1887

Abstract

A re-evaluation of the taxonomic position of five strains, one assigned to (strain 1330, isolated from spiced meat purchased in Slovakia), two previously assigned to genomospecies 1 (strains NCTC 9529 and 731, isolated from water and a leg infection, respectively) and two previously assigned to (strains 96 and 1435, isolated from onion powder and rye flour, respectively) was carried out. The analysis included phenotypic characterization, 16S rRNA gene sequencing and multilocus sequence analysis (MLSA) of seven housekeeping genes (, , , , , , ; 3036 bp). 16S rRNA gene sequence analysis and MLSA showed that strain 1330 formed an independent phylogenetic lineage in the MLSA, with LMG 23823 as the closest neighbour. DNA–DNA reassociation and phenotypic analysis revealed that strain 1330 represented a novel species, for which the name sp. nov. is proposed (type strain 1330 = CECT 7863 = LMG 26250). Strains NCTC 9529, 731, 96 and 1435 clustered together within an independent phylogenetic lineage, with LMG 23827 as the closest neighbour in the MLSA. DNA–DNA reassociation and phenotypic analysis confirmed that these strains represent a novel species, for which the name sp. nov. is proposed (type strain NCTC 9529 = CECT 7864 = LMG 26249).

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2012-06-01
2019-11-14
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References

  1. Baldwin A. , Loughlin M. , Caubilla-Barron J. , Kucerova E. , Manning G. , Dowson C. , Forsythe S. . ( 2009; ). Multilocus sequence typing of Cronobacter sakazakii and Cronobacter malonaticus reveals stable clonal structures with clinical significance which do not correlate with biotypes. . BMC Microbiol 9:, 223. [CrossRef] [PubMed]
    [Google Scholar]
  2. British Society for Antimicrobial Chemotherapy ( 2010; ). BSAC methods for antimicrobial susceptibility testing, version 9.. http://www.bsac.org.uk/Resources/BSAC/Version_9.1_March_2010_final.pdf.
  3. Caubilla-Barron J. , Hurrell E. , Townsend S. , Cheetham P. , Loc-Carrillo C. , Fayet O. , Prère M.-F. , Forsythe S. J. . ( 2007; ). Genotypic and phenotypic analysis of Enterobacter sakazakii strains from an outbreak resulting in fatalities in a neonatal intensive care unit in France. . J Clin Microbiol 45:, 3979–3985. [CrossRef] [PubMed]
    [Google Scholar]
  4. Chun J. , Lee J.-H. , Jung Y. , Kim M. , Kim S. , Kim B. K. , Lim Y.-W. . ( 2007; ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. . Int J Syst Evol Microbiol 57:, 2259–2261. [CrossRef] [PubMed]
    [Google Scholar]
  5. Duaga C. , Breuwer P. . ( 2008; ). Taxonomy and physiology of Enterobacter sakazakii . . In Enterobacter sakazakii, pp. 1–26. Edited by Farber J. M. , Forsythe S. J. . . Washington, DC:: American Society for Microbiology;.[CrossRef]
    [Google Scholar]
  6. Farmer J. J. III , Asbury M. A. , Hickman F. W. , Brenner D. J. . Enterobacteriaceae study group ( 1980; ). Enterobacter sakazakii: a new species of ‘Enterobacteriaceae’ isolated from clinical specimens. . Int J Syst Bacteriol 30:, 569–584. [CrossRef]
    [Google Scholar]
  7. Forsythe S. J. . ( 2005; ). Enterobacter sakazakii and other bacteria in powdered infant milk formula. . Matern Child Nutr 1:, 44–50. [CrossRef] [PubMed]
    [Google Scholar]
  8. Harayama S. , Kasai H. . ( 2006; ). Bacterial phylogeny reconstruction from molecular sequences. . In Molecular Identification, Systematics, and Population Structure of Prokaryotes, pp. 105–140. Edited by Stackebrandt E. . . Berlin, Heidelberg:: Springer-Verlag.; [CrossRef]
    [Google Scholar]
  9. Ibarz Pavón A. B. , Maiden M. C. . ( 2009; ). Multilocus sequence typing. . Methods Mol Biol 551:, 129–140. [CrossRef] [PubMed]
    [Google Scholar]
  10. Iversen C. , Forsythe S. J. . ( 2004; ). Isolation of Enterobacter sakazakii and other Enterobacteriaceae from powdered infant formula milk and related products. . Food Microbiol 21:, 771–777. [CrossRef]
    [Google Scholar]
  11. Iversen C. , Waddington M. , On S. L. W. , Forsythe S. . ( 2004; ). Identification and phylogeny of Enterobacter sakazakii relative to Enterobacter and Citrobacter species. . J Clin Microbiol 42:, 5368–5370. [CrossRef] [PubMed]
    [Google Scholar]
  12. Iversen C. , Lancashire L. , Waddington M. , Forsythe S. , Ball G. . ( 2006a; ). Identification of Enterobacter sakazakii from closely related species: the use of artificial neural networks in the analysis of biochemical and 16S rDNA data. . BMC Microbiol 6:, 28. [CrossRef] [PubMed]
    [Google Scholar]
  13. Iversen C. , Waddington M. , Farmer J. J. III , Forsythe S. J. . ( 2006b; ). The biochemical differentiation of Enterobacter sakazakii genotypes. . BMC Microbiol 6:, 94. [CrossRef] [PubMed]
    [Google Scholar]
  14. Iversen C. , Lehner A. , Mullane N. , Bidlas E. , Cleenwerck I. , Marugg J. , Fanning S. , Stephan R. , Joosten H. . ( 2007; ). The taxonomy of Enterobacter sakazakii: proposal of a new genus Cronobacter gen. nov. and descriptions of Cronobacter sakazakii comb. nov. Cronobacter sakazakii subsp. sakazakii, comb. nov., Cronobacter sakazakii subsp. malonaticus subsp. nov., Cronobacter turicensis sp. nov., Cronobacter muytjensii sp. nov., Cronobacter dublinensis sp. nov. and Cronobacter genomospecies 1. . BMC Evol Biol 7:, 64. [CrossRef] [PubMed]
    [Google Scholar]
  15. Iversen C. , Mullane N. , McCardell B. , Tall B. D. , Lehner A. , Fanning S. , Stephan R. , Joosten H. . ( 2008; ). Cronobacter gen. nov., a new genus to accommodate the biogroups of Enterobacter sakazakii, and proposal of Cronobacter sakazakii gen. nov., comb. nov., Cronobacter malonaticus sp. nov., Cronobacter turicensis sp. nov., Cronobacter muytjensii sp. nov., Cronobacter dublinensis sp. nov., Cronobacter genomospecies 1, and of three subspecies, Cronobacter dublinensis subsp. dublinensis subsp. nov., Cronobacter dublinensis subsp. lausannensis subsp. nov. and Cronobacter dublinensis subsp. lactaridi subsp. nov.. Int J Syst Evol Microbiol 58:, 1442–1447. [CrossRef] [PubMed]
    [Google Scholar]
  16. Jolley K. A. , Chan M.-S. , Maiden M. C. J. . ( 2004; ). mlstdbNet – distributed multi-locus sequence typing (MLST) databases. . BMC Bioinformatics 5:, 86. [CrossRef] [PubMed]
    [Google Scholar]
  17. Joseph S. , Forsythe S. J. . ( 2011; ). Predominance of Cronobacter sakazakii sequence type 4 in neonatal infections. . Emerg Infect Dis 17:, 1713–1715. [CrossRef] [PubMed]
    [Google Scholar]
  18. Kimura M. . ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef] [PubMed]
    [Google Scholar]
  19. Kucerova E. , Clifton S. W. , Xia X.-Q. , Long F. , Porwollik S. , Fulton L. , Fronick C. , Minx P. , Kyung K. . & other authors ( 2010; ). Genome sequence of Cronobacter sakazakii BAA-894 and comparative genomic hybridization analysis with other Cronobacter species. . PLoS One 5:, e9556. [CrossRef] [PubMed]
    [Google Scholar]
  20. Kucerova E. , Joseph S. , Forsythe S. . ( 2011; ). The Cronobacter genus: ubiquity and diversity. . Quality Assurance and Safety of Crops and Foods 3:, 104–122.[CrossRef]
    [Google Scholar]
  21. Lacher D. W. , Steinsland H. , Blank T. E. , Donnenberg M. S. , Whittam T. S. . ( 2007; ). Molecular evolution of typical enteropathogenic Escherichia coli: clonal analysis by multilocus sequence typing and virulence gene allelic profiling. . J Bacteriol 189:, 342–350. [CrossRef] [PubMed]
    [Google Scholar]
  22. Larkin M. A. , Blackshields G. , Brown N. P. , Chenna R. , McGettigan P. A. , McWilliam H. , Valentin F. , Wallace I. M. , Wilm A. . & other authors ( 2007; ). clustal w and clustal x version 2.0. . Bioinformatics 23:, 2947–2948. [CrossRef] [PubMed]
    [Google Scholar]
  23. Marmur J. . ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. . J Mol Biol 3:, 208–218. [CrossRef]
    [Google Scholar]
  24. Osaili T. , Forsythe S. . ( 2009; ). Desiccation resistance and persistence of Cronobacter species in infant formula. . Int J Food Microbiol 136:, 214–220. [CrossRef] [PubMed]
    [Google Scholar]
  25. Rosselló-Mora R. . ( 2006; ). DNA–DNA reassociation methods applied to microbial taxonomy and their critical evaluation. . In Molecular Identification, Systematics, and Population Structure of Prokaryotes, pp. 23–50. Edited by Stackebrandt E. . . Berlin:: Springer;. [CrossRef]
    [Google Scholar]
  26. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  27. Stackebrandt E. , Goebel B. M. . ( 1994; ). Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44:, 846–849. [CrossRef]
    [Google Scholar]
  28. Tamura K. , Dudley J. , Nei M. , Kumar S. . ( 2007; ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef] [PubMed]
    [Google Scholar]
  29. Townsend S. M. , Hurrell E. , Caubilla-Barron J. , Loc-Carrillo C. , Forsythe S. J. . ( 2008; ). Characterization of an extended spectrum beta-lactamase Enterobacter hormaechei nosocomial outbreak, and other Enterobacter hormaechei misidentified as Cronobacter (Enterobacter)sakazakii . . Microbiology 154:, 3659–3667. [CrossRef] [PubMed]
    [Google Scholar]
  30. Turcovský I. , Kuniková K. , Drahovská H. , Kaclíková E. . ( 2011; ). Biochemical and molecular characterization of Cronobacter spp. (formerly Enterobacter sakazakii) isolated from foods. . Antonie van Leeuwenhoek 99:, 257–269. [CrossRef] [PubMed]
    [Google Scholar]
  31. Urdiain M. , López-López A. , Gonzalo C. , Busse H. J. , Langer S. , Kämpfer P. , Rosselló-Móra R. . ( 2008; ). Reclassification of Rhodobium marinum and Rhodobium pfennigii as Afifella marina gen. nov. comb. nov. and Afifella pfennigii comb. nov., a new genus of photoheterotrophic Alphaproteobacteria and emended descriptions of Rhodobium, Rhodobium orientis and Rhodobium gokarnense . . Syst Appl Microbiol 31:, 339–351. [CrossRef] [PubMed]
    [Google Scholar]
  32. Wayne L. G. , Brenner D. J. , Colwell R. R. , Grimont P. A. D. , Kandler O. , Krichevsky M. I. , Moore L. H. , Moore W. E. C. , Murray R. G. E. . & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
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