1887

Abstract

Several Gram-negative-staining, facultatively anaerobic bacterial isolates were obtained from seedlings showing symptoms of bacterial blight and dieback in Colombia, Rwanda and South Africa. Partial 16S rRNA gene sequencing, together with partial sequencing, placed the isolates in the genus and indicated that they constituted three novel species. Multilocus sequence analysis (MLSA) based on partial sequences of , , and revealed , and as their closest phylogenetic relatives. DNA–DNA hybridization studies confirmed the classification of the new isolates as three novel species and phenotypic tests allowed them to be differentiated from their closest phylogenetic neighbours. The names sp. nov. [type strain LMG 26273 = BD 943 (deposited with the Plant Pathogenic and Plant Protecting Bacteria Collection, South Africa) = BCC 581 (deposited with the Bacterial Culture Collection, Forestry and Agricultural Institute, South Africa)], sp. nov. (type strain LMG 26275 = BD 944 = BCC 571) and sp. nov. (type strain LMG 26277 = BD 946 = BCC 682) are proposed.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.032615-0
2012-07-01
2019-10-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/7/1457.html?itemId=/content/journal/ijsem/10.1099/ijs.0.032615-0&mimeType=html&fmt=ahah

References

  1. Brady C. L., Cleenwerck I., Venter S. N., Vancanneyt M., Swings J., Coutinho T. A.. ( 2008;). Phylogeny and identification of Pantoea species associated with plants, humans and the natural environment based on multilocus sequence analysis (MLSA). . Syst Appl Microbiol 31:, 447–460. [CrossRef][PubMed]
    [Google Scholar]
  2. Brady C. L., Venter S. N., Cleenwerck I., Engelbeen K., Vancanneyt M., Swings J., Coutinho T. A.. ( 2009;). Pantoea vagans sp. nov., Pantoea eucalypti sp. nov., Pantoea deleyi sp. nov. and Pantoea anthophila sp. nov.. Int J Syst Evol Microbiol 59:, 2339–2345. [CrossRef][PubMed]
    [Google Scholar]
  3. Brady C. L., Venter S. N., Cleenwerck I., Vandemeulebroecke K., De Vos P., Coutinho T. A.. ( 2010a;). Transfer of Pantoea citrea, Pantoea punctata and Pantoea terrea to the genus Tatumella emend. as Tatumella citrea comb. nov., Tatumella punctata comb. nov. and Tatumella terrea comb. nov. and description of Tatumella morbirosei sp. nov.. Int J Syst Evol Microbiol 60:, 484–494. [CrossRef][PubMed]
    [Google Scholar]
  4. Brady C. L., Cleenwerck I., Venter S. N., Engelbeen K., De Vos P., Coutinho T. A.. ( 2010b;). Emended description of the genus Pantoea, description of four species from human clinical samples, Pantoea septica sp. nov., Pantoea eucrina sp. nov., Pantoea brenneri sp. nov. and Pantoea conspicua sp. nov., and transfer of Pectobacterium cypripedii (Hori 1911) Brenner et al. 1973 emend. Hauben et al. 1998 to the genus as Pantoea cypripedii comb. nov.. Int J Syst Evol Microbiol 60:, 2430–2440. [CrossRef][PubMed]
    [Google Scholar]
  5. Cleenwerck I., Vandemeulebroecke K., Janssens D., Swings J.. ( 2002;). Re-examination of the genus Acetobacter, with descriptions of Acetobacter cerevisiae sp. nov. and Acetobacter malorum sp. nov.. Int J Syst Evol Microbiol 52:, 1551–1558. [CrossRef][PubMed]
    [Google Scholar]
  6. Coenye T., Falsen E., Vancanneyt M., Hoste B., Govan J. R. W., Kersters K., Vandamme P.. ( 1999;). Classification of Alcaligenes faecalis-like isolates from the environment and human clinical samples as Ralstonia gilardii sp. nov.. Int J Syst Bacteriol 49:, 405–413. [CrossRef][PubMed]
    [Google Scholar]
  7. Coutinho T. A., Preisig O., Mergaert J., Cnockaert M. C., Riedel K. H., Swings J., Wingfield M. J.. ( 2002;). Bacterial blight and dieback of Eucalyptus species, hybrids, and clones in South Africa. . Plant Dis 86:, 20–25. [CrossRef]
    [Google Scholar]
  8. Coutinho T. A., Venter S. N., Roux J, Zhou X. D., Wingfield M. J.. ( 2011;). Bacterial diseases of Eucalyptus. Proceedings of the ACPP APPS 2011 Congress, Darwin, Australia 27–29 April 2011.
  9. Ezaki T., Hashimoto Y., Yabuuchi E.. ( 1989;). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39:, 224–229. [CrossRef]
    [Google Scholar]
  10. Goris J., Suzuki K.-I., De Vos P., Nakase T., Kersters K.. ( 1998;). Evaluation of a microplate DNA-DNA hybridization method compared with the initial renaturation method. . Can J Microbiol 44:, 1148–1153. [CrossRef]
    [Google Scholar]
  11. Grimont P. A. D., Grimont F.. ( 2005;). Genus: Pantoea. . In Bergey’s Manual of Systematic Bacteriology, Volume Two, The Proteobacteria, Part B, The Gammaproteobacteria, , 2nd edn., pp. 713–720. Edited by Brenner D. J., Krieg N. R., Staley J. T... New York:: Springer;.
    [Google Scholar]
  12. Guindon S., Gascuel O.. ( 2003;). A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. . Syst Biol 52:, 696–704. [CrossRef][PubMed]
    [Google Scholar]
  13. Hall T. A.. ( 1999;). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. . Nucleic Acids Symp Ser 41:, 95–98.
    [Google Scholar]
  14. Mergaert J., Verdonck L., Kersters K.. ( 1993;). Transfer of Erwinia ananas (synonym, Erwinia uredovora) and Erwinia stewartii to the genus Pantoea emend. as Pantoea ananas (Serrano 1928) comb. nov. and Pantoea stewartii (Smith 1898) comb. nov., respectively, and description of Pantoea stewartii subsp. indologenes subsp. nov.. Int J Syst Bacteriol 43:, 162–173. [CrossRef]
    [Google Scholar]
  15. Mergaert J., Hauben L., Cnockaert M. C., Swings J.. ( 1999;). Reclassification of non-pigmented Erwinia herbicola strains from trees as Erwinia billingiae sp. nov.. Int J Syst Bacteriol 49:, 377–383. [CrossRef][PubMed]
    [Google Scholar]
  16. Mesbah M., Premachandran U., Whitman W. B.. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  17. Naum M., Brown E. W., Mason-Gamer R. J.. ( 2008;). Is 16S rDNA a reliable phylogenetic marker to characterize relationships below the family level in the Enterobacteriaceae?. J Mol Evol 66:, 630–642. [CrossRef][PubMed]
    [Google Scholar]
  18. Niemann S., Pühler A., Tichy H.-V., Simon R., Selbitschka W.. ( 1997;). Evaluation of the resolving power of three different DNA fingerprinting methods to discriminate among isolates of a natural Rhizobium meliloti population. . J Appl Microbiol 82:, 477–484. [CrossRef][PubMed]
    [Google Scholar]
  19. Popp A., Cleenwerck I., Iversen C., De Vos P., Stephan R.. ( 2010;). Pantoea gaviniae sp. nov. and Pantoea calida sp. nov., isolated from infant formula and an infant formula production environment. . Int J Syst Evol Microbiol 60:, 2786–2792. [CrossRef][PubMed]
    [Google Scholar]
  20. Posada D., Crandall K. A.. ( 1998;). modeltest: testing the model of DNA substitution. . Bioinformatics 14:, 817–818. [CrossRef][PubMed]
    [Google Scholar]
  21. Swart L.. ( 2009;). Pantoea and Xanthomonas species associated with bacterial blight and die-back of Eucalyptus. MSc thesis. University of Pretoria, Pretoria, South Africa.
  22. Swofford D. L.. ( 2000;). paup*: Phylogenetic analysis using parsimony (and other methods) Version 4.0. Sunderland, MA: Sinauer Associates.
  23. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). mega5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef][PubMed]
    [Google Scholar]
  24. Wilson K.. ( 1987;). Preparation of genomic DNA from bacteria. . In Current Protocols in Molecular Biology, pp. 2.4.1–2.4.5. Edited by Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K... New York:: Green Publishing & Wiley-Interscience;.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.032615-0
Loading
/content/journal/ijsem/10.1099/ijs.0.032615-0
Loading

Data & Media loading...

Supplements

Supplementary material 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error