1887

Abstract

A novel alphaproteobacterial strain, designated CPCC 100156, was isolated from a forest soil sample collected from Hainan Island, South China, and subjected to taxonomic investigation using a polyphasic approach. The pink- to rosy-coloured colonies on TSA and YM agar were smooth and moist. Good growth occurred at 28–32 °C and at pH 7.0–7.5. The respiratory quinone was ubiquinone-9. The polar lipids consisted of phosphatidylcholine (PC), hydroxyphosphatidylethanolamine (OH-PE), phosphatidylglycerol (PG), diphosphatidylglycerol (DPG) and two unidentified aminolipids (AL1, AL2), with a minor amount of ninhydrin-positive phosphoglycolipid. (NPG). The major cellular fatty acids were summed feature 8 (Cω7 /Cω6) (49.5 %), summed feature 3 (Cω7/Cω6) (22.5 %), and C (14.0 %). The G+C content of the genomic DNA was 70.3 mol%. The organism showed 16S rRNA gene sequence similarity of 97.37 % with DSM 16746. Phylogenetic analyses based on 16S rRNA gene sequences showed that the isolate belonged to the family and consistently formed a robust cluster with DSM 16746 in the phylogenetic tree. The DNA–DNA hybridization value between the new isolate and DSM 16746 was 45.6 %. On the basis of the taxonomic evidence, it is proposed that strain CPCC 100156 represents a novel species, for which the name sp. nov. is proposed. The type strain is CPCC 100156 ( = DSM 23312 = CGMCC 1.10758). The description of the genus is emended accordingly.

Funding
This study was supported by the:
  • National Facilities and Information Infrastructure for Science and Technology (Award 2005DKA21203)
  • National S&T Major Special Project on Major New Drug Innovation (Award 2009ZX09301-003 and 2009ZX09302-004)
  • National Basic Research Program of China (Award 2010CB833800)
  • National Natural Science Foundation of China (Award 30870026, 30970008 and 30970038)
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2012-03-01
2021-04-21
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References

  1. Brunisholz R. A., Suter F., Zuber H. 1994; Structural and spectral characterisation of the antenna complexes of Rhodocyclus gelatinosus. Indications of a hairpin-like-arranged antenna apoprotein with an unusually high alanine content. Eur J Biochem 222:667–675 [CrossRef][PubMed]
    [Google Scholar]
  2. Bryantseva I. A., Gorlenko V. M., Kompantseva E. I., Imhoff J. F. 2000; Thioalkalicoccus limnaeus gen. nov., sp. nov., a new alkaliphilic purple sulfur bacterium with bacteriochlorophyll b . Int J Syst Evol Microbiol 50:2157–2163 [CrossRef][PubMed]
    [Google Scholar]
  3. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [CrossRef][PubMed]
    [Google Scholar]
  4. Felsenstein J. 1985; Conference limits on phylogenies: an approach using the bootstrap. Evolution 39:783–789 [CrossRef]
    [Google Scholar]
  5. Hiraishi A., Matsuzawa Y., Kanbe T., Wakao N. 2000; Acidisphaera rubrifaciens gen. nov., sp. nov., an aerobic bacteriochlorophyll-containing bacterium isolated from acidic environments. Int J Syst Evol Microbiol 50:1539–1546 [CrossRef][PubMed]
    [Google Scholar]
  6. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef][PubMed]
    [Google Scholar]
  7. Kimura M. 1983 The Neutral Theory of Molecular Evolution Cambridge: Cambridge University Press; [CrossRef]
    [Google Scholar]
  8. Kroppenstedt R. M. 1985; Fatty acid and menaquinone analysis of actinomycetes and related organisms. In Chemical Methods in Bacterial Systematics (Society for Applied Bacteriology Technical Series vol. 20) pp. 173–199 Edited by Goodfellow M., Minnikin D. E. New York: Academic Press;
    [Google Scholar]
  9. Lee J. S., Shin Y. K., Yoon J. H., Takeuchi M., Pyun Y. R., Park Y. H. 2001; Sphingomonas aquatilis sp. nov., Sphingomonas koreensis sp. nov., and Sphingomonas taejonensis sp. nov., yellow-pigmented bacteria isolated from natural mineral water. Int J Syst Evol Microbiol 51:1491–1498[PubMed]
    [Google Scholar]
  10. Li W. J., Xu P., Schumann P., Zhang Y. Q., Pukall R., Xu L. H., Stackebrandt E., Jiang C. L. 2007; Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia . Int J Syst Evol Microbiol 57:1424–1428 [CrossRef][PubMed]
    [Google Scholar]
  11. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [CrossRef][PubMed]
    [Google Scholar]
  12. Meier A., Kirschner P., Schröder K. H., Wolters J., Kroppenstedt R. M., Böttger E. C. 1993; Mycobacterium intermedium sp. nov.. Int J Syst Bacteriol 43:204–209 [CrossRef][PubMed]
    [Google Scholar]
  13. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [CrossRef]
    [Google Scholar]
  14. Rainey F. A., Silva J., Nobre M. F., Silva M. T., da Costa M. S. 2003; Porphyrobacter cryptus sp. nov., a novel slightly thermophilic, aerobic, bacteriochlorophyll a-containing species. Int J Syst Evol Microbiol 53:35–41 [CrossRef][PubMed]
    [Google Scholar]
  15. Reddy G. S., Nagy M., Garcia-Pichel F. 2006; Belnapia moabensis gen. nov., sp. nov., an alphaproteobacterium from biological soil crusts in the Colorado Plateau, USA. Int J Syst Evol Microbiol 56:51–58 [CrossRef][PubMed]
    [Google Scholar]
  16. Saitoh S., Suzuki T., Nishimura Y. 1998; Proposal of Craurococcus roseus gen. nov., sp. nov. and Paracraurococcus ruber gen. nov., sp. nov., novel aerobic bacteriochlorophyll a-containing bacteria from soil. Int J Syst Bacteriol 48:1043–1047 [CrossRef][PubMed]
    [Google Scholar]
  17. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  18. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp. 607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  19. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [CrossRef][PubMed]
    [Google Scholar]
  20. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef][PubMed]
    [Google Scholar]
  21. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International committee on systematic bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  22. Xu P., Li W. J., Tang S. K., Zhang Y. Q., Chen G. Z., Chen H. H., Xu L. H., Jiang C. L. 2005; Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family ‘Oxalobacteraceae’ isolated from China. Int J Syst Evol Microbiol 55:1149–1153 [CrossRef][PubMed]
    [Google Scholar]
  23. Zhang Y. Q., Yu L. Y., Wang D., Liu H. Y., Sun C. H., Jiang W., Zhang Y. Q., Li W. J. 2008; Roseomonas vinacea sp. nov., a Gram-negative coccobacillus isolated from a soil sample. Int J Syst Evol Microbiol 58:2070–2074 [CrossRef][PubMed]
    [Google Scholar]
  24. Zhi X. Y., Li W. J., Stackebrandt E. 2009; An update of the structure and 16S rRNA gene sequence-based definition of higher ranks of the class Actinobacteria, with the proposal of two new suborders and four new families and emended descriptions of the existing higher taxa. Int J Syst Evol Microbiol 59:589–608 [CrossRef][PubMed]
    [Google Scholar]
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