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Abstract

A novel alphaproteobacterial strain, designated CPCC 100156, was isolated from a forest soil sample collected from Hainan Island, South China, and subjected to taxonomic investigation using a polyphasic approach. The pink- to rosy-coloured colonies on TSA and YM agar were smooth and moist. Good growth occurred at 28–32 °C and at pH 7.0–7.5. The respiratory quinone was ubiquinone-9. The polar lipids consisted of phosphatidylcholine (PC), hydroxyphosphatidylethanolamine (OH-PE), phosphatidylglycerol (PG), diphosphatidylglycerol (DPG) and two unidentified aminolipids (AL1, AL2), with a minor amount of ninhydrin-positive phosphoglycolipid. (NPG). The major cellular fatty acids were summed feature 8 (Cω7 /Cω6) (49.5 %), summed feature 3 (Cω7/Cω6) (22.5 %), and C (14.0 %). The G+C content of the genomic DNA was 70.3 mol%. The organism showed 16S rRNA gene sequence similarity of 97.37 % with DSM 16746. Phylogenetic analyses based on 16S rRNA gene sequences showed that the isolate belonged to the family and consistently formed a robust cluster with DSM 16746 in the phylogenetic tree. The DNA–DNA hybridization value between the new isolate and DSM 16746 was 45.6 %. On the basis of the taxonomic evidence, it is proposed that strain CPCC 100156 represents a novel species, for which the name sp. nov. is proposed. The type strain is CPCC 100156 ( = DSM 23312 = CGMCC 1.10758). The description of the genus is emended accordingly.

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2012-03-01
2019-09-16
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