coastal sediments of Luoyuan Bay in China. . Int J Syst Evol Microbiol 57:, 2572–2577. [CrossRef] [PubMed]
    [Google Scholar]
  15. Ivanova E. P. , Romanenko L. A. , Chun J. , Matte M. H. , Matte G. R. , Mikhailov V. V. , Svetashev V. I. , Huq A. , Maugel T. , Colwell R. R. . ( 2000; ). Idiomarina gen. nov., comprising novel indigenous deep-sea bacteria from the Pacific Ocean, including descriptions of two species, Idiomarina abyssalis sp. nov. and Idiomarina zobellii sp. nov.. Int J Syst Evol Microbiol 50:, 901–907. [CrossRef] [PubMed]
    [Google Scholar]
  16. Jean W. D. , Shieh W. Y. , Chiu H.-H. . ( 2006; ). Pseudidiomarina taiwanensis gen. nov., sp. nov., a marine bacterium isolated from shallow coastal water of An-Ping Harbour, Taiwan, and emended description of the family Idiomarinaceae . . Int J Syst Evol Microbiol 56:, 899–905. [CrossRef] [PubMed]
    [Google Scholar]
  17. Jean W. D. , Leu T. Y. , Lee C.-Y. , Chu T. J. , Lin S. Y. , Shieh W. Y. . ( 2009; ). Pseudidiomarina marina sp. nov. and Pseudidiomarina tainanensis sp. nov. and reclassification of Idiomarina homiensis and Idiomarina salinarum as Pseudidiomarina homiensis comb. nov. and Pseudidiomarina salinarum comb. nov., respectively. . Int J Syst Evol Microbiol 59:, 53–59. [CrossRef] [PubMed]
    [Google Scholar]
  18. Kimura M. . ( 1983; ). The Neutral Theory of Molecular Evolution. Cambridge:: Cambridge University Press;.[CrossRef]
    [Google Scholar]
  19. Kluge A. G. , Farris J. S. . ( 1969; ). Quantitative phyletics and the evolution of anurans. . Syst Zool 18:, 1–32. [CrossRef]
    [Google Scholar]
  20. Kwon S.-W. , Kim B.-Y. , Weon H.-Y. , Baek Y.-K. , Koo B.-S. , Go S.-J. . ( 2006; ). Idiomarina homiensis sp. nov., isolated from seashore sand in Korea. . Int J Syst Evol Microbiol 56:, 2229–2233. [CrossRef] [PubMed]
    [Google Scholar]
  21. Lyman J. , Fleming R. H. . ( 1940; ). Composition of sea water. . J Mar Res 3:, 134–146.
    [Google Scholar]
  22. MacFaddin J. F. . ( 2000; ). Biochemical Tests for the Identification of Medical Bacteria, , 3rd edn.. Baltimore, MD:: Williams & Wilkins;.
    [Google Scholar]
  23. Maidak B. L. , Cole J. R. , Lilburn T. G. , Parker C. T. Jr , Saxman P. R. , Farris R. J. , Garrity G. M. , Olsen G. J. , Schmidt T. M. , Tiedje J. M. . ( 2001; ). The RDP-II (Ribosomal Database Project). . Nucleic Acids Res 29:, 173–174. [CrossRef] [PubMed]
    [Google Scholar]
  24. Mesbah M. , Premachandran U. , Whitman W. B. . ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  25. Minnikin D. E. , O’Donnell A. G. , Goodfellow M. , Alderson G. , Athalye M. , Schaal A. , Parlett J. H. . ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  26. Nokhal T. H. , Schlegel H. G. . ( 1983; ). Taxonomic study of Paracoccus denitrificans . . Int J Syst Bacteriol 33:, 26–37. [CrossRef]
    [Google Scholar]
  27. Powers E. M. . ( 1995; ). Efficacy of the Ryu nonstaining KOH technique for rapidly determining gram reactions of food-borne and waterborne bacteria and yeasts. . Appl Environ Microbiol 61:, 3756–3758.[PubMed]
    [Google Scholar]
  28. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  29. Sasser M. . ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101.. Newark, DE:: MIDI Inc.;
  30. Taborda M. , Antunes A. , Tiago I. , Veríssimo A. , Nobre M. F. , da Costa M. S. . ( 2009; ). Description of Idiomarina insulisalsae sp. nov., isolated from the soil of a sea salt evaporation pond, proposal to transfer the species of the genus Pseudidiomarina to the genus Idiomarina and emended description of the genus Idiomarina . . Syst Appl Microbiol 32:, 371–378. [CrossRef] [PubMed]
    [Google Scholar]
  31. Tamura K. , Peterson D. , Peterson N. , Stecher G. , Nei M. , Kumar S. . ( 2011; ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef] [PubMed]
    [Google Scholar]
  32. Thompson J. D. , Gibson T. J. , Plewniak F. , Jeanmougin F. , Higgins D. G. . ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef] [PubMed]
    [Google Scholar]
  33. Tindall B. J. . ( 1990; ). Lipid composition of Halobacterium lacusprofundi . . FEMS Microbiol Lett 66:, 199–202. [CrossRef]
    [Google Scholar]
  34. Wang L. , Lai Q. , Fu Y. , Chen H. , Wang W. , Wang J. , Sun F. , Shao Z. . ( 2011; ). Idiomarina xiamenensis sp. nov., isolated from surface seawater, and proposal to transfer Pseudidiomarina aestuarii to the genus Idiomarina as Idiomarina aestuarii comb. nov.. Int J Syst Evol Microbiol 61:, 969–973. [CrossRef] [PubMed]
    [Google Scholar]
  35. Wayne L. G. , Brenner D. J. , Colwell R. R. , Grimont P. A. D. , Kandler O. , Krichevsky M. I. , Moore L. H. , Moore W. E. C. , Murray R. G. E. . & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
  36. Weisburg W. G. , Barns S. M. , Pelletier D. A. , Lane D. J. . ( 1991; ). 16S ribosomal DNA amplification for phylogenetic study. . J Bacteriol 173:, 697–703.[PubMed]
    [Google Scholar]
  37. Yoon J.-H. , Jung S.-Y. , Jung Y.-T. , Oh T.-K. . ( 2007; ). Idiomarina salinarum sp. nov., isolated from a marine solar saltern in Korea. . Int J Syst Evol Microbiol 57:, 2503–2506. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.035592-0
Loading
/content/journal/ijsem/10.1099/ijs.0.035592-0
Loading

Data & Media loading...

Supplements

Supplementary material 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error