1887

Abstract

Two Gram-positive, moderately thermophilic, endospore-forming, rod-shaped, motile bacteria, designated PDF25 and PDF30, were isolated from Karakoc hot spring in the province of Izmir, Turkey, and were characterized in order to determine their phylogenetic positions. 16S rRNA gene sequence analysis revealed that the two strains belonged to the genus ; strain PDF25 showed highest sequence similarity to strain PDF30 (99.4 %) and DSM 7064 (98.5 %). The major fatty acids of strain PDF25 were iso-C (39.30 %), anteiso-C (26.10 %) and iso-C (14.75 %). Polar lipid analysis revealed diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, and a variety of unidentified aminophospholipids, phospholipids and aminolipids. The major isoprenoid quinone was MK-7. The G+C content of the genomic DNA was 56.09 mol%. DNA–DNA hybridization experiments revealed 58 % relatedness between strain PDF25 and DSM 7064. Based on these data, the two strains are considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is PDF25 ( = DSM 24395 = LMG 26289).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.031914-0
2012-04-01
2019-12-08
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/4/849.html?itemId=/content/journal/ijsem/10.1099/ijs.0.031914-0&mimeType=html&fmt=ahah

References

  1. Allan R. N. , Lebbe L. , Heyrman J. , De Vos P. , Buchanan C. J. , Logan N. A. . ( 2005; ). Brevibacillus levickii sp. nov. and Aneurinibacillus terranovensis sp. nov., two novel thermoacidophiles isolated from geothermal soils of northern Victoria Land, Antarctica. . Int J Syst Evol Microbiol 55:, 1039–1050. [CrossRef] [PubMed]
    [Google Scholar]
  2. Atlas R. M. . ( 1993; ). Handbook of Microbiological Media. Edited by Parks L. C. . . Boca Raton, FL:: CRC Press;.
    [Google Scholar]
  3. Baek S.-H. , Im W.-T. , Oh H. W. , Lee J.-S. , Oh H.-M. , Lee S.-T. . ( 2006; ). Brevibacillus ginsengisoli sp. nov., a denitrifying bacterium isolated from soil of a ginseng field. . Int J Syst Evol Microbiol 56:, 2665–2669. [CrossRef] [PubMed]
    [Google Scholar]
  4. Beffa T. , Blanc M. , Lyon P. F. , Vogt G. , Marchiani M. , Fischer J. L. , Aragno M. . ( 1996; ). Isolation of Thermus strains from hot composts (60 to 80 °C). . Appl Environ Microbiol 62:, 1723–1727.[PubMed]
    [Google Scholar]
  5. Belduz A. O. , Dulger S. , Demirbag Z. . ( 2003; ). Anoxybacillus gonensis sp. nov., a moderately thermophilic, xylose-utilizing, endospore-forming bacterium. . Int J Syst Evol Microbiol 53:, 1315–1320.[CrossRef]
    [Google Scholar]
  6. Brosius J. , Palmer M. L. , Kennedy P. J. , Noller H. F. . ( 1978; ). Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli . . Proc Natl Acad Sci U S A 75:, 4801–4805. [CrossRef] [PubMed]
    [Google Scholar]
  7. Chun J. , Lee J.-H. , Jung Y. , Kim M. , Kim S. , Kim B. K. , Lim Y.-W. . ( 2007; ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. . Int J Syst Evol Microbiol 57:, 2259–2261. [CrossRef] [PubMed]
    [Google Scholar]
  8. De Ley J. , Cattoir H. , Reynaerts A. . ( 1970; ). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12:, 133–142. [CrossRef] [PubMed]
    [Google Scholar]
  9. Dussault H. P. . ( 1955; ). An improved technique for staining red halophilic bacteria. . J Bacteriol 70:, 484–485.[PubMed]
    [Google Scholar]
  10. Felsenstein J. . ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef] [PubMed]
    [Google Scholar]
  11. Felsenstein J. . ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  12. Fitch W. M. . ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20:, 406–416. [CrossRef]
    [Google Scholar]
  13. Goto K. , Fujita R. , Kato Y. , Asahara M. , Yokota A. . ( 2004; ). Reclassification of Brevibacillus brevis strains NCIMB 13288 and DSM 6472 ( = NRRL NRS-887) as Aneurinibacillus danicus sp. nov. and Brevibacillus limnophilus sp. nov.. Int J Syst Evol Microbiol 54:, 419–427. [CrossRef] [PubMed]
    [Google Scholar]
  14. Hall T. A. . ( 1999; ). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. . Nucleic Acids Symp Ser 41:, 95–98.
    [Google Scholar]
  15. Huß V. A. R. , Festl H. , Schleifer K. H. . ( 1983; ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4:, 184–192. [CrossRef]
    [Google Scholar]
  16. Kimura M. . ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef] [PubMed]
    [Google Scholar]
  17. Kuykendall L. D. , Roy M. A. , O’Neill J. J. , Devine T. E. . ( 1988; ). Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum . . Int J Syst Bacteriol 38:, 358–361. [CrossRef]
    [Google Scholar]
  18. Logan N. A. , Forsyth G. , Lebbe L. , Goris J. , Heyndrickx M. , Balcaen A. , Verhelst A. , Falsen E. , Ljungh A. . & other authors ( 2002; ). Polyphasic identification of Bacillus and Brevibacillus strains from clinical, dairy and industrial specimens and proposal of Brevibacillus invocatus sp. nov.. Int J Syst Evol Microbiol 52:, 953–966. [CrossRef] [PubMed]
    [Google Scholar]
  19. Mandel M. , Marmur J. . ( 1968; ). Use of ultraviolet absorbance-temperature profile for determining the guanine plus cytosine content of DNA. . Methods Enzymol 12B:, 195–206. [CrossRef]
    [Google Scholar]
  20. Marmur J. . ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. . J Mol Biol 3:, 208–218. [CrossRef]
    [Google Scholar]
  21. Migula W. . ( 1900; ). System der Bakterien, vol. 2. Jena:: Gustav Fischer;.
    [Google Scholar]
  22. Miller L. T. . ( 1982; ). Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. . J Clin Microbiol 16:, 584–586.[PubMed]
    [Google Scholar]
  23. Powers E. M. . ( 1995; ). Efficacy of the Ryu nonstaining KOH technique for rapidly determining gram reactions of food-borne and waterborne bacteria and yeasts. . Appl Environ Microbiol 61:, 3756–3758.[PubMed]
    [Google Scholar]
  24. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  25. Shida O. , Takagi H. , Kadowaki K. , Komagata K. . ( 1996; ). Proposal for two new genera, Brevibacillus gen. nov. and Aneurinibacillus gen. nov.. Int J Syst Bacteriol 46:, 939–946. [CrossRef] [PubMed]
    [Google Scholar]
  26. Smibert R. M. , Krieg N. R. . ( 1994; ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P. , Murray R. G. E. , Wood W. A. , Krieg N. R. . . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  27. Stackebrandt E. , Goebel B. M. . ( 1994; ). Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44:, 846–849. [CrossRef]
    [Google Scholar]
  28. Swofford D. L. . ( 2002; ). paup*: Phylogenetic analysis using parsimony (and other methods), version 4. . Sunderland, MA:: Sinauer Associates;.
  29. Tamura K. , Dudley J. , Nei M. , Kumar S. . ( 2007; ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef] [PubMed]
    [Google Scholar]
  30. Thompson J. D. , Gibson T. J. , Plewniak F. , Jeanmougin F. , Higgins D. G. . ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef] [PubMed]
    [Google Scholar]
  31. Tindall B. J. . ( 1990a; ). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. . Syst Appl Microbiol 13:, 128–130. [CrossRef]
    [Google Scholar]
  32. Tindall B. J. . ( 1990b; ). Lipid composition of Halobacterium lacusprofundi . . FEMS Microbiol Lett 66:, 199–202. [CrossRef]
    [Google Scholar]
  33. Tindall B. J. , Sikorski J. , Smibert R. M. , Krieg N. R. . ( 2007; ). Phenotypic characterization and the principles of comparative systematics. . In Methods for General and Molecular Microbiology, , 3rd edn., pp. 330–393. Edited by Reddy C. A. , Beveridge T. J. , Breznak J. A. , Marzluf G. , Schmidt T. M. , Snyder L. R. . . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  34. Wayne L. G. , Brenner D. J. , Colwell R. R. , Grimont P. A. D. , Kandler O. , Krichevsky M. I. , Moore L. H. , Moore W. E. C. , Murray R. G. E. . & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.031914-0
Loading
/content/journal/ijsem/10.1099/ijs.0.031914-0
Loading

Data & Media loading...

Supplements

Supplementary figures 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error