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Volume 62, Issue Pt_5

sp. nov., a moderate halophile isolated from Keke Salt Lake Free

Abstract

A novel moderately halophilic bacterium, designated strain K170, was isolated from Keke Salt Lake in Qinghai, China. The strain grew with 0–22 % (w/v) NaCl, at 4–50 °C and at pH 6–11, with optimum growth in 3 % (w/v) NaCl, at 40 °C and at pH 8. The predominant respiratory quinone was menaquinone 7 (MK-7). The polar lipids included diphosphatidylglycerol, phosphatidylglycerol, unidentified phospholipids, aminolipids and glycolipids. The major cellular fatty acids were anteiso-C, iso-C and anteiso-C. The DNA G+C content was 35.8 mol%. Phylogenetic analysis based on the full-length 16S rRNA gene sequence revealed that strain K170 was a member of the genus High levels of 16S rRNA gene sequence similarity were found between strain K170 and DSM 17256 (97.3 %) and JCM 15569 (97.1 %). 16S rRNA gene sequence similarities between strain K170 and the type strains of other recognized members of the genus were below 97 %. The DNA–DNA hybridization values of strain K170 with DSM 17256 and JCM 15569 were 21.9 % and 34.3 %, respectively. On the basis of these results, strain K170 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed; the type strain is K170 ( = CGMCC 1.10681 = DSM 23178).

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Funding
This study was supported by the:
  • genetically modified organisms breeding major projects (Award 2009ZX08009)
© 2012 IUMS
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2012-05-01
2024-12-08
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Gracilibacillus kekensis sp. nov., a moderate halophile isolated from Keke Salt Lake
Int J Syst Evol Microbiol 62, 1032 (2012); https://doi.org/10.1099/ijs.0.030858-0
/content/journal/ijsem/10.1099/ijs.0.030858-0
/content/journal/ijsem/10.1099/ijs.0.030858-0
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