1887

Abstract

Bacterial isolates from oak trees in Spain and Britain, showing symptoms of bark canker and Acute Oak Decline (AOD), respectively, were examined by a polyphasic approach. Both 16S rRNA gene sequencing and multilocus sequence analysis (MLSA), based on partial sequences of , , and genes, revealed that the isolates were separated into two genetic groups according to their origin. Their closest phylogenetic relative was , the causal agent of drippy nut disease of oak, which clustered distant to the other species of the genus . MLSA data for species of the genera , , , , and confirmed the polyphyletic nature of the genus and indicated synonymy of and . DNA–DNA hybridization experiments confirmed this synonymy and also revealed DNA–DNA relatedness values of 58–73 % between the new oak isolates and . Phenotypic and/or chemotaxonomic methods allowed and the two genetic groups of new oak isolates to be discriminated from other recognized species of the genus and from members of the closely related genera , and . Based on the data obtained, the following taxonomic proposals are made: (1) reclassification of as the type species of a novel genus, gen. nov., as comb. nov. (type strain LMG 2724 = ATCC 29281 = CCUG 48867 = CFBP 3617 = CIP 105201 = DSM 4561 = ICMP 1845), (2) classification of the oak isolates as subsp. subsp. nov. (type strain LMG26264 = NCPPB 4490) and subsp. subsp. nov. (type strain LMG 26267 = NCPPB 4481) and leading to the automatic creation of subsp. subsp. nov. (type strain LMG 2724 = ATCC 29281), (3) emendation of the description of the genus , and (4) reclassification of as subsp. comb. nov. (type strain LMG 25992 = CFBP 2051), with the automatic creation of subsp. subsp. nov. (type strain LMG 25991 = CFBP 1269).

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2012-07-01
2019-10-21
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