1887

Abstract

A Gram-stain-positive actinobacterium, strain PM267, was isolated from a marine sediment sample in the Mediterranean Sea. The novel strain produced extensively branched substrate and aerial hyphae that carried spiral spore chains. Substrate and aerial mycelia were cream–white and white, respectively. Diffusible pigments were not observed. 16S rRNA gene sequence analysis revealed that strain PM267 belonged to the genus and shared a gene sequence similarity of 97.1 % with YIM 63135 and JCM 3070. Values <97 % were obtained with other sequences representing members of the genus . The cell wall peptidoglycan contained diaminopimelic acid. MK-9(H) was the major menaquinone. The phospholipid pattern included phosphatidylethanolamine as diagnostic lipid (type II). Major fatty acids found were iso- and anteiso- fatty acids. The G+C content of the DNA was 71.2 mol%. The strain was halotolerant and was able to grow in the presence of 9 % (w/v) NaCl (with an optimum of 2 %). On the basis of these results and additional physiological data obtained in the present study, strain PM267 represents a novel species within the genus for which the name sp. nov. is proposed (type strain PM267  = CECT 7841  = DSM 42032).

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2012-05-01
2019-10-14
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