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Abstract
A Micromonospora -like strain, RtIII47T, was isolated from a mangrove swamp in Sanya, Hainan Province, China. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the strain had a close association with the genus Verrucosispora and shared the highest sequence similarity with Verrucosispora lutea YIM 013T (98.0 %). The strain also showed high 16S rRNA gene sequence similarities to Micromonospora olivasterospora DSM 43868T (97.9 %), Plantactinospora mayteni YIM 61359T (97.9 %), Salinispora tropica CNB-440T (97.8 %), Micromonospora peucetia DSM 43363T (97.7 %), Micromonospora auratinigra TT1-11T (97.7 %), Verrucosispora sediminis CGMCC 4.3550T (97.6 %) and Salinispora arenicola CNH-643T (97.5 %). Phylogenetic analysis based on the gyrB gene sequence supported the conclusion that strain RtIII47T should be assigned to the genus Verrucosispora . DNA–DNA relatedness between strain RtIII47T and the most closely related type strain, V. lutea YIM 013T, was less than 40 %. Chemotaxonomic results confirmed the taxonomic position of the isolate in the genus Verrucosispora , and revealed differences at the species level in polar lipids, whole-cell sugars and DNA G+C content. A combination of physiological and biochemical tests also distinguished this strain from other Verrucosispora species. Based on genotypic and phenotypic observations, strain RtIII47T ( = CGMCC 4.5826T = NBRC 106684T) is proposed as the type strain of a novel species, Verrucosispora qiuiae sp. nov. An emended description of the genus Verrucosispora is also provided.
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Funding
- Knowledge Innovation Program of the Chinese Academy of Sciences (Award KSCX2-EW-G-12B/ KSCX2-EW-J-6)