1887

Abstract

Two strains (JA492 and JA590) of spiral-shaped, anaerobic, Gram-stain-negative, motile, purple non-sulfur bacteria were isolated from aquatic sediments from a bird sanctuary and a stream, respectively, and were characterized by a polyphasic taxonomic approach. Bacteriochlorophyll and carotenoids (rhodopin, lycopene, hydroxylycopene glucoside and dihydroxylycopene diglucoside) were present as photosynthetic pigments. Intracellular photosynthetic membranes were of the stacked type. The major fatty acids were Cω7, C and summed feature 3 (Cω6 and/or Cω7) in both strains. Ubiquinones and menaquinones were present as major quinone components. The genomic DNA G+C contents of strains JA492 and JA590 were 63.8 and 61.5 mol%, respectively. Both strains were closely related (mean DNA–DNA hybridization >70 %). Phylogenetic analysis showed that the strains clustered with species of the genus of the family , class . Based on 16S rRNA gene sequence analysis, both strains showed highest sequence similarity with JA317 (97.2–97.4 %), DSM 120 (96.5–96.7 %), DSM 113 (96.7–96.9 %) and JA145 (96.5–96.7 %). DNA–DNA relatedness between strain JA492 and its closest relative in the genus was less than 42 %. It is evident from phenotypic, chemotaxonomic and molecular genetic data that strain JA492 represents a novel species of the genus , for which the name sp. nov. is proposed; the type strain is JA492 ( = NBRC 107650 = KCTC 15012).

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2012-05-01
2019-12-11
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