1887

Abstract

Three Gram-stain-positive strains were isolated from fermented mustard and were rod-shaped, non-motile, asporogenous, facultatively anaerobic, homofermentative and did not exhibit catalase activity. Comparative analyses of 16S rRNA, and gene sequences demonstrated that the novel strains were members of the genus . On the basis of 16S rRNA gene sequence analysis, the type strains of (98.7 % similarity), (98.9 %) and (97.9 %) were the closest neighbours. However, DNA–DNA reassociation values with these strains were less than 50 %. Phenotypic and genotypic features demonstrated that these isolates represent a novel species of the genus , for which the name sp. nov. is proposed; the type strain is YM 0097 ( = JCM 17355 = BCRC 80278).

Funding
This study was supported by the:
  • National Science Council, Taiwan (Award NSC 95-2313-B-010-001-MY3)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.030619-0
2012-03-01
2024-12-12
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/3/489.html?itemId=/content/journal/ijsem/10.1099/ijs.0.030619-0&mimeType=html&fmt=ahah

References

  1. Barrow G. I., Feltham R. K. A. (editors) 1993 Cowan and Steel’s Manual for the Identification of Medical Bacteria, 3rd edn. Cambridge: Cambridge University Press; [View Article]
    [Google Scholar]
  2. Chao S.-H., Tomii Y., Sasamoto M., Fujimoto J., Tsai Y. C., Watanabe K. 2008; Lactobacillus capillatus sp. nov., a motile bacterium isolated from stinky tofu brine. Int J Syst Evol Microbiol 58:2555–2559 [View Article][PubMed]
    [Google Scholar]
  3. Chao S.-H., Wu R. J., Watanabe K., Tsai Y.-C. 2009; Diversity of lactic acid bacteria in suan-tsai and fu-tsai, traditional fermented mustard products of Taiwan. Int J Food Microbiol 135:203–210 [View Article][PubMed]
    [Google Scholar]
  4. de Man J. D., Rogosa M., Sharpe M. E. 1960; A medium for the cultivation of lactobacilli. J Appl Bacteriol 23:130–135 [View Article]
    [Google Scholar]
  5. Ehrmann M. A., Müller M. R., Vogel R. F. 2003; Molecular analysis of sourdough reveals Lactobacillus mindensis sp. nov.. Int J Syst Evol Microbiol 53:7–13 [View Article][PubMed]
    [Google Scholar]
  6. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determined genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [View Article]
    [Google Scholar]
  7. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
    [Google Scholar]
  8. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [View Article]
    [Google Scholar]
  9. Kandler O., Weiss N. 1986; Genus Lactobacillus . In Bergey’s Manual of Systematic Bacteriology vol. 2 pp. 1063–1065 Edited by Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  10. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [View Article]
    [Google Scholar]
  11. Kuykendall L. D., Roy M. A., O'Neill J. J., Devine T. E. 1988; Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum . Int J Syst Bacteriol 38:358–361 [View Article]
    [Google Scholar]
  12. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganism. J Mol Biol 3:208–218 [View Article]
    [Google Scholar]
  13. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
    [Google Scholar]
  14. Miller L. T. 1982; Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. J Clin Microbiol 16:584–586[PubMed]
    [Google Scholar]
  15. Naser S. M., Thompson F. L., Hoste B., Gevers D., Dawyndt P., Vancanneyt M., Swings J. 2005; Application of multilocus sequence analysis (MLSA) for rapid identification of Enterococcus species based on rpoA and pheS genes. Microbiology 151:2141–2150 [View Article][PubMed]
    [Google Scholar]
  16. Naser S. M., Dawyndt P., Hoste B., Gevers D., Vandemeulebroecke K., Cleenwerck I., Vancanneyt M., Swings J. 2007; Identification of lactobacilli by pheS and rpoA gene sequence analyses. Int J Syst Evol Microbiol 57:2777–2789 [View Article][PubMed]
    [Google Scholar]
  17. Reuter G. 1983; Lactobacillus alimentarius sp. nov., nom. rev. and Lactobacillus farciminis sp. nov., nom. rev.. Syst Appl Microbiol 4:277–279 [CrossRef]
    [Google Scholar]
  18. Rzhetsky A., Nei M. 1992; A simple method for estimating and testing minimum-evolution trees. Mol Biol Evol 9:945–967
    [Google Scholar]
  19. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  20. Scheirlinck I., Van der Meulen R., Van Schoor A., Huys G., Vandamme P., De Vuyst L., Vancanneyt M. 2007; Lactobacillus crustorum sp. nov., isolated from two traditional Belgian wheat sourdoughs. Int J Syst Evol Microbiol 57:1461–1467 [View Article][PubMed]
    [Google Scholar]
  21. Schleifer K. H., Kandler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–477[PubMed]
    [Google Scholar]
  22. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [View Article]
    [Google Scholar]
  23. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega 4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [View Article][PubMed]
    [Google Scholar]
  24. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
    [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.030619-0
Loading
/content/journal/ijsem/10.1099/ijs.0.030619-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error