1887

Abstract

A swarming and moderately halotolerant bacterium, designated strain A1-2, was isolated from the intestinal tract of the earthworm L. Cells were endospore-forming rods that were facultatively anaerobic, catalase-positive, oxidase-negative and motile by peritrichous flagella. The isolate grew optimally at 30 °C and pH 7.0, and could grow with up to 9 % (w/v) NaCl. Phylogenetic analysis of 16S rRNA gene sequences indicated that strain A1-2 belonged to the genus and exhibited 16S rRNA gene sequence similarities of 96.8, 96.0, 96.0, 96.4 and 96.7 % with LMG 21831, PT-45, BAC 1015, SMC 4352-1 and LMG 18435, respectively. DNA–DNA relatedness values between the isolate and the reference strains were ≤38.3 % The DNA G+C content of strain A1-2 was 38.5 mol%. The predominant menaquinone was MK-7 and the major polar lipids were diphosphatidylglycerol and phosphatidylethanolamine. The major cellular fatty acids were iso-C (51.5 %) and anteiso-C (29.6 %) and the cell-wall diamino acid was -diaminopimelic acid. On the basis of 16S rRNA gene sequence analysis and chemotaxonomic and phenotypic characteristics, it is concluded that strain A1-2 represents a novel species of the genus , for which we propose the name sp. nov. The type strain is A1-2 ( = KCCM 90092 = JCM 16993).

Funding
This study was supported by the:
  • Basic Science Research Program
  • National Research Foundation of Korea (NRF)
  • Ministry of Education, Science and Technology
  • Yonsei University Research Fund
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2012-09-01
2024-12-14
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References

  1. Albert R. A., Archambault J., Rosselló-Mora R., Tindall B. J., Matheny M. 2005; Bacillus acidicola sp. nov., a novel mesophilic, acidophilic species isolated from acidic Sphagnum peat bogs in Wisconsin. Int J Syst Evol Microbiol 55:2125–2130 [View Article][PubMed]
    [Google Scholar]
  2. Ash C., Farrow J. A. E., Wallbanks S., Collins M. D. 1991; Phylogenetic heterogeneity of the genus Bacillus revealed by comparative analysis of small-subunit-ribosomal RNA sequences. Lett Appl Microbiol 13:202–206 [View Article]
    [Google Scholar]
  3. Bae S. S., Lee J. H., Kim S. J. 2005; Bacillus alveayuensis sp. nov., a thermophilic bacterium isolated from deep-sea sediments of the Ayu Trough. Int J Syst Evol Microbiol 55:1211–1215 [View Article][PubMed]
    [Google Scholar]
  4. Breznak J. A., Costilow R. N. 1994; Physicochemical factors in growth. In Methods for General and Molecular Bacteriology pp. 137–154 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  5. Cohn F. 1872; Untersuchungen über Bakterien. Beitrage zur Biologie der Pflanzen 1:127–224 (in German)
    [Google Scholar]
  6. Edwards C. A., Fletcher K. E. 1988; Interactions between earthworms and microorganisms in organic-matter breakdown. Agric Ecosyst Environ 24:235–247 [View Article]
    [Google Scholar]
  7. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [View Article]
    [Google Scholar]
  8. Heyrman J., Vanparys B., Logan N. A., Balcaen A., Rodríguez-Díaz M., Felske A., De Vos P. 2004; Bacillus novalis sp. nov., Bacillus vireti sp. nov., Bacillus soli sp. nov., Bacillus bataviensis sp. nov. and Bacillus drentensis sp. nov., from the Drentse A grasslands. Int J Syst Evol Microbiol 54:47–57 [View Article][PubMed]
    [Google Scholar]
  9. Hong Y., Kim T. H., Na Y. E. 2001; Identity of two earthworms used in vermiculture and vermicomposting in Korea: Eisenia andrei and Perionyx excavatus . Kor J Soil Zool 17:185–190
    [Google Scholar]
  10. Hong S. W., Lee J. S., Chung K. S. 2011; Effect of enzyme producing microorganisms on the biomass of epigeic earthworms (Eisenia fetida) in vermicompost. Bioresour Technol 102:6344–6347 [View Article][PubMed]
    [Google Scholar]
  11. Kaiser D. 2007; Bacterial swarming: a re-examination of cell-movement patterns. Curr Biol 17:R561–R570 [View Article][PubMed]
    [Google Scholar]
  12. Kim H.-J., Shin K.-H., Cha C.-J., Hur H.-G. 2004; Analysis of aerobic and culturable bacterial community structures in earthworm (Eisenia fetida) intestine. Agric Chem Biotechnol 47:137–142
    [Google Scholar]
  13. Ko K. S., Oh W. S., Lee M. Y., Lee J. H., Lee H., Peck K. R., Lee N. Y., Song J.-H. 2006; Bacillus infantis sp. nov. and Bacillus idriensis sp. nov., isolated from a patient with neonatal sepsis. Int J Syst Evol Microbiol 56:2541–2544 [View Article][PubMed]
    [Google Scholar]
  14. Lane D. J., Pace B., Olsen G. J., Stahl D. A., Sogin M. L., Pace N. R. 1985; Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses. Proc Natl Acad Sci U S A 82:6955–6959 [View Article][PubMed]
    [Google Scholar]
  15. Logan N. A., De Vos P. 2009; Genus Bacillus Cohn 1872. In Bergey’s Manual of Systematic Bacteriology, 2nd edn. vol. 3 pp. 21–128 Edited by De Vos P., Garrity G. M., Jones D., Krieg N. R., Ludwig W., Rainey F. R., Schleifer K. H., Whitman W. B. New York: Springer;
    [Google Scholar]
  16. Logan N. A., Lebbe L., Verhelst A., Goris J., Forsyth G., Rodríguez-Díaz M., Heyndrickx M., De Vos P. 2004; Bacillus shackletonii sp. nov., from volcanic soil on Candlemas Island, South Sandwich archipelago. Int J Syst Evol Microbiol 54:373–376 [View Article][PubMed]
    [Google Scholar]
  17. Logan N. A., Popovitch T. J., Hoffmaster A. 2007; Bacillus and related genera. In Manual of Clinical Microbiology, 9th edn. vol. 1445–460 Edited by Murray P. R., Baron E. J., Jorgensen J. H., Pfaller M. A., Yolken R. H. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  18. Miller L. T. 1982; Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. J Clin Microbiol 16:584–586[PubMed]
    [Google Scholar]
  19. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [View Article]
    [Google Scholar]
  20. Nagel M., Andreesen J. R. 1991; Bacillus niacini sp. nov., a nicotinate-metabolizing mesophile isolated from soil. Int J Syst Bacteriol 41:134–139 [View Article]
    [Google Scholar]
  21. Nakagawa Y., Yamasato K. 1993; Phylogenetic diversity of the genus Cytophaga revealed by 16S rRNA sequencing and menaquinone analysis. J Gen Microbiol 139:1155–1161[PubMed] [CrossRef]
    [Google Scholar]
  22. Reichenbach H. 1981; Taxonomy of the gliding bacteria. Ann Rev Microbiol 35:339–364 [View Article][PubMed]
    [Google Scholar]
  23. Ruiz-García C., Quesada E., Martínez-Checa F., Llamas I., Urdaci M. C., Béjar V. 2005; Bacillus axarquiensis sp. nov. and Bacillus malacitensis sp. nov., isolated from river-mouth sediments in southern Spain. Int J Syst Evol Microbiol 55:1279–1285 [View Article][PubMed]
    [Google Scholar]
  24. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  25. Scheldeman P., Rodríguez-Díaz M., Goris J., Pil A., De Clerck E., Herman L., De Vos P., Logan N. A., Heyndrickx M. 2004; Bacillus farraginis sp. nov., Bacillus fortis sp. nov. and Bacillus fordii sp. nov., isolated at dairy farms. Int J Syst Evol Microbiol 54:1355–1364 [View Article][PubMed]
    [Google Scholar]
  26. Schönholzer F., Hahn D., Zeyer J. 1999; Origins and fate of fungi and bacteria in the gut of Lumbricus terrestris L. studied by image analysis. FEMS Microbiol Ecol 28:235–248 [View Article]
    [Google Scholar]
  27. Shin Y. K., Lee J. S., Kim H. J., Joo W. H., Lee J. D., Park Y. H. 1996; DNA base composition (G+C mol%) and its taxonomic implications. Kor J Life Sci 6:72–77
    [Google Scholar]
  28. Shin K.-H., Yi H., Chun J., Cha C.-J., Kim I. S., Hur H.-G. 2004; Analysis of the anaerobic bacterial community in the earthworm (Eisenia fetida) intestine. Agric Chem Biotechnol 47:147–152
    [Google Scholar]
  29. Smibert R. M., Krieg N. R. 1981; General characterization. In Manual of Methods for General Bacteriology pp. 409–443 Edited by Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. B. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  30. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [View Article]
    [Google Scholar]
  31. Staneck J. L., Roberts G. D. 1974; Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28:226–231 [View Article][PubMed]
    [Google Scholar]
  32. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [View Article][PubMed]
    [Google Scholar]
  33. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
    [Google Scholar]
  34. Tripathi G., Bhardwaj P. 2004; Decomposition of kitchen waste amended with cow manure using an epigeic species (Eisenia fetida) and an anecic species (Lampito mauritii). Bioresour Technol 92:215–218 [View Article][PubMed]
    [Google Scholar]
  35. Vaishampayan P., Probst A., Krishnamurthi S., Ghosh S., Osman S., McDowall A., Ruckmani A., Mayilraj S., Venkateswaran K. 2010; Bacillus horneckiae sp. nov., isolated from a spacecraft-assembly clean room. Int J Syst Evol Microbiol 60:1031–1037 [View Article][PubMed]
    [Google Scholar]
  36. Xue Y., Ventosa A., Wang X., Ren P., Zhou P., Ma Y. 2008; Bacillus aidingensis sp. nov., a moderately halophilic bacterium isolated from Ai-Ding salt lake in China. Int J Syst Evol Microbiol 58:2828–2832 [View Article][PubMed]
    [Google Scholar]
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