1887

Abstract

An actinobacterial strain, SCSIO 10427, was isolated from a gorgonian coral sample collected from Weizhou Island, Guangxi province, China, and its taxonomic position was investigated using a polyphasic approach. The organism was found to have a range of chemical and morphological properties consistent with its classification in the genus . Phylogenetic analysis indicated that 16S rRNA gene sequence similarity between strain SCSIO 10427 and type strains of other recognized members of the genus was lower than 98.4 %. Furthermore, phenotypic characteristics revealed that the strain differed from the currently recognized species of the genus . Therefore, strain SCSIO 10427 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SCSIO 10427 ( = CCTCC AA 2011010 = DSM 45611).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.035402-0
2012-07-01
2019-10-14
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/7/1653.html?itemId=/content/journal/ijsem/10.1099/ijs.0.035402-0&mimeType=html&fmt=ahah

References

  1. Al-Tai A. M. , Ruan J. S. . ( 1994; ). Nocardiopsis halophila sp. nov., a new halophilic actinomycete isolated from soil. . Int J Syst Bacteriol 44:, 474–478. [CrossRef]
    [Google Scholar]
  2. Bernatchez H. , Lebreux E. . ( 1991; ). Nocardiopsis dassonvillei recovered from a lung biopsy and a possible cause of extrinsic allergic alveolitis. . Clin Microbiol Newsl 13:, 174–175. [CrossRef]
    [Google Scholar]
  3. Chen Y. G. , Cui X. L. , Kroppenstedt R. M. , Stackebrandt E. , Wen M. L. , Xu L. H. , Jiang C. L. . ( 2008; ). Nocardiopsis quinghaiensis sp. nov., isolated from saline soil in China. . Int J Syst Evol Microbiol 58:, 699–705. [CrossRef] [PubMed]
    [Google Scholar]
  4. Chen Y. G. , Wang Y. X. , Zhang Y. Q. , Tang S. K. , Liu Z. X. , Xiao H. D. , Xu L. H. , Cui X. L. , Li W. J. . ( 2009; ). Nocardiopsis litoralis sp. nov., a halophilic marine actinomycete isolated from a sea anemone. . Int J Syst Evol Microbiol 59:, 2708–2713. [CrossRef] [PubMed]
    [Google Scholar]
  5. Chun J. , Lee J. H. , Jung Y. , Kim M. , Kim S. , Kim B. K. , Lim Y. W. . ( 2007; ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. . Int J Syst Evol Microbiol 57:, 2259–2261. [CrossRef] [PubMed]
    [Google Scholar]
  6. Collins M. D. , Jones D. . ( 1980; ). Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4-diaminobutyric acid. . J Appl Bacteriol 48:, 459–470. [CrossRef]
    [Google Scholar]
  7. Collins M. D. , Pirouz T. , Goodfellow M. , Minnikin D. E. . ( 1977; ). Distribution of menaquinones in actinomycetes and Corynebacteria . . J Gen Microbiol 100:, 221–230.[PubMed] [CrossRef]
    [Google Scholar]
  8. Ding Z. G. , Li M. G. , Zhao J. Y. , Ren J. , Huang R. , Xie M. J. , Cui X. L. , Zhu H. J. , Wen M. L. . ( 2010; ). Naphthospironone A: an unprecedented and highly functionalized polycyclic metabolite from an alkaline mine waste extremophile. . Chemistry 16:, 3902–3905. [CrossRef] [PubMed]
    [Google Scholar]
  9. Dong X. Z. , Cai M. Y. . ( 2001; ). Manual of Systematics and Identification of General Bacteria. Beijing:: Science Press;.
    [Google Scholar]
  10. Engelhardt K. , Degnes K. F. , Kemmler M. , Bredholt H. , Fjaervik E. , Klinkenberg G. , Sletta H. , Ellingsen T. E. , Zotchev S. B. . ( 2010; ). Production of a new thiopeptide antibiotic, TP-1161, by a marine Nocardiopsis species. . Appl Environ Microbiol 76:, 4969–4976. [CrossRef] [PubMed]
    [Google Scholar]
  11. Fang C. Y. , Zhang J. L. , Pang H. C. , Li Y. Y. , Xin Y. H. , Zhang Y. B. . ( 2011; ). Nocardiopsis flavescens sp. nov., a novel actinomycete isolated from marine sediment in China. . Int J Syst Evol Microbiol 61:, 2640–2645. [CrossRef] [PubMed]
    [Google Scholar]
  12. Felsenstein J. . ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef] [PubMed]
    [Google Scholar]
  13. Felsenstein J. . ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  14. Fitch W. M. . ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20:, 406–416. [CrossRef]
    [Google Scholar]
  15. Gandhimathi R. , Seghal Kiran G. , Hema T. A. , Selvin J. , Rajeetha Raviji T. , Shanmughapriya S. . ( 2009; ). Production and characterization of lipopeptide biosurfactant by a sponge-associated marine actinomycetes Nocardiopsis alba MSA10. . Bioprocess Biosyst Eng 32:, 825–835. [CrossRef] [PubMed]
    [Google Scholar]
  16. Hamedi J. , Mohammadipanah F. , von Jan M. , Pötter G. , Schumann P. , Spröer C. , Klenk H. P. , Kroppenstedt R. M. . ( 2010; ). Nocardiopsis sinuspersici sp. nov., isolated from sandy rhizospheric soil. . Int J Syst Evol Microbiol 60:, 2346–2352. [CrossRef] [PubMed]
    [Google Scholar]
  17. Hamedi J. , Mohammadipanah F. , Pötter G. , Spröer C. , Schumann P. , Göker M. , Klenk H. P. . ( 2011; ). Nocardiopsis arvandica sp. nov., isolated from sandy soil. . Int J Syst Evol Microbiol 61:, 1189–1194. [CrossRef] [PubMed]
    [Google Scholar]
  18. Kämpfer P. , Busse H. J. , Rainey F. A. . ( 2002; ). Nocardiopsis compostus sp. nov., from the atmosphere of a composting facility. . Int J Syst Evol Microbiol 52:, 621–627.[PubMed]
    [Google Scholar]
  19. Kelly K. L. . ( 1964; ). Inter-Society Color Council – National Bureau of Standards Color-Name Charts Illustrated with Centroid Colors. Washington, DC:: US Government Printing Office;.
    [Google Scholar]
  20. Kim J. W. , Adachi H. , Shin-ya K. , Hayakawa Y. , Seto H. . ( 1997; ). Apoptolidin, a new apoptosis inducer in transformed cells from Nocardiopsis sp.. J Antibiot (Tokyo) 50:, 628–630. [CrossRef] [PubMed]
    [Google Scholar]
  21. Kroppenstedt R. M. . ( 1992; ). The genus Nocardiopsis . . In The Prokaryotes: A Handbook on the Biology of Bacteria: Ecophysiology, Isolation, Identification, Applications, , 2nd edn., pp. 1139–1156. Edited by Balows A. , Truper H. G. , Dworkin W. , Harder W. , Schleifer K. H. . . New York:: Springer;.
    [Google Scholar]
  22. Kroppenstedt R. M. , Evtushenko L. I. . ( 2002; ). The family Nocardiopsaceae . . In The Prokaryotes: a Handbook on the Biology of Bacteria, , 3rd edn., vol. 3, pp. 754–795. Edited by Dworkin M. , Falkow S. , Rosenberg E. , Schleifer K.-H. , Stackebrandt E. . . New York:: Springer;.
    [Google Scholar]
  23. Lechevalier M. P. , De Bièvre C. , Lechevalier H. A. . ( 1977; ). Chemotaxonomy of aerobic actinomycetes: phospholipid composition. . Biochem Syst Ecol 5:, 249–260. [CrossRef]
    [Google Scholar]
  24. Li M. G. , Li W. J. , Xu P. , Cui X. L. , Xu L. H. , Jiang C. L. . ( 2003; ). Nocardiopsis xinjiangensis sp. nov., a halophilic actinomycete isolated from a saline soil sample in China. . Int J Syst Evol Microbiol 53:, 317–321. [CrossRef] [PubMed]
    [Google Scholar]
  25. Li W. J. , Park D. J. , Tang S. K. , Wang D. , Lee J. C. , Xu L. H. , Kim C. J. , Jiang C. L. . ( 2004; ). Nocardiopsis salina sp. nov., a novel halophilic actinomycete isolated from saline soil in China. . Int J Syst Evol Microbiol 54:, 1805–1809. [CrossRef] [PubMed]
    [Google Scholar]
  26. Li W.-J. , Kroppenstedt R. M. , Wang D. , Tang S.-K. , Lee J.-C. , Park D.-J. , Kim C.-J. , Xu L.-H. , Jiang C.-L. . ( 2006; ). Five novel species of the genus Nocardiopsis isolated from hypersaline soils and emended description of Nocardiopsis salina Li et al. 2004. . Int J Syst Evol Microbiol 56:, 1089–1096. [CrossRef] [PubMed]
    [Google Scholar]
  27. Li Y. Q. , Li M. G. , Li W. , Zhao J. Y. , Ding Z. G. , Cui X. L. , Wen M. L. . ( 2007a; ). Griseusin D, a new pyranonaphthoquinone derivative from a alkaphilic Nocardiopsis sp.. J Antibiot (Tokyo) 60:, 757–761. [CrossRef] [PubMed]
    [Google Scholar]
  28. Li W. J. , Xu P. , Schumann P. , Zhang Y. Q. , Pukall R. , Xu L. H. , Stackebrandt E. , Jiang C. L. . ( 2007b; ). Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia . . Int J Syst Evol Microbiol 57:, 1424–1428. [CrossRef] [PubMed]
    [Google Scholar]
  29. Locci R. . ( 1989; ). Streptomycetes and related genera. . In Bergey’s Manual of Systematic Bacteriology, vol. 4, pp. 2451–2508. Edited by Williams S. T. , Sharpe M. E. , Holt J. G. . . Baltimore:: Williams & Wilkins Co;.
    [Google Scholar]
  30. Mesbah M. , Premachandran U. , Whitman W. B. . ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  31. Meyer J. . ( 1976; ). Nocardiopsis, a new genus of the order Actinomycetales . . Int J Syst Bacteriol 26:, 487–493. [CrossRef]
    [Google Scholar]
  32. Minnikin D. E. , Collins M. D. , Goodfellow M. . ( 1979; ). Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. . J Appl Bacteriol 47:, 87–95. [CrossRef]
    [Google Scholar]
  33. Peltola J. S. P. , Andersson M. A. , Kämpfer P. , Auling G. , Kroppenstedt R. M. , Busse H. J. , Salkinoja-Salonen M. S. , Rainey F. A. . ( 2001; ). Isolation of toxigenic Nocardiopsis strains from indoor environments and description of two new Nocardiopsis species, N. exhalans sp. nov. and N. umidischolae sp. nov.. Appl Environ Microbiol 67:, 4293–4304. [CrossRef] [PubMed]
    [Google Scholar]
  34. Qin S. , Li J. , Chen H. H. , Zhao G. Z. , Zhu W. Y. , Jiang C. L. , Xu L. H. , Li W. J. . ( 2009; ). Isolation, diversity, and antimicrobial activity of rare actinobacteria from medicinal plants of tropical rain forests in Xishuangbanna, China. . Appl Environ Microbiol 75:, 6176–6186. [CrossRef] [PubMed]
    [Google Scholar]
  35. Sabry S. A. , Ghanem N. B. , Abu-Ella G. A. , Schumann P. , Stackebrandt E. , Kroppenstedt R. M. . ( 2004; ). Nocardiopsis aegyptia sp. nov., isolated from marine sediment. . Int J Syst Evol Microbiol 54:, 453–456. [CrossRef] [PubMed]
    [Google Scholar]
  36. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  37. Schippers A. , Bosecker K. , Willscher S. , Spröer C. , Schumann P. , Kroppenstedt R. M. . ( 2002; ). Nocardiopsis metallicus sp. nov., a metal-leaching actinomycete isolated from an alkaline slag dump. . Int J Syst Evol Microbiol 52:, 2291–2295. [CrossRef] [PubMed]
    [Google Scholar]
  38. Shirling E. B. , Gottlieb D. . ( 1966; ). Methods for characterization of Streptomyces species. . Int J Syst Bacteriol 16:, 313–340. [CrossRef]
    [Google Scholar]
  39. Smibert R. M. , Krieg N. R. . ( 1994; ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P. , Murray R. G. E. , Wood W. A. , Krieg N. R. . . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  40. Stackebrandt E. , Ebers J. . ( 2006; ). Taxonomic parameters revisited: tarnished gold standards. . Microbiol Today 33:, 152–155.
    [Google Scholar]
  41. Staneck J. L. , Roberts G. D. . ( 1974; ). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. . Appl Microbiol 28:, 226–231.[PubMed]
    [Google Scholar]
  42. Sun H. H. , White C. B. , Dedinas J. , Cooper R. , Sedlock D. M. . ( 1991; ). Methylpendolmycin, an indolactam from a Nocardiopsis sp.. J Nat Prod 54:, 1440–1443. [CrossRef] [PubMed]
    [Google Scholar]
  43. Tamaoka J. , Katayama-Fujimura Y. , Kuraishi H. . ( 1983; ). Analysis of bacterial menaquinone mixtures by high performance liquid chromatography. . J Appl Bacteriol 54:, 31–36. [CrossRef]
    [Google Scholar]
  44. Tamura K. , Peterson D. , Peterson N. , Stecher G. , Nei M. , Kumar S. . ( 2011; ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef] [PubMed]
    [Google Scholar]
  45. Tang S. K. , Wang Y. , Chen Y. , Lou K. , Cao L. L. , Xu L. H. , Li W. J. . ( 2009; ). Zhihengliuella alba sp. nov., and emended description of the genus Zhihengliuella . . Int J Syst Evol Microbiol 59:, 2025–2031. [CrossRef] [PubMed]
    [Google Scholar]
  46. Thompson J. D. , Gibson T. J. , Plewniak F. , Jeanmougin F. , Higgins D. G. . ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef] [PubMed]
    [Google Scholar]
  47. Vasanthi V. , Hoti S. L. . ( 1992; ). Microbial flora in gut of Culex quinquefasciatus breeding in cess pits. . Southeast Asian J Trop Med Public Health 23:, 312–317.[PubMed]
    [Google Scholar]
  48. Williams S. T. , Goodfellow M. , Alderson G. . ( 1989; ). Genus Streptomyces Waksman and Henrici 1943, 339AL . . In Bergey’s Manual of Systematic Bacteriology, vol. 4, pp. 2452–2492. Edited by Williams S. T. , Sharpe M. E. , Holt J. G. . . Baltimore:: Williams & Wilkins;.
    [Google Scholar]
  49. Xu P. , Li W. J. , Tang S. K. , Zhang Y. Q. , Chen G. Z. , Chen H. H. , Xu L. H. , Jiang C. L. . ( 2005; ). Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family ‘Oxalobacteraceae’ isolated from China. . Int J Syst Evol Microbiol 55:, 1149–1153. [CrossRef] [PubMed]
    [Google Scholar]
  50. Yang R. , Zhang L. P. , Guo L. G. , Shi N. , Lu Z. , Zhang X. . ( 2008; ). Nocardiopsis valliformis sp. nov., an alkaliphilic actinomycete isolated from alkali lake soil in China. . Int J Syst Evol Microbiol 58:, 1542–1546. [CrossRef] [PubMed]
    [Google Scholar]
  51. Yassin A. F. , Rainey F. A. , Burghardt J. , Gierth D. , Ungerechts J. , Lux I. , Seifert P. , Bal C. , Schaal K. P. . ( 1997; ). Description of Nocardiopsis synnemataformans sp. nov., elevation of Nocardiopsis alba subsp. prasina to Nocardiopsis prasina comb. nov., and designation of Nocardiopsis antarctica and Nocardiopsis alborubida as later subjective synonyms of Nocardiopsis dassonvillei . . Int J Syst Bacteriol 47:, 983–988. [CrossRef] [PubMed]
    [Google Scholar]
  52. Yassin A. F. , Spröer C. , Hupfer H. , Siering C. , Klenk H. P. . ( 2009; ). Nocardiopsis potens sp. nov., isolated from household waste. . Int J Syst Evol Microbiol 59:, 2729–2733. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.035402-0
Loading
/content/journal/ijsem/10.1099/ijs.0.035402-0
Loading

Data & Media loading...

Supplementary material 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error