1887

Abstract

Two strains of lactic acid bacteria, designated SU 18 and SU 83, were isolated from silage prepared with Sudan grass [ (Piper) Stapf.]. The isolates were Gram-stain-positive, catalase-negative, facultatively anaerobic rods that did not produce gas from glucose. The isolates exhibited ≥93.5 % DNA–DNA relatedness to each other and shared the same phenotypic characteristics, which indicated that they belonged to a single species. The DNA G+C content was 58.5–59.2 mol%. On the basis of 16S rRNA gene sequence analysis, the isolates were placed in the genus . Their closest phylogenetic neighbours were JCM 12514 and JCM 13995 (95.9 and 96.8 % 16S rRNA gene sequence similarity, respectively, with strain SU 18). Ribotyping revealed that strain SU 18 was well separated from JCM 12514 and JCM 13995. Strain SU 18 exhibited ≤23.7 % DNA–DNA relatedness with its closest phylogenetic neighbours. The isolates represent a novel species in the genus , for which the name sp. nov. is proposed. The type strain is SU 18 ( = JCM 17158  = CGMCC 1.10801). The description of the genus is also amended.

Funding
This study was supported by the:
  • , Integrated Research for Developing Japanese-style Forage Feeding System to Increase Forage Self-Support Ration
  • , Ministry of Agriculture, Forestry and Fisheries of Japan
  • , Introduction of Microbial Genetic Resources in Foreign Countries
  • , National Institute of Agrobiological Science of Japan
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2012-05-01
2020-09-29
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