1887

Abstract

A Gram-positive, non-motile, rod-shaped, psychrophilic actinomycete, designated strain Cr7-14, was isolated from alpine glacier cryoconite. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain Cr7-14 was related to members of the genus and shared highest 16S rRNA gene sequence similarities with the type strains of (98.6 %), (98.2 %), (98.1 %) and (97.6 %). The predominant cellular fatty acids of strain Cr7-14 were Cω8 (39.5 %) and iso-C (32.4 %). The major menaquinone was MK-8(H). The diagnostic diamino acid in the cell-wall peptidoglycan was -2,6-diaminopimelic acid. The predominant cell-wall sugars were galactose and rhamnose. The polar lipid pattern contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylinositol, four unknown glycolipids and two unknown polar lipids. The genomic DNA G+C content was 71.9 mol%. On the basis of phenotypic characteristics, phylogenetic analysis and DNA–DNA relatedness data, a novel species, sp. nov., is proposed, with Cr7-14 ( = DSM 23325 = LMG 26053 = CGMCC 1.10697) as the type strain.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.031047-0
2012-02-01
2019-10-23
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/2/445.html?itemId=/content/journal/ijsem/10.1099/ijs.0.031047-0&mimeType=html&fmt=ahah

References

  1. Cavicchioli R. . ( 2006; ). Cold-adapted archaea. . Nat Rev Microbiol 4:, 331–343. [CrossRef] [PubMed]
    [Google Scholar]
  2. Cho C. H. , Lee J.-S. , An D.-S. , Whon T. W. , Kim S.-G. . ( 2010; ). Nocardioides panacisoli sp. nov., isolated from the soil of a ginseng field. . Int J Syst Evol Microbiol 60:, 387–392. [CrossRef] [PubMed]
    [Google Scholar]
  3. Choi D. H. , Kim H. M. , Noh J. H. , Cho B. C. . ( 2007; ). Nocardioides marinus sp. nov.. Int J Syst Evol Microbiol 57:, 775–779. [CrossRef] [PubMed]
    [Google Scholar]
  4. Collins M. D. . ( 1985; ). Isoprenoid quinone analysis in classification and identification. . In Chemical Methods in Bacterial Systematics, pp. 267–287. Edited by Goodfellow M. , Minnikin D. E. . . London:: Academic Press;.
    [Google Scholar]
  5. De Ley J. , Cattoir H. , Reynaerts A. . ( 1970; ). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12:, 133–142. [CrossRef] [PubMed]
    [Google Scholar]
  6. Felsenstein J. . ( 2009; ). phylip (phylogeny inference package), version 3.69. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  7. Huß V. A. R. , Festl H. , Schleifer K. H. . ( 1983; ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4:, 184–192.[CrossRef]
    [Google Scholar]
  8. Kim H.-M. , Choi D.-H. , Hwang C.-Y. , Cho B. C. . ( 2008; ). Nocardioides salarius sp. nov., isolated from seawater enriched with zooplankton. . Int J Syst Evol Microbiol 58:, 2056–2064. [CrossRef] [PubMed]
    [Google Scholar]
  9. Kimura M. . ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef] [PubMed]
    [Google Scholar]
  10. Lawson P. A. , Collins M. D. , Schumann P. , Tindall B. J. , Hirsch P. , Labrenz M. . ( 2000; ). New ll-diaminopimelic acid-containing actinomycetes from hypersaline, heliothermal and meromictic Antarctic Ekho Lake: Nocardioides aquaticus sp. nov. and Friedmanniella lacustris sp. nov.. Syst Appl Microbiol 23:, 219–229. [CrossRef] [PubMed]
    [Google Scholar]
  11. Lee S. D. . ( 2007; ). Nocardioides furvisabuli sp. nov., isolated from black sand. . Int J Syst Evol Microbiol 57:, 35–39. [CrossRef] [PubMed]
    [Google Scholar]
  12. Lee S. D. , Lee D. W. , Kim J. S. . ( 2008; ). Nocardioides hwasunensis sp. nov.. Int J Syst Evol Microbiol 58:, 278–281. [CrossRef] [PubMed]
    [Google Scholar]
  13. Li B. , Xie C.-H. , Yokota A. . ( 2007; ). Nocardioides exalbidus sp. nov., a novel actinomycete isolated from lichen in Izu-Oshima Island, Japan. . Actinomycetologica 21:, 22–26. [CrossRef]
    [Google Scholar]
  14. MacKenzie S. L. . ( 1987; ). Gas chromatographic analysis of amino acids as the N-heptafluorobutyryl isobutyl esters. . J Assoc Off Anal Chem 70:, 151–160.[PubMed]
    [Google Scholar]
  15. Margesin R. . ( 2009; ). Effect of temperature on growth parameters of psychrophilic bacteria and yeasts. . Extremophiles 13:, 257–262. [CrossRef] [PubMed]
    [Google Scholar]
  16. Margesin R. , Gander S. , Zacke G. , Gounot A. M. , Schinner F. . ( 2003; ). Hydrocarbon degradation and enzyme activities of cold-adapted bacteria and yeasts. . Extremophiles 7:, 451–458. [CrossRef] [PubMed]
    [Google Scholar]
  17. Margesin R. , Schinner F. , Marx J. C. , Gerday C. . (editors) ( 2008; ). Psychrophiles: from Biodiversity to Biotechnology. Berlin, Heidelberg:: Springer;. [CrossRef]
    [Google Scholar]
  18. Marmur J. , Doty P. . ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. . J Mol Biol 5:, 109–118. [CrossRef] [PubMed]
    [Google Scholar]
  19. Morita R. Y. . ( 1975; ). Psychrophilic bacteria. . Bacteriol Rev 39:, 144–167.[PubMed]
    [Google Scholar]
  20. O’Donnell A. G. , Goodfellow M. , Minnikin D. E. . ( 1982; ). Lipids in the classification of Nocardioides: reclassification of Arthrobacter simplex (Jensen) lochhead in the genus Nocardioides (Prauser) emend. O’Donnell et al. as Nocardioides simplex comb. nov.. Arch Microbiol 133:, 323–329. [CrossRef] [PubMed]
    [Google Scholar]
  21. Prauser H. . ( 1976; ). Nocardioides, a new genus of the order Actinomycetales . . Int J Syst Bacteriol 26:, 58–65. [CrossRef]
    [Google Scholar]
  22. Reasoner D. J. , Geldreich E. E. . ( 1985; ). A new medium for the enumeration and subculture of bacteria from potable water. . Appl Environ Microbiol 49:, 1–7.[PubMed]
    [Google Scholar]
  23. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  24. Sambrook J. , Frisch E. F. , Maniatis T. . ( 1989; ). Molecular Cloning: a Laboratory Manual, , 2nd edn.. Cold Spring Harbor, NY:: Cold Spring Harbor Laboratory;.
    [Google Scholar]
  25. Sasser M. . ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc;.
  26. Schippers A. , Schumann P. , Spröer C. . ( 2005; ). Nocardioides oleivorans sp. nov., a novel crude-oil-degrading bacterium. . Int J Syst Evol Microbiol 55:, 1501–1504. [CrossRef] [PubMed]
    [Google Scholar]
  27. Schleifer K. H. . ( 1985; ). Analysis of the chemical composition and primary structure of murein. . Methods Microbiol 18:, 123–156. [CrossRef]
    [Google Scholar]
  28. Schleifer K. H. , Kandler O. . ( 1972; ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. . Bacteriol Rev 36:, 407–477.[PubMed]
    [Google Scholar]
  29. Staneck J. L. , Roberts G. D. . ( 1974; ). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. . Appl Microbiol 28:, 226–231.[PubMed]
    [Google Scholar]
  30. Tamura K. , Dudley J. , Nei M. , Kumar S. . ( 2007; ). mega4: Molecular Evolutionary Genetics Analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef] [PubMed]
    [Google Scholar]
  31. Thompson J. D. , Gibson T. J. , Plewniak F. , Jeanmougin F. , Higgins D. G. . ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef] [PubMed]
    [Google Scholar]
  32. Tindall B. J. . ( 1990a; ). Lipid composition of Halobacterium lacusprofundi . . FEMS Microbiol Lett 66:, 199–202. [CrossRef]
    [Google Scholar]
  33. Tindall B. J. . ( 1990b; ). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. . Syst Appl Microbiol 13:, 128–130.[CrossRef]
    [Google Scholar]
  34. Tóth E. M. , Kéki Z. , Homonnay Z. G. , Borsodi A. K. , Márialigeti K. , Schumann P. . ( 2008; ). Nocardioides daphniae sp. nov., isolated from Daphnia cucullata (Crustacea: Cladocera). . Int J Syst Evol Microbiol 58:, 78–83. [CrossRef] [PubMed]
    [Google Scholar]
  35. Urzì C. , Salamone P. , Schumann P. , Stackebrandt E. . ( 2000; ). Marmoricola aurantiacus gen. nov., sp. nov., a coccoid member of the family Nocardioidaceae isolated from a marble statue. . Int J Syst Evol Microbiol 50:, 529–536. [CrossRef] [PubMed]
    [Google Scholar]
  36. Whiton R. S. , Lau P. , Morgan S. L. , Gilbart J. , Fox A. . ( 1985; ). Modifications in the alditol acetate method for analysis of muramic acid and other neutral and amino sugars by capillary gas chromatography-mass spectrometry with selected ion monitoring. . J Chromatogr A 347:, 109–120. [CrossRef] [PubMed]
    [Google Scholar]
  37. Wu C. , Lu X. , Qin M. , Wang Y. , Ruan J. . ( 1989; ). Analysis of menaquinone compound in microbial cells by HPLC. . Microbiology [English translation of Microbiology (Beijing)] 16:, 176–178.
    [Google Scholar]
  38. Yi H. , Chun J. . ( 2004; ). Nocardioides ganghwensis sp. nov., isolated from tidal flat sediment. . Int J Syst Evol Microbiol 54:, 1295–1299. [CrossRef] [PubMed]
    [Google Scholar]
  39. Yoon J.-H. , Kim I.-G. , Kang K. H. , Oh T.-K. , Park Y.-H. . ( 2004; ). Nocardioides aquiterrae sp. nov., isolated from groundwater in Korea. . Int J Syst Evol Microbiol 54:, 71–75. [CrossRef] [PubMed]
    [Google Scholar]
  40. Yoon J.-H. , Lee J.-K. , Jung S.-Y. , Kim J.-A. , Kim H.-K. , Oh T.-K. . ( 2006; ). Nocardioides kongjuensis sp. nov., an N-acylhomoserine lactone-degrading bacterium. . Int J Syst Evol Microbiol 56:, 1783–1787. [CrossRef] [PubMed]
    [Google Scholar]
  41. Yoon J.-H. , Kang S.-J. , Lee C.-H. , Oh T.-K. . ( 2007; ). Nocardioides insulae sp. nov., isolated from soil. . Int J Syst Evol Microbiol 57:, 136–140. [CrossRef] [PubMed]
    [Google Scholar]
  42. Yoon J.-H. , Kang S.-J. , Park S. Y. , Kim W. Y. , Oh T.-K. . ( 2009; ). Nocardioides caeni sp. nov., isolated from wastewater. . Int J Syst Evol Microbiol 59:, 2794–2797. [CrossRef] [PubMed]
    [Google Scholar]
  43. Zhang J.-Y. , Liu X.-Y. , Liu S.-J. . ( 2009; ). Nocardioides terrae sp. nov., isolated from forest soil. . Int J Syst Evol Microbiol 59:, 2444–2448. [CrossRef] [PubMed]
    [Google Scholar]
  44. Zhang D.-C. , Redzic M. , Schinner F. , Margesin R. . ( 2011; ). Glaciimonas immobilis gen. nov., sp. nov., a member of the family Oxalobacteraceae isolated from alpine glacier cryoconite. . Int J Syst Evol Microbiol 61:, 2186–2190.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.031047-0
Loading
/content/journal/ijsem/10.1099/ijs.0.031047-0
Loading

Data & Media loading...

Supplements

Supplementary material 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error