1887

Abstract

Bacteria were isolated from black lesions on shoots of European pear trees ( L.) in an orchard in Japan. Previous characterization of this novel pathogen by phenotypic and genotypic methods suggested that it should belong to the genus but might not correspond to either or . Here, phylogenetic analyses of the 16S rRNA gene, , and gene sequences indicated that it could not be assigned to any recognized species of the genus . DNA–DNA hybridization confirmed that the bacterial strains represented a novel species. The DNA G+C contents, the fatty acid profile and phenotypic characteristics resembled those previously reported for members of the genus . On the basis of these and previous results, the pathogen represents a novel species of the genus , for which the name sp. nov. (type strain: YPPS 951 = LMG 25843 = NCPPB 4475) is proposed.

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2012-08-01
2019-12-06
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References

  1. Altschul S. F. , Gish W. , Miller W. , Myers E. W. , Lipman D. J. . ( 1990; ). Basic local alignment search tool. . J Mol Biol 215:, 403–410.[PubMed] [CrossRef]
    [Google Scholar]
  2. Dye D. W. . ( 1968; ). A taxonomic study of the genus Erwinia I. The ‘Amylovora’ group. . N Z J Sci 11:, 590–607.
    [Google Scholar]
  3. Ezaki T. , Hashimoto Y. , Yabuuchi E. . ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39:, 224–229.[CrossRef]
    [Google Scholar]
  4. Felsenstein J. . ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  5. Geider K. , Auling G. , Du Z. , Jakovljevic V. , Jock S. , Völksch B. . ( 2006; ). Erwinia tasmaniensis sp. nov., a non-phytopathogenic bacterium from apple and pear trees. . Int J Syst Evol Microbiol 56:, 2937–2943. [CrossRef] [PubMed]
    [Google Scholar]
  6. Hasegawa M. , Kishino H. , Yano T. . ( 1985; ). Dating of the human-ape splitting by a molecular clock of mitochondrial DNA. . J Mol Evol 22:, 160–174. [CrossRef] [PubMed]
    [Google Scholar]
  7. Hauben L. , Swings J. . ( 2005; ). Genus XIII Erwinia . . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 2B, pp. 670–679. Edited by Brenner D. J. , Krieg N. R. , Staley J. T. , Garrity G. M. . . New York:: Springer;.
    [Google Scholar]
  8. Jobb G. , von Haeseler A. , Strimmer K. . ( 2004; ). TREEFINDER: a powerful graphical analysis environment for molecular phylogenetics. . BMC Evol Biol 4:, 18. [CrossRef] [PubMed]
    [Google Scholar]
  9. Katayama-Fujimura Y. , Komatsu Y. , Kuraishi H. , Kaneko T. . ( 1984; ). Estimation of DNA base composition by high performance liquid chromatography of its nuclease P1 hydrolysate. . Agric Biol Chem 48:, 3169–3172. [CrossRef]
    [Google Scholar]
  10. Kim W.-S. , Gardan L. , Rhim S.-L. , Geider K. . ( 1999; ). Erwinia pyrifoliae sp. nov., a novel pathogen that affects Asian pear trees (Pyrus pyrifolia Nakai). . Int J Syst Bacteriol 49:, 899–906. [CrossRef] [PubMed]
    [Google Scholar]
  11. King E. O. , Ward M. K. , Raney D. E. . ( 1954; ). Two simple media for the demonstration of phycocyanin and fluorescin. . J Lab Clin Med 44:, 301–307.
    [Google Scholar]
  12. Larkin M. A. , Blackshields G. , Brown N. P. , Chenna R. , McGettigan P. A. , McWilliam H. , Valentin F. , Wallace I. M. , Wilm A. . & other authors ( 2007; ). clustal w and clustal_x version 2.0. . Bioinformatics 23:, 2947–2948. [CrossRef] [PubMed]
    [Google Scholar]
  13. López M. M. , Roselló M. , Llop P. , Ferrer S. , Christen R. , Gardan L. . ( 2011; ). Erwinia piriflorinigrans sp. nov., a novel pathogen that causes necrosis of pear blossoms. . Int J Syst Evol Microbiol 61:, 561–567. [CrossRef] [PubMed]
    [Google Scholar]
  14. Matsuura T. , Shinohara H. , Inoue Y. , Azegami K. , Tsushima S. , Tsukamoto T. , Mizuno A. . ( 2007; ). Erwinia isolates from the bacterial shoot blight of pear in Japan are closely related to Erwinia pyrifoliae based on phylogenetic analyses of gyrB and rpoD genes. . J Gen Plant Pathol 73:, 53–58. [CrossRef]
    [Google Scholar]
  15. Mizuno A. , Sato S. , Kawai A. , Nishiyama K. . ( 2000; ). Taxonomic position of the causal pathogen of bacterial shoot blight of pear. . J Gen Plant Pathol 66:, 48–58. [CrossRef]
    [Google Scholar]
  16. Mizuno A. , Tsukamoto T. , Shimizu Y. , Ooya H. , Matsuura T. , Saito N. , Sato S. , Kikuchi S. , Uzuki T. , Azegami K. . ( 2010; ). Occurrence of bacterial black shoot disease of European pear in Yamagata Prefecture. . J Gen Plant Pathol 76:, 43–51. [CrossRef]
    [Google Scholar]
  17. Paisley R. . ( 1996; ). MIS Whole Cell Fatty Acid Analysis by Gas Chromatography Training Manual. Newark, DE:: MIDI, Inc;.
    [Google Scholar]
  18. Rhim S.-L. , Völksch B. , Gardan L. , Paulin J.-P. , Langlotz C. , Kim W.-S. , Geider K. . ( 1999; ). Erwinia pyrifoliae, an Erwinia species different from Erwinia amylovora, causes a necrotic disease of Asian pear trees. . Plant Pathol 48:, 514–520. [CrossRef]
    [Google Scholar]
  19. Roselló M. , Peñalver J. , Llop P. , Gorris M. T. , Cambra M. , López M. M. , Chartier R. , García F. , Montón C. . ( 2006; ). Identification of an Erwinia sp. different from Erwinia amylovora and responsible for necrosis on pear blossoms. . Can J Plant Pathol 28:, 30–41. [CrossRef]
    [Google Scholar]
  20. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  21. Sawada H. , Kunugi Y. , Watauchi K. , Kudo A. , Sato T. . ( 2011; ). [Bacterial spot, a new disease of grapevine (Vitis vinifera) caused by Xanthomonas arboricola]. . Jpn J Phytopathol 77:, 7–22 (in Japanese). [CrossRef]
    [Google Scholar]
  22. Van der Zwet T. , Keil H. L. . ( 1979; ). Fire blight: a bacterial disease of rosaceous plants. . Symptomatology (chapter 4), pp. 21–26. Washington, DC:: US Dept of Agriculture, Science and Education Administration;.
  23. Wakimoto S. . ( 1955; ). [Studies on multiplication of OP1 phage (Xanthomonas oryzae bacteriophage). 1. One-step growth experiment under various condition]. . Sci. Bull Fac Agric Kyushu Univ 15:, 151–160 (in Japanese).
    [Google Scholar]
  24. Yamamoto S. , Harayama S. . ( 1995; ). PCR amplification and direct sequencing of gyrB genes with universal primers and their application to the detection and taxonomic analysis of Pseudomonas putida strains. . Appl Environ Microbiol 61:, 1104–1109.[PubMed]
    [Google Scholar]
  25. Yamamoto S. , Harayama S. . ( 1998; ). Phylogenetic relationships of Pseudomonas putida strains deduced from the nucleotide sequences of gyrB, rpoD and 16S rRNA genes. . Int J Syst Bacteriol 48:, 813–819. [CrossRef] [PubMed]
    [Google Scholar]
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