1887

Abstract

Strain AM-8B5, isolated from Lake Sevan in Armenia, was characterized phenotypically, chemotaxonomically and phylogenetically. This chemo-organoheterotrophic, aerobic, facultatively anaerobic, catalase- and oxidase-positive, non-motile strain grew on NSY medium at NaCl concentrations of 0.0–0.2 % (w/v) and at 4–30 °C. Whole-cell fatty acids were dominated by summed feature 3 (including Cω7 and iso-C 2-OH), C and Cω7. C 2-OH and C 2-OH were the only hydroxylated fatty acids detected. Phylogenetic analysis as well as phenotypic and chemotaxonomic similarities indicated that the novel isolate was affiliated with the genus . 16S rRNA gene similarity values with the four previously described species ranged from 96.2 to 98.7 %. DNA–DNA hybridization experiments showed that the isolate did not belong to any of the previously described species. The isolate could be distinguished from all previously established species based on chemotaxonomic and phenotypic traits. The bacterium possessed a free-living lifestyle and represents a group of bacteria inhabiting the water column of many freshwater lakes. Based on the revealed phylogeny, and chemotaxonomic and phenotypic differences to previously described species, it is proposed that the isolate represents a novel species, sp. nov.; the type strain is AM-8B5 ( = DSM 22349 = CIP 110078).

Funding
This study was supported by the:
  • Austrian Exchange Service (OeAD)
  • Austrian Science Fund (Award P19853)
  • ESF project FREDI (Award FWF I 482-B09)
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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2012-02-01
2022-01-29
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