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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 62, Issue Pt_5

sp. nov., a phototrophic alphaproteobacterium isolated from aquatic sediments Free

Abstract

Two strains (JA492 and JA590) of spiral-shaped, anaerobic, Gram-stain-negative, motile, purple non-sulfur bacteria were isolated from aquatic sediments from a bird sanctuary and a stream, respectively, and were characterized by a polyphasic taxonomic approach. Bacteriochlorophyll and carotenoids (rhodopin, lycopene, hydroxylycopene glucoside and dihydroxylycopene diglucoside) were present as photosynthetic pigments. Intracellular photosynthetic membranes were of the stacked type. The major fatty acids were Cω7, C and summed feature 3 (Cω6 and/or Cω7) in both strains. Ubiquinones and menaquinones were present as major quinone components. The genomic DNA G+C contents of strains JA492 and JA590 were 63.8 and 61.5 mol%, respectively. Both strains were closely related (mean DNA–DNA hybridization >70 %). Phylogenetic analysis showed that the strains clustered with species of the genus of the family , class . Based on 16S rRNA gene sequence analysis, both strains showed highest sequence similarity with JA317 (97.2–97.4 %), DSM 120 (96.5–96.7 %), DSM 113 (96.7–96.9 %) and JA145 (96.5–96.7 %). DNA–DNA relatedness between strain JA492 and its closest relative in the genus was less than 42 %. It is evident from phenotypic, chemotaxonomic and molecular genetic data that strain JA492 represents a novel species of the genus , for which the name sp. nov. is proposed; the type strain is JA492 ( = NBRC 107650 = KCTC 15012).

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Funding
This study was supported by the:
  • Department of Biotechnology, Government of India
© 2012 IUMS
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2012-05-01
2024-12-12
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References

  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. 1990; Basic local alignment search tool. J Mol Biol 215:403–410[PubMed] [CrossRef]
     [Google Scholar]
  2. Anil Kumar P., Srinivas T. N. R., Takaichi S., Maoka T., Sasikala Ch., Ramana Ch. V. 2009; Phaeospirillum chandramohanii sp. nov., a phototrophic alphaproteobacterium with carotenoid glycosides. Int J Syst Evol Microbiol 59:2089–2093 [View Article][PubMed]
     [Google Scholar]
  3. Brosius J., Palmer M. L., Kennedy P. J., Noller H. F. 1978; Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli . Proc Natl Acad Sci U S A 75:4801–4805 [View Article][PubMed]
     [Google Scholar]
  4. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [View Article][PubMed]
     [Google Scholar]
  5. Guindon S., Lethiec F., Duroux P., Gascuel O. 2005; PHYML Online – a web server for fast maximum likelihood-based phylogenetic inference. Nucleic Acids Res 33:Web ServerW557–W559 [View Article]
     [Google Scholar]
  6. Hall T. A. 1999; BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98
     [Google Scholar]
  7. Hanada S., Takaichi S., Matsuura K., Nakamura K. 2002; Roseiflexus castenholzii gen. nov., sp. nov., a thermophilic, filamentous, photosynthetic bacterium that lacks chlorosomes. Int J Syst Evol Microbiol 52:187–193[PubMed] [CrossRef]
     [Google Scholar]
  8. Hiraishi A., Hoshino Y. 1984; Distribution of rhodoquinone in Rhodospirillaceae and its taxonomic implications. J Gen Appl Microbiol 30:435–448 [View Article]
     [Google Scholar]
  9. Imhoff J. F. 1984; Quinones of phototrophic purple bacteria. FEMS Microbiol Lett 25:85–89 [View Article]
     [Google Scholar]
  10. Imhoff J. F. 2005a; Genus I. Rhodospirillum Molisch 1907, 24AL emend. Imhoff, Petri and Süling 1998, 796. In Bergey’s Manual of Systematic Bacteriology, 2nd edn. vol. 2C pp. 1–6 Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M. New York: Springer; [View Article]
     [Google Scholar]
  11. Imhoff J. F. 2005b; Genus V. Phaeospirillum Imhoff, Petri and Süling 1998, 796VP . In Bergey’s Manual of Systematic Bacteriology, 2nd edn. vol. 2C pp. 32–33 Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M. New York: Springer; [View Article]
     [Google Scholar]
  12. Imhoff J. F., Pfennig N. 2001; Thioflavicoccus mobilis gen. nov., sp. nov., a novel purple sulfur bacterium with bacteriochlorophyll b . Int J Syst Evol Microbiol 51:105–110[PubMed]
     [Google Scholar]
  13. Imhoff J. F., Petri R., Süling J. 1998a; Reclassification of species of the spiral-shaped phototrophic purple non-sulfur bacteria of the alpha-Proteobacteria: description of the new genera Phaeospirillum gen. nov., Rhodovibrio gen. nov., Rhodothalassium gen. nov. and Roseospira gen. nov. as well as transfer of Rhodospirillum fulvum to Phaeospirillum fulvum comb. nov., of Rhodospirillum molischianum to Phaeospirillum molischianum comb. nov., of Rhodospirillum salinarum to Rhodovibrio salinarum comb. nov., of Rhodospirillum sodomense to Rhodovibrio sodomensis comb. nov., of Rhodospirillum salexigens to Rhodothalassium salexigens comb. nov. and of Rhodospirillum mediosalinum to Roseospira mediosalina comb. nov.. Int J Syst Bacteriol 48:793–798 [View Article][PubMed]
     [Google Scholar]
  14. Imhoff J. F., Süling J., Petri R. 1998b; Phylogenetic relationships among the Chromatiaceae, their taxonomic reclassification and description of the new genera Allochromatium, Halochromatium, Isochromatium, Marichromatium, Thiococcus, Thiohalocapsa and Thermochromatium . Int J Syst Bacteriol 48:1129–1143 [View Article][PubMed]
     [Google Scholar]
  15. Lakshmi K. V. N. S., Sasikala Ch., Takaichi S., Ramana Ch. V. 2011a; Phaeospirillum oryzae sp. nov., a spheroplast-forming, phototrophic alphaproteobacterium from a paddy soil. Int J Syst Evol Microbiol 61:1656–1661 [View Article][PubMed]
     [Google Scholar]
  16. Lakshmi K. V. N. S., Sasikala Ch., Ashok Kumar G. V., Chandrasekaran R., Ramana Ch. V. 2011b; Phaeovibrio sulfidiphilus gen. nov., sp. nov., phototrophic alphaproteobacteria isolated from brackish water. Int J Syst Evol Microbiol 61:828–833 [View Article][PubMed]
     [Google Scholar]
  17. Lanave C., Preparata G., Saccone C., Serio G. 1984; A new method for calculating evolutionary substitution rates. J Mol Evol 20:86–93 [View Article][PubMed]
     [Google Scholar]
  18. Lane D. J., Pace B., Olsen G. J., Stahl D. A., Sogin M. L., Pace N. R. 1985; Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses. Proc Natl Acad Sci U S A 82:6955–6959 [View Article][PubMed]
     [Google Scholar]
  19. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [View Article]
     [Google Scholar]
  20. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
     [Google Scholar]
  21. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A 74:5463–5467 [View Article][PubMed]
     [Google Scholar]
  22. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.;
  23. Takaichi S., Shimada K. 1992; Characterization of carotenoids in photosynthetic bacteria. Methods Enzymol 213:374–385 [View Article]
     [Google Scholar]
  24. Takaichi S., Maoka T., Sasikala Ch., Ramana Ch. V., Shimada K. 2011; Genus specific unusual carotenoids in purple bacteria, Phaeospirillum and Roseospira: structures and biosyntheses. Curr Microbiol 63:75–80 [View Article][PubMed]
     [Google Scholar]
  25. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [View Article][PubMed]
     [Google Scholar]
  26. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
     [Google Scholar]
  27. Tourova T. P., Antonov A. S. 1988; Identification of microorganisms by rapid DNA-DNA hybridization. Meth Microbiol 19:333–355 [View Article]
     [Google Scholar]
  28. Trüper H. G., Pfennig N. 1981; Isolation of members of the families Chromatiaceae and Chlorobiaceae . In The Prokaryotes: a Handbook on Habitats, Isolation, and Identification of Bacteria pp. 279–289 Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. G. Berlin: Springer;
     [Google Scholar]
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Phaeospirillum tilakii sp. nov., a phototrophic alphaproteobacterium isolated from aquatic sediments
Int J Syst Evol Microbiol 62, 1069 (2012); https://doi.org/10.1099/ijs.0.032250-0
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