1887

Abstract

A bacterial strain named IGB-41 was isolated from a soil sample from an ant hill near Stuttgart, Germany. The strain was Gram-negative, rod-shaped, motile and facultatively anaerobic. Phylogenetic analysis based on 16S rRNA grouped the strain IGB-41 within the class into the family together with NBRC 103189, NBRC 103188 and KM-45 as the closest relatives with sequence similarities of 96.7, 96.6 and 96.1 %, respectively. The G+C content of the genomic DNA was determined to be 61.5 mol% and quinone analysis revealed Q-8 as the only detectable quinone. Major cellular fatty acids were identified as C, summed feature 3 (iso-C 2-OH and/or Cω7) and Cω7 . Strain IGB-41 was unique in harbouring phosphoaminolipids, aminolipids and glycoaminolipids when compared with NBRC 103189 in polar lipid analysis. On the basis of the physiological, phenotypic and genotypic characteristics of strain IGB-41, we suggest that the novel strain should be assigned to a new genus and novel species The type species of the genus is and the type strain is IGB-41 ( = DSM 23761 = CIP 110167).

Funding
This study was supported by the:
  • Bundesministerium für Bildung und Forschung (BMBF) (Award 0313712)
  • Neue Enzyme und Verfahren zur Herstellung von biobasierten Produkten durch Integration von biotechnologischen und chemischen Verfahren – BioSysPro
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2013-01-01
2024-10-14
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References

  1. Ashelford K. E., Chuzhanova N. A., Fry J. C., Jones A. J., Weightman A. J. ( 2006 ). New screening software shows that most recent large 16S rRNA gene clone libraries contain chimeras. . Appl Environ Microbiol 72, 57345741. [View Article] [PubMed]
    [Google Scholar]
  2. Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. (editors) ( 1987 ). Current Protocols in Molecular Biology. New York:: Green Publishing & Wiley Interscience;.
    [Google Scholar]
  3. Bast E. ( 2001 ). Mikrobiologische Methoden: Eine Einführung in grundlegende Arbeitstechniken. Heidelberg:: Spektrum Akademischer Verlag GmbH;.
    [Google Scholar]
  4. Campbell L. L., Williams O. B. ( 1951 ). A study of chitin-decomposing micro-organisms of marine origin. . J Gen Microbiol 5 (Suppl.), 894905. [View Article] [PubMed]
    [Google Scholar]
  5. Cashion P., Holder-Franklin M. A., McCully J., Franklin M. ( 1977 ). A rapid method for the base ratio determination of bacterial DNA. . Anal Biochem 81, 461466. [View Article] [PubMed]
    [Google Scholar]
  6. De Ley J., Cattoir H., Reynaerts A. ( 1970 ). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12, 133142. [View Article] [PubMed]
    [Google Scholar]
  7. Huß V. A. R., Festl H., Schleifer K. H. ( 1983 ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4, 184192. [View Article]
    [Google Scholar]
  8. Kuykendall L. D., Roy M. A., O'Neill J. J., Devine T. E. ( 1988 ). Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum . . Int J Syst Bacteriol 38, 358361. [View Article]
    [Google Scholar]
  9. Larkin M. A., Blackshields G., Brown N. P., Chenna R., McGettigan P. A., McWilliam H., Valentin F., Wallace I. M., Wilm A. & other authors ( 2007 ). clustal w and clustal_x version 2.0. . Bioinformatics 23, 29472948. [View Article] [PubMed]
    [Google Scholar]
  10. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S. & other authors ( 2004 ). arb: a software environment for sequence data. . Nucleic Acids Res 32, 13631371. [View Article] [PubMed]
    [Google Scholar]
  11. Mesbah M., Premachandran U., Whitman W. B. ( 1989 ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39, 159167. [View Article]
    [Google Scholar]
  12. Miller L. T. ( 1982 ). Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. . J Clin Microbiol 16, 584586.[PubMed]
    [Google Scholar]
  13. Muramatsu Y., Suzuki K., Nakagawa Y. ( 2010 ). Silvimonas iriomotensis sp. nov. and Silvimonas amylolytica sp. nov., new members of the class Betaproteobacteria isolated from the subtropical zone in Japan. . Int J Syst Evol Microbiol 60, 174178. [View Article] [PubMed]
    [Google Scholar]
  14. Patil R. S., Ghormade V., Deshpande M. V. ( 2000 ). Chitinolytic enzymes: an exploration. . Enzyme Microb Technol 26, 473483. [View Article] [PubMed]
    [Google Scholar]
  15. Reddy C. A., Beveridge T. J., Breznak J. A., Snyder L., Schmidt T. M., Marzluf G. A. ( 2007 ). Methods for General and Molecular Microbiology. Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  16. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  17. Sheu S. Y., Chiu T. F., Chou J. H., Sheu D. S., Arun A. B., Young C. C., Chen C. A., Wang J. T., Chen W. M. ( 2009 ). Andreprevotia lacus sp. nov., isolated from a fish-culture pond. . Int J Syst Evol Microbiol 59, 24822485. [View Article] [PubMed]
    [Google Scholar]
  18. Stackebrandt E., Goebel B. M. ( 1994 ). Taxonomic note: A place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44, 846849. [View Article]
    [Google Scholar]
  19. Stamatakis A. ( 2006 ). RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. . Bioinformatics 22, 26882690. [View Article] [PubMed]
    [Google Scholar]
  20. Tamaoka J., Komagata K. ( 1984 ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25, 125128. [View Article]
    [Google Scholar]
  21. Tindall B. J. ( 1990a ). A comparative study of the lipid composition of Halobacterium sacharovorum from various sources. . Syst Appl Microbiol 13, 128130. [View Article]
    [Google Scholar]
  22. Tindall B. J. ( 1990b ). Lipid composition of Halobacterium lacusprofundi . . FEMS Microbiol Lett 66, 199202. [View Article]
    [Google Scholar]
  23. Tindall B. J., Sikorski J., Smibert R. M., Kreig N. R. ( 2007 ). Phenotypic characterization and the principles of comparative systematics. . In Methods for General and Molecular Microbiology, , 3rd edn.. pp. 330393. Edited by Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G. A., Schmidt T. M., Snyder L. . Washington, DC, USA:: American Society for Microbiology;.
    [Google Scholar]
  24. Tindall B. J., Rosselló-Móra R., Busse H. J., Ludwig W., Kämpfer P. ( 2010 ). Notes on the characterization of prokaryote strains for taxonomic purposes. . Int J Syst Evol Microbiol 60, 249266. [View Article] [PubMed]
    [Google Scholar]
  25. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. & other authors ( 1987 ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463464. [View Article]
    [Google Scholar]
  26. Weon H.-Y., Kim B.-Y., Yoo S.-H., Joa J.-H., Kwon S.-W., Kim W.-G. ( 2007 ). Andreprevotia chitinilytica gen. nov., sp. nov., isolated from forest soil from Halla Mountain, Jeju Island, Korea. . Int J Syst Evol Microbiol 57, 15721575. [View Article] [PubMed]
    [Google Scholar]
  27. Yang H.-C., Im W.-T., An D.-S., Park W. S., Kim I. S., Lee S.-T. ( 2005 ). Silvimonas terrae gen. nov., sp. nov., a novel chitin-degrading facultative anaerobe belonging to the ‘Betaproteobacteria’. . Int J Syst Evol Microbiol 55, 23292332. [View Article] [PubMed]
    [Google Scholar]
  28. Yoon J. H., Choi J. H., Kang S. J., Choi N. S., Lee J. S., Song J. J. ( 2010 ). Jeongeupia naejangsanensis gen. nov., sp. nov., a cellulose-degrading bacterium isolated from forest soil from Naejang Mountain in Korea. . Int J Syst Evol Microbiol 60, 615619. [View Article] [PubMed]
    [Google Scholar]
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