1887

Abstract

Three Gram-stain-positive strains were isolated from fermented mustard and were rod-shaped, non-motile, asporogenous, facultatively anaerobic, homofermentative and did not exhibit catalase activity. Comparative analyses of 16S rRNA, and gene sequences demonstrated that the novel strains were members of the genus . On the basis of 16S rRNA gene sequence analysis, the type strains of (98.7 % similarity), (98.9 %) and (97.9 %) were the closest neighbours. However, DNA–DNA reassociation values with these strains were less than 50 %. Phenotypic and genotypic features demonstrated that these isolates represent a novel species of the genus , for which the name sp. nov. is proposed; the type strain is YM 0097 ( = JCM 17355 = BCRC 80278).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.030619-0
2012-03-01
2019-09-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/3/489.html?itemId=/content/journal/ijsem/10.1099/ijs.0.030619-0&mimeType=html&fmt=ahah

References

  1. Barrow G. I. , Feltham R. K. A. . (editors) ( 1993; ). Cowan and Steel’s Manual for the Identification of Medical Bacteria, , 3rd edn.. Cambridge:: Cambridge University Press;. [CrossRef]
    [Google Scholar]
  2. Chao S.-H. , Tomii Y. , Sasamoto M. , Fujimoto J. , Tsai Y. C. , Watanabe K. . ( 2008; ). Lactobacillus capillatus sp. nov., a motile bacterium isolated from stinky tofu brine. . Int J Syst Evol Microbiol 58:, 2555–2559. [CrossRef] [PubMed]
    [Google Scholar]
  3. Chao S.-H. , Wu R. J. , Watanabe K. , Tsai Y.-C. . ( 2009; ). Diversity of lactic acid bacteria in suan-tsai and fu-tsai, traditional fermented mustard products of Taiwan. . Int J Food Microbiol 135:, 203–210. [CrossRef] [PubMed]
    [Google Scholar]
  4. de Man J. D. , Rogosa M. , Sharpe M. E. . ( 1960; ). A medium for the cultivation of lactobacilli. . J Appl Bacteriol 23:, 130–135. [CrossRef]
    [Google Scholar]
  5. Ehrmann M. A. , Müller M. R. , Vogel R. F. . ( 2003; ). Molecular analysis of sourdough reveals Lactobacillus mindensis sp. nov.. Int J Syst Evol Microbiol 53:, 7–13. [CrossRef] [PubMed]
    [Google Scholar]
  6. Ezaki T. , Hashimoto Y. , Yabuuchi E. . ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determined genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39:, 224–229. [CrossRef]
    [Google Scholar]
  7. Felsenstein J. . ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  8. Fitch W. M. . ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20:, 406–416. [CrossRef]
    [Google Scholar]
  9. Kandler O. , Weiss N. . ( 1986; ). Genus Lactobacillus . . In Bergey’s Manual of Systematic Bacteriology, vol. 2, pp. 1063–1065. Edited by Sneath P. H. A. , Mair N. S. , Sharpe M. E. , Holt J. G. . . Baltimore:: Williams & Wilkins;.
    [Google Scholar]
  10. Kimura M. . ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef]
    [Google Scholar]
  11. Kuykendall L. D. , Roy M. A. , O'Neill J. J. , Devine T. E. . ( 1988; ). Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum . . Int J Syst Bacteriol 38:, 358–361. [CrossRef]
    [Google Scholar]
  12. Marmur J. . ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from microorganism. . J Mol Biol 3:, 208–218. [CrossRef]
    [Google Scholar]
  13. Mesbah M. , Premachandran U. , Whitman W. B. . ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  14. Miller L. T. . ( 1982; ). Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. . J Clin Microbiol 16:, 584–586.[PubMed]
    [Google Scholar]
  15. Naser S. M. , Thompson F. L. , Hoste B. , Gevers D. , Dawyndt P. , Vancanneyt M. , Swings J. . ( 2005; ). Application of multilocus sequence analysis (MLSA) for rapid identification of Enterococcus species based on rpoA and pheS genes. . Microbiology 151:, 2141–2150. [CrossRef] [PubMed]
    [Google Scholar]
  16. Naser S. M. , Dawyndt P. , Hoste B. , Gevers D. , Vandemeulebroecke K. , Cleenwerck I. , Vancanneyt M. , Swings J. . ( 2007; ). Identification of lactobacilli by pheS and rpoA gene sequence analyses. . Int J Syst Evol Microbiol 57:, 2777–2789. [CrossRef] [PubMed]
    [Google Scholar]
  17. Reuter G. . ( 1983; ). Lactobacillus alimentarius sp. nov., nom. rev. and Lactobacillus farciminis sp. nov., nom. rev.. Syst Appl Microbiol 4:, 277–279.[CrossRef]
    [Google Scholar]
  18. Rzhetsky A. , Nei M. . ( 1992; ). A simple method for estimating and testing minimum-evolution trees. . Mol Biol Evol 9:, 945–967.
    [Google Scholar]
  19. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  20. Scheirlinck I. , Van der Meulen R. , Van Schoor A. , Huys G. , Vandamme P. , De Vuyst L. , Vancanneyt M. . ( 2007; ). Lactobacillus crustorum sp. nov., isolated from two traditional Belgian wheat sourdoughs. . Int J Syst Evol Microbiol 57:, 1461–1467. [CrossRef] [PubMed]
    [Google Scholar]
  21. Schleifer K. H. , Kandler O. . ( 1972; ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. . Bacteriol Rev 36:, 407–477.[PubMed]
    [Google Scholar]
  22. Stackebrandt E. , Goebel B. M. . ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44:, 846–849. [CrossRef]
    [Google Scholar]
  23. Tamura K. , Dudley J. , Nei M. , Kumar S. . ( 2007; ). mega 4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef] [PubMed]
    [Google Scholar]
  24. Thompson J. D. , Gibson T. J. , Plewniak F. , Jeanmougin F. , Higgins D. G. . ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.030619-0
Loading
/content/journal/ijsem/10.1099/ijs.0.030619-0
Loading

Data & Media loading...

Supplementary material 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error