1887

Abstract

A strictly aerobic, Gram-negative, yellow-pigmented, non-spore-forming rod, designated 15C3, was isolated from aerobic leaf-and-branch compost at EXPO Park in Osaka, Japan. Growth was observed at 9–33 °C (optimum 25 °C) and pH 5.6–7.9 (optimum pH 6.1–7.0). No growth occurred with >2 % (w/v) NaCl. Strain 15C3 reduced nitrate to nitrogen and showed catalase activity but not oxidase activity. The predominant fatty acids were iso-C, iso-C 3-OH and summed feature 3 (comprising Cω7 and/or iso-C 2-OH). The isolate contained phosphatidylethanolamine as the major polar lipid and menaquinone-6 as the major respiratory quinone. The G+C content of the genomic DNA of strain 15C3 was 33.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 15C3 belonged to the genus and was most closely related to WB 4.2-33 (96.9 % sequence similarity). On the basis of phenotypic and phylogenetic distinctiveness, strain 15C3 is considered to represent a novel species in the genus , for which the name sp. nov. is proposed. The type strain is 15C3 ( = KACC 14224  = JCM 16527). Emended descriptions of , and are also given.

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2012-08-01
2021-10-27
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