1887

Abstract

is a well-studied legume symbiont characterized by high genetic stability of the symbiotic plasmid and tolerance to tropical environmental stresses such as high temperature and low soil pH. However, high phenetic and genetic variabilities among strains have been largely reported, with two subgroups, designated type A and B, already defined within the species. A polyphasic study comprising multilocus sequence analysis, phenotypic and genotypic characterizations, including DNA–DNA hybridization, strongly supported the reclassification of type A strains as a novel species. Type A strains formed a well-differentiated clade that grouped with , , , and in the phylogenies of the 16S rRNA, , , , and genes. Several phenotypic traits differentiated type A strains from all related taxa. The novel species, for which the name sp. nov. is proposed, is a broad host range rhizobium being able to establish effective root-nodule symbioses with , , common beans () and . Strain CFN 299 ( = USDA 9039 = LMG 9517 = CECT 4844 = JCM 21088 = IAM 14230 = SEMIA 4083 = CENA 183 = UMR1026 = CNPSo 141) is designated the type strain of sp. nov.

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2012-05-01
2020-01-22
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