1887

Abstract

Three apiculate yeast strains, EJ7M09, GJ5M15 and GJ15M04, isolated from mushrooms in Taiwan were found to represent a novel species of the genus . The phylogenetically closest relative of this novel species is , but the type strain of differed by 4.6 % divergence (25 substitutions; 5 gaps) in the sequence of the D1/D2 domain of the large subunit rRNA gene. This difference clearly suggests that the three strains represent a distinct species. As none of the strains that were examined in this study produced ascospores or exhibited conjugation on common sporulation medium either alone or in a pairwise mixture, this species could be considered as an anamorphic member of the genus , and a novel species, sp. nov., is proposed, with EJ7M09 ( = BCRC 23182 = CBS 11434) as the type strain.

Funding
This study was supported by the:
  • , National Science Council, Executive Yuan, Taiwan , (Award NSC 97-2621-B-134-001-MY3)
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2012-06-01
2021-01-26
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References

  1. Boekhout T., Kurtzman C. P., O’Donnell K., Smith M. T. 1994; Phylogeny of the yeast genera Hanseniaspora (anamorph Kloeckera), Dekkera (anamorph Brettanomyces), and Eeniella as inferred from partial 26S ribosomal DNA nucleotide sequences. Int J Syst Bacteriol 44:781–786 [CrossRef][PubMed]
    [Google Scholar]
  2. Cadez N., Raspor P., de Cock A. W. A. M., Boekhout T., Smith M. Th. 2002; Molecular identification and genetic diversity within species of the genera Hanseniaspora and Kloeckera . FEMS Yeast Res 1:279–289[PubMed]
    [Google Scholar]
  3. Cadez N., Poot G. A., Raspor P., Smith M. T. 2003; Hanseniaspora meyeri sp. nov., Hanseniaspora clermontiae sp. nov., Hanseniaspora lachancei sp. nov. and Hanseniaspora opuntiae sp. nov., novel apiculate yeast species. Int J Syst Evol Microbiol 53:1671–1680 [CrossRef][PubMed]
    [Google Scholar]
  4. Cadez N., Raspor P., Smith M. T. 2006; Phylogenetic placement of Hanseniaspora-Kloeckera species using multigene sequence analysis with taxonomic implications: descriptions of Hanseniaspora pseudoguilliermondii sp. nov. and Hanseniaspora occidentalis var. citrica var. nov.. Int J Syst Evol Microbiol 56:1157–1165 [CrossRef][PubMed]
    [Google Scholar]
  5. Esteve-Zarzoso B., Peris-Torán M. J., Ramón D., Quero A. 2001; Molecular characterisation of Hanseniaspora species. Antonie van Leeuwenhoek 80:85–92 [CrossRef][PubMed]
    [Google Scholar]
  6. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  7. Hsieh C. W., Huang L. Y., Tschen E. F., Chang C. F., Lee C. F. 2010; Five novel anamorphic, ascomycetous yeast species associated with mushrooms and soil. FEMS Yeast Res 10:948–956 [CrossRef][PubMed]
    [Google Scholar]
  8. Janke A. 1923; Zur Nomenklatur der “Saccharomyces apiculatus”-Formen. Zentralbl Bakteriol Protozool Parasitenkd Infektionskr Abt II 56:310–311 (in German)
    [Google Scholar]
  9. Jindamorakot S., Ninomiya S., Limtong S., Yongmanitchai W., Tuntirungkij M., Potacharoen W., Tanaka K., Kawasaki H., Nakase T. 2009; Three new species of bipolar budding yeasts of the genus Hanseniaspora and its anamorph Kloeckera isolated in Thailand. FEMS Yeast Res 9:1327–1337 [CrossRef][PubMed]
    [Google Scholar]
  10. Kurtzman C. P., Robnett C. J. 1998; Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 73:331–371 [CrossRef][PubMed]
    [Google Scholar]
  11. Kurtzman C. P., Robnett C. J. 2003; Phylogenetic relationships among yeasts of the ‘Saccharomyces complex’ determined from multigene sequence analyses. FEMS Yeast Res 3:417–432 [CrossRef][PubMed]
    [Google Scholar]
  12. Meyer S. A., Smith M. Th., Simione F. P. Jr 1978; Systematics of Hanseniaspora Zikes and Kloeckera Janke. Antonie van Leeuwenhoek 44:79–96 [CrossRef][PubMed]
    [Google Scholar]
  13. Nakase T., Komagata K. 1970; Significance of DNA base composition in the classification of yeast genera Hanseniaspora and Kloeckera . J Gen Appl Microbiol 16:241–250 [CrossRef]
    [Google Scholar]
  14. Reess M. 1870 Botanische Untersuchungen über die Alkoholgährungspilze Leipzig: A. Felix;
    [Google Scholar]
  15. Smith M. Th. 1998a; Hanseniaspora Zikes. In The Yeasts, a Taxonomic Study, 4th edn. pp. 214–220 Edited by Kurtzman C. P., Fell J. W. Amsterdam: Elsevier; [CrossRef]
    [Google Scholar]
  16. Smith M. Th. 1998b; Kloeckera Janke. In The Yeasts, a Taxonomic Study, 4th edn. pp. 580–581 Edited by Kurtzman C. P., Fell J. W. Amsterdam: Elsevier; [CrossRef]
    [Google Scholar]
  17. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599[PubMed]
    [Google Scholar]
  18. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef][PubMed]
    [Google Scholar]
  19. Yamada Y., Maeda K., Banno I. 1992; The phylogenetic relationships of the Q6-equipped species in the teleomorphic apiculate yeast genera Hanseniaspora, Nadsonia, and Saccharomycodes based on the partial sequences of 18S and 26S ribosomal ribonucleic acids. J Gen Appl Microbiol 38:585–596 [CrossRef]
    [Google Scholar]
  20. Yarrow D. 1998; Methods for the isolation, maintenance and identification of yeasts. In The Yeasts, a Taxonomic Study, 4th edn. pp. 77–100 Edited by Kurtzman C. P., Fell J. W. Amsterdam: Elsevier; [CrossRef]
    [Google Scholar]
  21. Zikes H. 1911; Zur Nomenclaturfrage der Apiculatushefe. Zentralbl Bakteriol Parasitenkd Abt II 30:145–149 (in German)
    [Google Scholar]
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