1887

Abstract

A Gram-stain-positive, strictly aerobic, spherical, non-motile red-pigmented bacterial strain, designated MJ27, was isolated from a sludge sample of the Daejeon sewage disposal plant in South Korea. A polyphasic approach was used to study the taxonomic position of strain MJ27. Strain MJ27 shared highest 16S rRNA gene sequence similarity with DSM 3963 (98.8 %), Ho-08 (97.5 %) and PB314 (96.6 %.); levels of sequence similarity with the type strains of other species were less than 96.0 %. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain MJ27 belonged to the clade formed by members of the genus in the family . The G+C content of the genomic DNA of strain MJ27 was 67.6 mol%. The chemotaxonomic characteristics of strain MJ27 were typical of members of the genus , with MK-8 as the predominant respiratory quinone, Cω7, Cω6, C and C as major fatty acids (>12 %), ornithine as the diamino acid in the cell-wall peptidoglycan and resistance to gamma radiation [D (dose required to reduce the bacterial population by tenfold) >9 kGy]. The low levels of DNA–DNA relatedness reported here (5.3±1.5–29.2±2.3 %) indicate that strain MJ27 represents a species that is separate from its closest relatives in the genus On the basis of phylogenetic inference, fatty acid profile and other phenotypic properties, strain MJ27 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is MJ27 ( = KCTC 13751 = JCM 16918).

Funding
This study was supported by the:
  • , Ministry of Environment of the Republic of Korea , (Award 071-081-133)
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2012-06-01
2020-11-30
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