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Volume 62, Issue Pt_7

gen. nov., sp. nov., an alkaliphilic, lithoautotrophic homoacetogen from a soda lake Free

Abstract

The first alkaliphilic obligately anaerobic hydrogenotrophic homoacetogenic bacterium, strain Z-7100, was isolated from sediments of the soda-depositing soda lake Tanatar III (Altay, Russia). Cells were thin, flexible rods, motile, Gram-negative and spore-forming. The organism was an obligate alkaliphile, growing at pH 8.5 to 10.5, with optimum growth at pH 8.8–9.3, and it grew in soda brines containing 1.9–4.7 M total Na (optimum at 2.8–3.3 M). It exhibited an obligate dependence upon sodium carbonate but not upon chloride ions with an NaCl range for growth of 0–14 % (w/v) and an optimum at 7.0–8.5 % (w/v). The isolate was mesophilic and grew at temperatures from 25 to 45 °C, with an optimum at 40 °C. An H+CO mixture, ethanol, pyruvate and lactate were utilized with the formation of acetate as the sole metabolic product. Carbohydrates and amino acids did not support growth. The isolate had a respiratory type of metabolism, reducing , SeO or anthraquinone-2,6-disulfonate (as electron acceptors with ethanol as an electron donor). It was able to grow chemolithotrophically on H+CO in medium supplemented with a vitamin solution only. The major cellular fatty acids were the saturated fatty acids anteiso-C, C and C and the aldehydes C, C and anteiso-C. The DNA G+C content of the isolate was 32.0 mol%. 16S rRNA gene sequence analysis showed that strain Z-7100 is a member of the order and represents a new branch within the family , clustering with the type strain of (92.9 % gene sequence similarity). On the basis of its physiological characteristics and phylogenetic position, the isolate is considered to represent a novel species in a new genus within the family The name gen. nov., sp. nov. is proposed. The type strain of the type species is Z-7100 ( = DSM 24880 = VKM B-2667).

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Funding
This study was supported by the:
  • Presidium RAS ‘The origin of life and evolution of geo-biological systems’ (Award #02.740.11.0023)
© 2012 IUMS
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2012-07-01
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Fuchsiella alkaliacetigena gen. nov., sp. nov., an alkaliphilic, lithoautotrophic homoacetogen from a soda lake
Int J Syst Evol Microbiol 62, 1666 (2012); https://doi.org/10.1099/ijs.0.034363-0
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