1887

Abstract

The morphology of three phenetically identical isolates, collected from the bioaerosol in a restoration laboratory in Italy, displayed macro- and microscopic characteristics that were similar though not completely ascribable to . For this reason, a phylogenetic approach based on DNA sequencing analysis was performed to establish both the taxonomic status and the evolutionary relationships of these three peculiar isolates in relation to previously described species of the genus . We used four nuclear loci (both rRNA and protein coding genes) that have previously proved useful for the molecular investigation of taxa belonging to the genus at various evolutionary levels. The internal transcribed spacer region (ITS1–5.8S–ITS2), domains D1 and D2 of the 28S rDNA, a region of the tubulin beta chain gene () and part of the calmodulin gene () were amplified by PCR and sequenced. Analysis of the rRNA genes and of the and sequence data indicates the presence of three isogenic isolates belonging to a genetically distinct species of the genus , here described and named sp. nov. (ATCC MYA-4591  = CBS 129191). This novel species is phylogenetically different from and other related species of the genus (e.g. ), from which it can be distinguished on the basis of morphological trait analysis.

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2012-02-01
2019-10-20
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