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Abstract

A novel starch-degrading myxobacterium designated NOSO-4 (ew rganism of the strain 4) was isolated in 1995 from a soil sample containing plant residues, collected in Lucknow, Uttar Pradesh, India. The novel bacterium shows typical myxobacterial characteristics such as Gram-negative, rod-shaped vegetative cells, swarming colonies, fruiting body-like aggregates and bacteriolytic activity. The strain is mesophilic, strictly aerobic and chemoheterotrophic. Based on 16S rRNA gene sequences, NOSO-4 shows highest similarity (96.2 %) with the unidentified bacterial strain O29 (accession no. FN554397), isolated from leek () rhizosphere, and to the myxobacteria (88.9 %) and (88.5 %). Major fatty acids are C 2-OH, Cω6 (arachidonic acid), and the straight-chain fatty acids C, C and C. The genomic DNA G+C content of the novel isolate is 66.8 mol%. It is proposed that strain NOSO-4 represents a novel species in a new genus, i.e. gen. nov., sp. nov., but also belongs to a new family, fam. nov. The type strain of the type species, sp. nov., is NOSO-4 ( = DSM 53668 = NCCB 100362).

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2012-05-01
2020-01-18
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References

  1. Fudou R. , Jojima Y. , Iizuka T. , Yamanaka S. . ( 2002; ). Haliangium ochraceum gen. nov., sp. nov. and Haliangium tepidum sp. nov.: novel moderately halophilic myxobacteria isolated from coastal saline environments. . J Gen Appl Microbiol 48:, 109–115. [CrossRef] [PubMed]
    [Google Scholar]
  2. Garcia R. , Krug D. , Müller R. . ( 2009a; ). Discovering natural products from myxobacteria with emphasis on rare producer strains in combination with improved analytical methods. . Methods Enzymol 458, 59–91. [CrossRef]
    [Google Scholar]
  3. Garcia R. O. , Reichenbach H. , Ring M. W. , Müller R. . ( 2009b; ). Phaselicystis flava gen. nov., sp. nov., an arachidonic acid-containing soil myxobacterium, and the description of Phaselicystidaceae fam. nov.. Int J Syst Evol Microbiol 59:, 1524–1530. [CrossRef] [PubMed]
    [Google Scholar]
  4. Garcia R. , Gerth K. , Stadler M. , Dogma I. J. Jr , Müller R. . ( 2010; ). Expanded phylogeny of myxobacteria and evidence for cultivation of the ‘unculturables’. . Mol Phylogenet Evol 57:, 878–887. [CrossRef] [PubMed]
    [Google Scholar]
  5. Garcia R. , Pistorius D. , Stadler M. , Müller R. . ( 2011; ). Fatty acid-related phylogeny of myxobacteria as an approach to discover polyunsaturated omega-3/6 fatty acids. . J Bacteriol 193:, 1930–1942. [CrossRef] [PubMed]
    [Google Scholar]
  6. Gerth K. , Müller R. . ( 2005; ). Moderately thermophilic myxobacteria: novel potential for the production of natural products isolation and characterization. . Environ Microbiol 7:, 874–880. [CrossRef] [PubMed]
    [Google Scholar]
  7. Iizuka T. , Jojima Y. , Fudou R. , Hiraishi A. , Ahn J.-W. , Yamanaka S. . ( 2003; ). Plesiocystis pacifica gen. nov., sp. nov., a marine myxobacterium that contains dihydrogenated menaquinone, isolated from the Pacific coasts of Japan. . Int J Syst Evol Microbiol 53:, 189–195. [CrossRef] [PubMed]
    [Google Scholar]
  8. Irschik H. , Reichenbach H. . ( 1985; ). An unusual pattern of carbohydrate utilization in Corallococcus (Myxococcus) coralloides (Myxobacterales). . Arch Microbiol 142:, 40–44. [CrossRef]
    [Google Scholar]
  9. Jansen R. , Nowak A. , Kunze B. , Reichenbach H. , Hofle G. . ( 1995; ). Four new carotenoids from Polyangium fumosum (myxobacteria): 3,3′,4,4′-tetradehydro-l,1′,2,2′-tetrahydro-l,1′-dihydroxy-ψ,ψ-carotene (di-O-demethylspirilloxanthin), its β-glucoside and glucoside fatty acid esters. . Liebigs Ann 1995:, 873–876. [CrossRef]
    [Google Scholar]
  10. Jukes T. H. , Cantor C. R. . ( 1969; ). Evolution of protein molecules. . In Mammalian Protein Metabolism, pp. 21–123. Edited by Munro H. N. . . New York:: Academic Press;.
    [Google Scholar]
  11. Kovacs N. . ( 1956; ). Identification of Pseudomonas pyocyanea by the oxidase reaction. . Nature 178:, 703. [CrossRef] [PubMed]
    [Google Scholar]
  12. Lane D. J. . ( 1991; ). 16S/23S rDNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by Stackebrandt E. , Goodfellow M. . . Chichester:: Wiley;.
    [Google Scholar]
  13. Li G. , Shimelis O. , Zhou X. , Giese R. W. . ( 2003; ). Scaled-down nuclease P1 for scaled-up DNA digestion. . Biotechniques 34:, 908–909.[PubMed]
    [Google Scholar]
  14. Ludwig W. , Strunk O. , Westram R. , Richter L. , Meier H. , Yadhukumar , Buchner A. , Lai T. , Steppi S. . & other authors ( 2004; ). ARB: a software environment for sequence data. . Nucleic Acids Res 32:, 1363–1371. [CrossRef] [PubMed]
    [Google Scholar]
  15. Madigan M. T. , Martinko J. M. , Parker J. . ( 2001; ). Brock Mikrobiologie (translation of the 9th edn). Berlin: Spektrum Akademischer Verlag.
  16. McCurdy H. D. . ( 1969; ). Studies on the taxonomy of the Myxobacterales. I. Record of Canadian isolates and survey of methods. . Can J Microbiol 15:, 1453–1461. [CrossRef] [PubMed]
    [Google Scholar]
  17. Müller R. , Gerth K. . ( 2006; ). Development of simple media which allow investigations into the global regulation of chivosazol biosynthesis with Sorangium cellulosum So ce56. . J Biotechnol 121:, 192–200. [CrossRef] [PubMed]
    [Google Scholar]
  18. Pospiech A. , Neumann B. . ( 1995; ). A versatile quick-prep of genomic DNA from gram-positive bacteria. . Trends Genet 11:, 217–218. [CrossRef] [PubMed]
    [Google Scholar]
  19. Reichenbach H. . ( 2005; ). Order VIII. Myxococcales Tchan, Pochon and Prévot 1948, 398AL . . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 2, pp. 1059–1072. Edited by Brenner D. J. , Krieg N. R. , Staley J. T. , Garrity G. M. . . New York:: Springer;.
    [Google Scholar]
  20. Reichenbach H. . ( 2006; ). The genus Lysobacter . . In The Prokaryotes: a Handbook on the Biology of Bacteria, , 3rd edn., vol. 6, pp. 939–957. Edited by Dworkin M. , Falkow S. , Rosenberg E. , Schleifer K. H. , Stackebrandt E. . . New York:: Springer;.
    [Google Scholar]
  21. Reichenbach H. , Dworkin M. . ( 1992; ). The myxobacteria. . In The Prokaryotes, , 2nd edn., pp. 3416–3487. Edited by Balows A. , Trüper H. G. , Dworkin M. , Harder W. , Schleifer K.-H. . . Berlin:: Springer-Verlag;.[CrossRef]
    [Google Scholar]
  22. Reichenbach H. , Höfle G. . ( 1999; ). Myxobacteria as producers of secondary metabolites. . In Drug Discovery from Nature, pp. 149–179. Edited by Grabley S. , Thiericke R. . . Berlin, Heidelberg:: Springer-Verlag;.
    [Google Scholar]
  23. Reichenbach H. , Lang E. , Schumann P. , Spröer C. . ( 2006; ). Byssovorax cruenta gen. nov., sp. nov., nom. rev., a cellulose-degrading myxobacterium: rediscovery of ‘Myxococcus cruentus’ Thaxter 1897. . Int J Syst Evol Microbiol 56:, 2357–2363. [CrossRef] [PubMed]
    [Google Scholar]
  24. Rosenberg E. , Varon M. . ( 1984; ). Antibiotics and lytic enzymes. . In Myxobacteria: Development and Cell Interactions, pp. 109–127. Edited by Rosenberg E. . . New York:: Springer-Verlag;.
    [Google Scholar]
  25. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  26. Sanford R. A. , Cole J. R. , Tiedje J. M. . ( 2002; ). Characterization and description of Anaeromyxobacter dehalogenans gen. nov., sp. nov., an aryl-halorespiring facultative anaerobic myxobacterium. . Appl Environ Microbiol 68:, 893–900. [CrossRef] [PubMed]
    [Google Scholar]
  27. Schlegel H. G. . ( 1992; ). Allgemeine Mikrobiologie, , 7th edn.. Stuttgart:: Thieme Verlag;.
    [Google Scholar]
  28. Shimelis O. , Giese R. W. . ( 2006; ). Nuclease P1 digestion/high-performance liquid chromatography, a practical method for DNA quantitation. . J Chromatogr A 1117:, 132–136. [CrossRef] [PubMed]
    [Google Scholar]
  29. Shimkets L. J. , Woese C. R. . ( 1992; ). A phylogenetic analysis of the myxobacteria: basis for their classification. . Proc Natl Acad Sci U S A 89:, 9459–9463. [CrossRef] [PubMed]
    [Google Scholar]
  30. Shimkets L. J. , Dworkin M. , Reichenbach H. . ( 2006;). The myxobacteria. . In The Prokaryotes: a Handbook on the Biology of Bacteria, , 3rd edn., vol. 7, pp. 31–115. Edited by Dworkin M. , Falkow S. , Rosenberg E. , Schleifer K.-H. , Stackebrandt E. . , New York:: Springer;.
    [Google Scholar]
  31. Spröer C. , Reichenbach H. , Stackebrandt E. . ( 1999; ). The correlation between morphological and phylogenetic classification of myxobacteria. . Int J Syst Bacteriol 49:, 1255–1262. [CrossRef] [PubMed]
    [Google Scholar]
  32. Stackebrandt E. , Liesack W. . ( 1993; ). Nucleic acids and classification. . In Handbook of New Bacterial Systematics, pp. 152–189. Edited by Goodfellow M. , O’Donnell A. G. . . London:: Academic Press;.
    [Google Scholar]
  33. Turner S. , Pryer K. M. , Miao V. P. W. , Palmer J. D. . ( 1999; ). Investigating deep phylogenetic relationships among cyanobacteria and plastids by small subunit rRNA sequence analysis. . J Eukaryot Microbiol 46:, 327–338. [CrossRef] [PubMed]
    [Google Scholar]
  34. Weissman K. J. , Müller R. . ( 2010; ). Myxobacterial secondary metabolites: bioactivities and modes-of-action. . Nat Prod Rep 27:, 1276–1295. [CrossRef] [PubMed]
    [Google Scholar]
  35. Wenzel S. C. , Müller R. . ( 2009; ). The biosynthetic potential of myxobacteria and their impact in drug discovery. . Curr Opin Drug Discov Devel 12:, 220–230.[PubMed]
    [Google Scholar]
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