1887

Abstract

A Gram-positive, acid-fast and rapidly growing rod, designated F4, was isolated from a soil sample of Haikou in China. The isolate shared 98.2 % 16S rRNA gene sequence similarity with B9-21-178, 96.2 % sequence similarity with FI-05352 and 79.6 % 16S–23S rRNA internal transcribed spacer sequence similarity with B9-21-178. DNA–DNA relatedness between the isolate and DSM 44395 was 43.5 %. The morphological analysis and physiological tests also showed that the isolate differed from any strain reported to date. The mycolic acid profile and the cellular fatty acid composition were also determined. On the basis of phenotypic and chemotaxonomic characteristics and phylogenetic data, it was concluded that strain F4 ( = CGMCC 4.5724 = JCM 17423) merited classification as the type strain of a novel species, for which the name sp. nov. is proposed.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.033449-0
2012-05-01
2020-01-17
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/5/1204.html?itemId=/content/journal/ijsem/10.1099/ijs.0.033449-0&mimeType=html&fmt=ahah

References

  1. Berd D. . ( 1973; ). Laboratory identification of clinically important aerobic actinomycetes. . Appl Microbiol 25:, 665–681.[PubMed]
    [Google Scholar]
  2. Butler W. R. , Floyd M. M. , Silcox V. , Cage G. , Desmond E. , Duffey P. S. , Guthertz L. S. , Gross W. M. , Jost K. C. . & other authors ( 1996; ). Standardized Method for HPLC Identification of Mycobacteria. Atlanta, GA:US Department of Health and Human Services, Public Health Service; Centers for Disease Control and Prevention.
    [Google Scholar]
  3. De Ley J. , Cattoir H. , Reynaerts A. . ( 1970; ). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12:, 133–142. [CrossRef] [PubMed]
    [Google Scholar]
  4. Felsenstein J. . ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  5. Goodfellow M. , Orchard V. A. . ( 1974; ). Antibiotic sensitivity of some nocardioform bacteria and its value as a criterion for taxonomy. . J Gen Microbiol 83:, 375–387.[PubMed] [CrossRef]
    [Google Scholar]
  6. Huß V. A. R. , Festl H. , Schleifer K. H. . ( 1983; ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4:, 184–192. [CrossRef]
    [Google Scholar]
  7. Jahnke K. D. . ( 1992; ). basic computer program for evaluation of spectroscopic DNA renaturation data from Gilford System 2600 spectrophotometer on a PC/XT/AT type personal computer. . J Microbiol Methods 15:, 61–73. [CrossRef]
    [Google Scholar]
  8. Kämpfer P. , Kroppenstedt R. M. . ( 1996; ). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. . Can J Microbiol 42:, 989–1005. [CrossRef]
    [Google Scholar]
  9. Kim H. , Kim S.-H. , Shim T.-S. , Kim M.-n. , Bai G.-H. , Park Y.-G. , Lee S.-H. , Chae G.-T. , Cha C.-Y. . & other authors ( 2005; ). Differentiation of Mycobacterium species by analysis of the heat-shock protein 65 gene (hsp65). . Int J Syst Evol Microbiol 55:, 1649–1656. [CrossRef] [PubMed]
    [Google Scholar]
  10. Kimura M. . ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef] [PubMed]
    [Google Scholar]
  11. Larkin M. A. , Blackshields G. , Brown N. P. , Chenna R. , McGettigan P. A. , McWilliam H. , Valentin F. , Wallace I. M. , Wilm A. . & other authors ( 2007; ). clustal w and clustal_x version 2.0. . Bioinformatics 23:, 2947–2948. [CrossRef] [PubMed]
    [Google Scholar]
  12. Rainey F. A. , Ward-Rainey N. , Kroppenstedt R. M. , Stackebrandt E. . ( 1996; ). The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov.. Int J Syst Bacteriol 46:, 1088–1092. [CrossRef] [PubMed]
    [Google Scholar]
  13. Reischl U. , Melzl H. , Kroppenstedt R. M. , Miethke T. , Naumann L. , Mariottini A. , Mazzarelli G. , Tortoli E. . ( 2006; ). Mycobacterium monacense sp. nov.. Int J Syst Evol Microbiol 56:, 2575–2578. [CrossRef] [PubMed]
    [Google Scholar]
  14. Ringuet H. , Akoua-Koffi C. , Honore S. , Varnerot A. , Vincent V. , Berche P. , Gaillard J. L. , Pierre-Audigier C. . ( 1999; ). hsp65 sequencing for identification of rapidly growing mycobacteria. . J Clin Microbiol 37:, 852–857.[PubMed]
    [Google Scholar]
  15. Rogall T. , Wolters J. , Flohr T. , Böttger E. C. . ( 1990; ). Towards a phylogeny and definition of species at the molecular level within the genus Mycobacterium . . Int J Syst Bacteriol 40:, 323–330. [CrossRef] [PubMed]
    [Google Scholar]
  16. Roth A. , Fischer M. , Hamid M. E. , Michalke S. , Ludwig W. , Mauch H. . ( 1998; ). Differentiation of phylogenetically related slowly growing mycobacteria based on 16S–23S rRNA gene internal transcribed spacer sequences. . J Clin Microbiol 36:, 139–147.[PubMed]
    [Google Scholar]
  17. Roth A. , Reischl U. , Streubel A. , Naumann L. , Kroppenstedt R. M. , Habicht M. , Fischer M. , Mauch H. . ( 2000; ). Novel diagnostic algorithm for identification of mycobacteria using genus-specific amplification of the 16S–23S rRNA gene spacer and restriction endonucleases. . J Clin Microbiol 38:, 1094–1104.[PubMed]
    [Google Scholar]
  18. Sasser M. . ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc.;
  19. Schröder K. H. , Naumann L. , Kroppenstedt R. M. , Reischl U. . ( 1997; ). Mycobacterium hassiacum sp. nov., a new rapidly growing thermophilic mycobacterium. . Int J Syst Bacteriol 47:, 86–91. [CrossRef] [PubMed]
    [Google Scholar]
  20. Shirling E. B. , Gottlieb D. . ( 1966; ). Methods for characterization of Streptomyces species. . Int J Syst Bacteriol 16:, 313–340. [CrossRef]
    [Google Scholar]
  21. Skerman V. B. D. , McGowan V. , Sneath P. H. A. . ( 1980; ). Approved lists of bacterial names. . Int J Syst Bacteriol 30:, 225–420. [CrossRef]
    [Google Scholar]
  22. Smibert R. M. , Krieg N. R. . ( 1994; ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P. , Murray R. G. E. , Wood W. A. , Krieg N. R. . . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  23. Springer B. , Stockman L. , Teschner K. , Roberts G. D. , Böttger E. C. . ( 1996; ). Two-laboratory collaborative study on identification of mycobacteria: molecular versus phenotypic methods. . J Clin Microbiol 34:, 296–303.[PubMed]
    [Google Scholar]
  24. Stackebrandt E. , Goebel B. M. . ( 1994; ). Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44:, 846–849. [CrossRef]
    [Google Scholar]
  25. Stackebrandt E. , Frederiksen W. , Garrity G. M. , Grimont P. A. D. , Kämpfer P. , Maiden M. C. J. , Nesme X. , Rosselló-Mora R. , Swings J. . & other authors ( 2002; ). Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. . Int J Syst Evol Microbiol 52:, 1043–1047. [CrossRef] [PubMed]
    [Google Scholar]
  26. Stanier R. Y. , Palleroni N. J. , Doudoroff M. . ( 1966; ). The aerobic pseudomonads: a taxonomic study. . J Gen Microbiol 43:, 159–271.[PubMed] [CrossRef]
    [Google Scholar]
  27. Tamura K. , Dudley J. , Nei M. , Kumar S. . ( 2007; ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef] [PubMed]
    [Google Scholar]
  28. Tortoli E. , Kroppenstedt R. M. , Bartoloni A. , Caroli G. , Jan I. , Pawlowski J. , Emler S. . ( 1999; ). Mycobacterium tusciae sp. nov.. Int J Syst Bacteriol 49:, 1839–1844. [CrossRef] [PubMed]
    [Google Scholar]
  29. Tortoli E. , Piersimoni C. , Kroppenstedt R. M. , Montoya-Burgos J. I. , Reischl U. , Giacometti A. , Emler S. . ( 2001; ). Mycobacterium doricum sp. nov.. Int J Syst Evol Microbiol 51:, 2007–2012. [CrossRef] [PubMed]
    [Google Scholar]
  30. Wayne L. G. , Brenner D. J. , Colwell R. R. , Grimont P. A. D. , Kandler O. , Krichevsky M. I. , Moore L. H. , Moore W. E. C. , Murray R. G. E. . & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.033449-0
Loading
/content/journal/ijsem/10.1099/ijs.0.033449-0
Loading

Data & Media loading...

Supplements

Supplementary material 

PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error