1887

Abstract

A novel moderately halophilic bacterium, designated strain K170, was isolated from Keke Salt Lake in Qinghai, China. The strain grew with 0–22 % (w/v) NaCl, at 4–50 °C and at pH 6–11, with optimum growth in 3 % (w/v) NaCl, at 40 °C and at pH 8. The predominant respiratory quinone was menaquinone 7 (MK-7). The polar lipids included diphosphatidylglycerol, phosphatidylglycerol, unidentified phospholipids, aminolipids and glycolipids. The major cellular fatty acids were anteiso-C, iso-C and anteiso-C. The DNA G+C content was 35.8 mol%. Phylogenetic analysis based on the full-length 16S rRNA gene sequence revealed that strain K170 was a member of the genus High levels of 16S rRNA gene sequence similarity were found between strain K170 and DSM 17256 (97.3 %) and JCM 15569 (97.1 %). 16S rRNA gene sequence similarities between strain K170 and the type strains of other recognized members of the genus were below 97 %. The DNA–DNA hybridization values of strain K170 with DSM 17256 and JCM 15569 were 21.9 % and 34.3 %, respectively. On the basis of these results, strain K170 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed; the type strain is K170 ( = CGMCC 1.10681 = DSM 23178).

Funding
This study was supported by the:
  • , genetically modified organisms breeding major projects , (Award 2009ZX08009)
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2012-05-01
2020-08-12
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References

  1. Ahmed I., Yokota A., Fujiwara T. 2007; Gracilibacillus boraciitolerans sp. nov., a highly boron-tolerant and moderately halotolerant bacterium isolated from soil. Int J Syst Evol Microbiol 57:796–802 [CrossRef][PubMed]
    [Google Scholar]
  2. Atlas R. M. 1993 Handbook of Microbiological Media Edited by Parks L. C. Boca Raton, FL: CRC Press;
    [Google Scholar]
  3. Barrow G. I., Feltham R. K. A. (editors) 1993 Cowan and Steel’s Manual for the Identification of Medical Bacteria, 3rd edn. Cambridge: Cambridge University Press; [CrossRef]
    [Google Scholar]
  4. Carrasco I. J., Márquez M. C., Yanfen X., Ma Y., Cowan D. A., Jones B. E., Grant W. D., Ventosa A. 2006; Gracilibacillus orientalis sp. nov., a novel moderately halophilic bacterium isolated from a salt lake in Inner Mongolia, China. Int J Syst Evol Microbiol 56:599–604 [CrossRef][PubMed]
    [Google Scholar]
  5. Chamroensaksri N., Tanasupawat S., Akaracharanya A., Visessanguan W., Kudo T., Itoh T. 2010; Gracilibacillus thailandensis sp. nov., from fermented fish (pla-ra). Int J Syst Evol Microbiol 60:944–948 [CrossRef][PubMed]
    [Google Scholar]
  6. Chen Y. G., Cui X. L., Zhang Y. Q., Li W. J., Wang Y. X., Xu L. H., Peng Q., Wen M. L., Jiang C. L. 2008a; Gracilibacillus halophilus sp. nov., a moderately halophilic bacterium isolated from saline soil. Int J Syst Evol Microbiol 58:2403–2408 [CrossRef][PubMed]
    [Google Scholar]
  7. Chen Y. G., Cui X. L., Zhang Y. Q., Li W. J., Wang Y. X., Xu L. H., Peng Q., Wen M. L., Jiang C. L. 2008b; Gracilibacillus quinghaiensis sp. nov., isolated from salt-lake sediment in the Qaidam Basin, north-west China. Syst Appl Microbiol 31:183–189 [CrossRef][PubMed]
    [Google Scholar]
  8. Collins M., Jones D. 1980; Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4-diaminobutyric acid. J Appl Microbiol 48:459–470 [CrossRef]
    [Google Scholar]
  9. Gregersen T. 1978; Rapid method for distinction of Gram-negative from Gram-positive bacteria. Eur J Appl Microbiol Biotechnol 5:123–127 [CrossRef]
    [Google Scholar]
  10. Harrigan W., McCance M. 1976 Laboratory Methods in Foods and Dairy Microbiology London: Academic Press;
    [Google Scholar]
  11. Hasegawa T., Takizawa M., Tanida S. 1983; A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29:319–322 [CrossRef]
    [Google Scholar]
  12. Heimbrook M. E., Wang W. L. L., Campbell G. 1989; Staining bacterial flagella easily. J Clin Microbiol 27:2612–2615[PubMed]
    [Google Scholar]
  13. Huo Y. Y., Xu X. W., Cui H. L., Wu M. 2010; Gracilibacillus ureilyticus sp. nov., a halotolerant bacterium from a saline-alkaline soil. Int J Syst Evol Microbiol 60:1383–1386 [CrossRef][PubMed]
    [Google Scholar]
  14. Jeon C. O., Lim J. M., Jang H. H., Park D. J., Xu L. H., Jiang C. L., Kim C. J. 2008; Gracilibacillus lacisalsi sp. nov., a halophilic Gram-positive bacterium from a salt lake in China. Int J Syst Evol Microbiol 58:2282–2286 [CrossRef][PubMed]
    [Google Scholar]
  15. Lawson P. A., Deutch C. E., Collins M. D. 1996; Phylogenetic characterization of a novel salt-tolerant Bacillus species: description of Bacillus dipsosauri sp. nov.. J Appl Bacteriol 81:109–112 [CrossRef][PubMed]
    [Google Scholar]
  16. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [CrossRef]
    [Google Scholar]
  17. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.;
  18. Sehgal S. N., Gibbons N. E. 1960; Effect of some metal ions on the growth of Halobacterium cutirubrum . Can J Microbiol 6:165–169 [CrossRef][PubMed]
    [Google Scholar]
  19. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Manual of Methods for General and Microbiology Bacteriology pp. 607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  20. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
    [Google Scholar]
  21. Tang S. K., Wang Y., Lou K., Mao P. H., Jin X., Jiang C. L., Xu L. H., Li W. J. 2009; Gracilibacillus saliphilus sp. nov., a moderately halophilic bacterium isolated from a salt lake. Int J Syst Evol Microbiol 59:1620–1624 [CrossRef][PubMed]
    [Google Scholar]
  22. Ventosa A., Quesada E., Rodriguez-Valera F., Ruiz-Berraquero F., Ramos-Cormenzana A. 1982; Numerical taxonomy of moderately halophilic Gram-negative rods. J Gen Microbiol 128:1959–1968
    [Google Scholar]
  23. Wainø M., Tindall B. J., Schumann P., Ingvorsen K. 1999; Gracilibacillus gen. nov., with description of Gracilibacillus halotolerans gen. nov., sp. nov.; transfer of Bacillus dipsosauri to Gracilibacillus dipsosauri comb. nov., and Bacillus salexigens to the genus Salibacillus gen. nov., as Salibacillus salexigens comb. nov.. Int J Syst Bacteriol 49:821–831 [CrossRef][PubMed]
    [Google Scholar]
  24. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  25. Yanagi M., Yamasato K. 1993; Phylogenetic analysis of the family Rhizobiaceae and related bacteria by sequencing of 16S rRNA gene using PCR and DNA sequencer. FEMS Microbiol Lett 107:115–120 [CrossRef][PubMed]
    [Google Scholar]
  26. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H. 1996; Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46:502–505 [CrossRef]
    [Google Scholar]
  27. Ziemke F., Höfle M. G., Lalucat J., Rosselló-Mora R. 1998; Reclassification of Shewanella putrefaciens Owen’s genomic group II as Shewanella baltica sp. nov.. Int J Syst Bacteriol 48:179–186 [CrossRef][PubMed]
    [Google Scholar]
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