1887

Abstract

A bacterial strain named IGB-41 was isolated from a soil sample from an ant hill near Stuttgart, Germany. The strain was Gram-negative, rod-shaped, motile and facultatively anaerobic. Phylogenetic analysis based on 16S rRNA grouped the strain IGB-41 within the class into the family together with NBRC 103189, NBRC 103188 and KM-45 as the closest relatives with sequence similarities of 96.7, 96.6 and 96.1 %, respectively. The G+C content of the genomic DNA was determined to be 61.5 mol% and quinone analysis revealed Q-8 as the only detectable quinone. Major cellular fatty acids were identified as C, summed feature 3 (iso-C 2-OH and/or Cω7) and Cω7 . Strain IGB-41 was unique in harbouring phosphoaminolipids, aminolipids and glycoaminolipids when compared with NBRC 103189 in polar lipid analysis. On the basis of the physiological, phenotypic and genotypic characteristics of strain IGB-41, we suggest that the novel strain should be assigned to a new genus and novel species The type species of the genus is and the type strain is IGB-41 ( = DSM 23761 = CIP 110167).

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2013-01-01
2019-12-08
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References

  1. Ashelford K. E., Chuzhanova N. A., Fry J. C., Jones A. J., Weightman A. J.. ( 2006;). New screening software shows that most recent large 16S rRNA gene clone libraries contain chimeras. . Appl Environ Microbiol 72:, 5734–5741. [CrossRef][PubMed]
    [Google Scholar]
  2. Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K.. (editors) ( 1987;). Current Protocols in Molecular Biology. New York:: Green Publishing & Wiley Interscience;.
    [Google Scholar]
  3. Bast E.. ( 2001;). Mikrobiologische Methoden: Eine Einführung in grundlegende Arbeitstechniken. Heidelberg:: Spektrum Akademischer Verlag GmbH;.
    [Google Scholar]
  4. Campbell L. L., Williams O. B.. ( 1951;). A study of chitin-decomposing micro-organisms of marine origin. . J Gen Microbiol 5: (Suppl.), 894–905. [CrossRef][PubMed]
    [Google Scholar]
  5. Cashion P., Holder-Franklin M. A., McCully J., Franklin M.. ( 1977;). A rapid method for the base ratio determination of bacterial DNA. . Anal Biochem 81:, 461–466. [CrossRef][PubMed]
    [Google Scholar]
  6. De Ley J., Cattoir H., Reynaerts A.. ( 1970;). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12:, 133–142. [CrossRef][PubMed]
    [Google Scholar]
  7. Huß V. A. R., Festl H., Schleifer K. H.. ( 1983;). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4:, 184–192. [CrossRef]
    [Google Scholar]
  8. Kuykendall L. D., Roy M. A., O'Neill J. J., Devine T. E.. ( 1988;). Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum. . Int J Syst Bacteriol 38:, 358–361. [CrossRef]
    [Google Scholar]
  9. Larkin M. A., Blackshields G., Brown N. P., Chenna R., McGettigan P. A., McWilliam H., Valentin F., Wallace I. M., Wilm A.. & other authors ( 2007;). clustal w and clustal_x version 2.0. . Bioinformatics 23:, 2947–2948. [CrossRef][PubMed]
    [Google Scholar]
  10. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S.. & other authors ( 2004;). arb: a software environment for sequence data. . Nucleic Acids Res 32:, 1363–1371. [CrossRef][PubMed]
    [Google Scholar]
  11. Mesbah M., Premachandran U., Whitman W. B.. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  12. Miller L. T.. ( 1982;). Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. . J Clin Microbiol 16:, 584–586.[PubMed]
    [Google Scholar]
  13. Muramatsu Y., Suzuki K., Nakagawa Y.. ( 2010;). Silvimonas iriomotensis sp. nov. and Silvimonas amylolytica sp. nov., new members of the class Betaproteobacteria isolated from the subtropical zone in Japan. . Int J Syst Evol Microbiol 60:, 174–178. [CrossRef][PubMed]
    [Google Scholar]
  14. Patil R. S., Ghormade V., Deshpande M. V.. ( 2000;). Chitinolytic enzymes: an exploration. . Enzyme Microb Technol 26:, 473–483. [CrossRef][PubMed]
    [Google Scholar]
  15. Reddy C. A., Beveridge T. J., Breznak J. A., Snyder L., Schmidt T. M., Marzluf G. A.. ( 2007;). Methods for General and Molecular Microbiology. Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  16. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  17. Sheu S. Y., Chiu T. F., Chou J. H., Sheu D. S., Arun A. B., Young C. C., Chen C. A., Wang J. T., Chen W. M.. ( 2009;). Andreprevotia lacus sp. nov., isolated from a fish-culture pond. . Int J Syst Evol Microbiol 59:, 2482–2485. [CrossRef][PubMed]
    [Google Scholar]
  18. Stackebrandt E., Goebel B. M.. ( 1994;). Taxonomic note: A place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44:, 846–849. [CrossRef]
    [Google Scholar]
  19. Stamatakis A.. ( 2006;). RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. . Bioinformatics 22:, 2688–2690. [CrossRef][PubMed]
    [Google Scholar]
  20. Tamaoka J., Komagata K.. ( 1984;). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25:, 125–128. [CrossRef]
    [Google Scholar]
  21. Tindall B. J.. ( 1990a;). A comparative study of the lipid composition of Halobacterium sacharovorum from various sources. . Syst Appl Microbiol 13:, 128–130. [CrossRef]
    [Google Scholar]
  22. Tindall B. J.. ( 1990b;). Lipid composition of Halobacterium lacusprofundi. . FEMS Microbiol Lett 66:, 199–202. [CrossRef]
    [Google Scholar]
  23. Tindall B. J., Sikorski J., Smibert R. M., Kreig N. R.. ( 2007;). Phenotypic characterization and the principles of comparative systematics. . In Methods for General and Molecular Microbiology, , 3rd edn.. pp. 330–393. Edited by Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G. A., Schmidt T. M., Snyder L... Washington, DC, USA:: American Society for Microbiology;.
    [Google Scholar]
  24. Tindall B. J., Rosselló-Móra R., Busse H. J., Ludwig W., Kämpfer P.. ( 2010;). Notes on the characterization of prokaryote strains for taxonomic purposes. . Int J Syst Evol Microbiol 60:, 249–266. [CrossRef][PubMed]
    [Google Scholar]
  25. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E.. & other authors ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
  26. Weon H.-Y., Kim B.-Y., Yoo S.-H., Joa J.-H., Kwon S.-W., Kim W.-G.. ( 2007;). Andreprevotia chitinilytica gen. nov., sp. nov., isolated from forest soil from Halla Mountain, Jeju Island, Korea. . Int J Syst Evol Microbiol 57:, 1572–1575. [CrossRef][PubMed]
    [Google Scholar]
  27. Yang H.-C., Im W.-T., An D.-S., Park W. S., Kim I. S., Lee S.-T.. ( 2005;). Silvimonas terrae gen. nov., sp. nov., a novel chitin-degrading facultative anaerobe belonging to the ‘Betaproteobacteria’. . Int J Syst Evol Microbiol 55:, 2329–2332. [CrossRef][PubMed]
    [Google Scholar]
  28. Yoon J. H., Choi J. H., Kang S. J., Choi N. S., Lee J. S., Song J. J.. ( 2010;). Jeongeupia naejangsanensis gen. nov., sp. nov., a cellulose-degrading bacterium isolated from forest soil from Naejang Mountain in Korea. . Int J Syst Evol Microbiol 60:, 615–619. [CrossRef][PubMed]
    [Google Scholar]
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