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Abstract

A novel Gram-stain-positive, white-pigmented, non-motile, non-sporulating, catalase- and oxidase-positive, strictly aerobic coccus, designated strain ZXM223, was isolated from a seawater sample collected from the coast of Qingdao, PR China, during a green algal bloom. It grew at pH 6.0–10.5 and 0–25.0 % (w/v) NaCl, with optimum growth at pH 8.5 and 3.0 % (w/v) NaCl. Growth occurred at 16–42 °C (optimum at 28 °C). The major fatty acids were anteiso-C and iso-C. Menaquinone 6 (MK-6) was the major respiratory quinone. The polar lipids were phosphatidylglycerol, three unidentified phospholipids and two unknown glycolipids. The peptidoglycan type was -Lys–Gly The genomic DNA G+C content was 43.5 mol%. Phylogenetic analysis of the 16S rRNA gene sequence placed strain ZXM223 within the genus , with sequence similarity of 92.2–97.1 % between ZXM223 and the type strains of this genus. The closest relatives were YIM Y15, ‘’ YIM-C678 and . T8. The DNA–DNA relatedness between strain ZXM223 and CGMCC 1.6302 and ‘’ CGMCC 1.6299 ( = YIM-C678) was 37±3 and 30±2 %, respectively. The phenotypic, chemotaxonomic and phylogenetic characteristics and low DNA–DNA relatedness support the proposal of a novel species of the genus , sp. nov., with the type strain ZXM223 ( = LMG 24855  = CGMCC 1.8895).

Funding
This study was supported by the:
  • National High Technology R&D Program of China (Award 2007AA09Z434)
  • National Natural Science Foundation of China (Award 40876067)
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2012-03-01
2021-10-15
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References

  1. Amoozegar M. A., Schumann P., Hajighasemi M., Ashengroph M., Razavi M. R. 2008; Salinicoccus iranensis sp. nov., a novel moderate halophile. Int J Syst Evol Microbiol 58:178–183 [View Article][PubMed]
    [Google Scholar]
  2. Aslam Z., Lim J. H., Im W.-T., Yasir M., Chung Y. R., Lee S.-T. 2007; Salinicoccus jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 57:633–638 [View Article][PubMed]
    [Google Scholar]
  3. Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. (editors) 1995 Short Protocols in Molecular Biology: a Compendium of Methods from Current Protocols in Molecular Biology, 3rd edn. pp. 211–214 New York: Wiley;
    [Google Scholar]
  4. Beveridge T. J., Lawrence J. R., Murray R. G. E. 2007; Sampling and staining for light microscopy. In Methods for General and Molecular Microbiology pp. 19–33 Edited by Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G., Schmidt T. M., Snyder L. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  5. Chen Y.-G., Cui X.-L., Pukall R., Li H.-M., Yang Y.-L., Xu L.-H., Wen M.-L., Peng Q., Jiang C.-L. 2007; Salinicoccus kunmingensis sp. nov., a moderately halophilic bacterium isolated from a salt mine in Yunnan, south-west China. Int J Syst Evol Microbiol 57:2327–2332 [View Article][PubMed]
    [Google Scholar]
  6. Chen Y.-G., Cui X.-L., Li W.-J., Xu L.-H., Wen M.-L., Peng Q., Jiang C.-L. 2008; Salinicoccus salitudinis sp. nov., a new moderately halophilic bacterium isolated from a saline soil sample. Extremophiles 12:197–203 [View Article][PubMed]
    [Google Scholar]
  7. Chen Y.-G., Cui X.-L., Wang Y.-X., Zhang Y.-Q., Li Q.-Y., Liu Z.-X., Wen M.-L., Peng Q., Li W.-J. 2009; Salinicoccus albus sp. nov., a halophilic bacterium from a salt mine. Int J Syst Evol Microbiol 59:874–879 [View Article][PubMed]
    [Google Scholar]
  8. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [View Article][PubMed]
    [Google Scholar]
  9. França L., Rainey F. A., Nobre M. F., da Costa M. S. 2006; Salinicoccus salsiraiae sp. nov.: a new moderately halophilic gram-positive bacterium isolated from salted skate. Extremophiles 10:531–536 [View Article][PubMed]
    [Google Scholar]
  10. Jung M. J., Kim M. S., Roh S. W., Shin K. S., Bae J. W. 2010; Salinicoccus carnicancri sp. nov., a halophilic bacterium isolated from a Korean fermented seafood. Int J Syst Evol Microbiol 60:653–658 [View Article][PubMed]
    [Google Scholar]
  11. Liu C., Shao Z. 2005; Alcanivorax dieselolei sp. nov., a novel alkane-degrading bacterium isolated from sea water and deep-sea sediment. Int J Syst Evol Microbiol 55:1181–1186 [View Article][PubMed]
    [Google Scholar]
  12. MacKenzie S. L. 1987; Gas chromatographic analysis of amino acids as the N-heptafluorobutyryl isobutyl esters. J Assoc Off Anal Chem 70:151–160[PubMed]
    [Google Scholar]
  13. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
    [Google Scholar]
  14. Ostle A. G., Holt J. G. 1982; Nile blue A as a fluorescent stain for poly-β-hydroxybutyrate. Appl Environ Microbiol 44:238–241[PubMed]
    [Google Scholar]
  15. Pakdeeto A., Tanasupawat S., Thawai C., Moonmangmee S., Kudo T., Itoh T. 2007; Salinicoccus siamensis sp. nov., isolated from fermented shrimp paste in Thailand. Int J Syst Evol Microbiol 57:2004–2008 [View Article][PubMed]
    [Google Scholar]
  16. Schleifer K. H., Kandler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–477[PubMed]
    [Google Scholar]
  17. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [View Article][PubMed]
    [Google Scholar]
  18. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
    [Google Scholar]
  19. Tindall B. J., Sikorski J., Smibert R. M., Krieg N. R. 2007; Phenotypic characterization and the principles of comparative systematics. In Methods for General and Molecular Microbiology pp. 330–393 Edited by Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G., Schmidt T. M., Snyder L. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  20. Ventosa A., Marquez M. C., Ruiz-Berraquero F, Kocur M. 1990; Salinicoccus roseus gen. nov. sp. nov., a new moderately halophilic gram-positive coccus. Syst Appl Microbiol 13:29–33 [CrossRef]
    [Google Scholar]
  21. Ventosa A., Marquez M. C., Weiss N., Tindall B. J. 1992; Transfer of Marinococcus hispanicus to the genus Salinicoccus as Salinicoccus hispanicus comb. nov.. Syst Appl Microbiol 15:530–534 [CrossRef]
    [Google Scholar]
  22. Wang X., Xue Y., Yuan S., Zhou C., Ma Y. 2008; Salinicoccus halodurans sp. nov., a moderate halophile from saline soil in China. Int J Syst Evol Microbiol 58:1537–1541 [View Article][PubMed]
    [Google Scholar]
  23. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [View Article]
    [Google Scholar]
  24. Zhang W., Xue Y., Ma Y., Zhou P., Ventosa A., Grant W. D. 2002; Salinicoccus alkaliphilus sp. nov., a novel alkaliphile and moderate halophile from Baer Soda Lake in Inner Mongolia Autonomous Region, China. Int J Syst Evol Microbiol 52:789–793 [View Article][PubMed]
    [Google Scholar]
  25. Zhang Y.-Q., Yu L.-Y., Liu H.-Y., Zhang Y.-Q., Xu L.-H., Li W.-J. 2007; Salinicoccus luteus sp. nov., isolated from a desert soil. Int J Syst Evol Microbiol 57:1901–1905 [View Article][PubMed]
    [Google Scholar]
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