1887

Abstract

Three yellow-pigmented, Gram-negative, rod-shaped, non-spore-forming bacterial strains, C36, C37 and C39, were isolated in the Medical Clinic for Small Animals and Ungulates at the University for Veterinary Medicine in Vienna, Austria. On the basis of 16S rRNA gene sequence similarity, strain C36 was shown to belong to the genus ; DSM 1045 was the nearest relative (99·5 % sequence similarity). Other species shared <97 % sequence similarity with strain C36. The presence of Q-9 as the major ubiquinone, the predominance of putrescine and spermidine in its polyamine patterns and its fatty acid profile [i.e. the predominance of C, summed feature 3 (C 7 and/or 2-OH C iso), C 7 and the presence of 3-OH C, 3-OH C and 2-OH C] were in agreement with identification of this strain as a member of the genus . Physiological and biochemical characteristics and the results of genomic fingerprinting clearly differentiated strain C36 from its phylogenetic relative DSM 1045. Results from DNA–DNA hybridization showed that strain C36 represents a species that is distinct from DSM 1045. These data demonstrate that strain C36 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is C36 (=LMG 21977=DSM 15758). Additionally, physiological, biochemical, chemotaxonomic and genomic fingerprints indicate that ATCC 29347 may not be a member of the species .

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.03024-0
2004-09-01
2019-08-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/54/5/ijs541633.html?itemId=/content/journal/ijsem/10.1099/ijs.0.03024-0&mimeType=html&fmt=ahah

References

  1. Altenburger, P., Kämpfer, P., Makristathis, A., Lubitz, W. & Busse, H.-J. ( 1996; ). Classification of bacteria isolated from a medieval wall painting. J Biotechnol 47, 39–52.[CrossRef]
    [Google Scholar]
  2. Auling, G., Busse, H.-J., Pilz, F., Webb, L., Kneifel, H. & Claus, D. ( 1991; ). Rapid differentiation, by polyamine analysis, of Xanthomonas strains from phytopathogenic pseudomonads and other members of the class Proteobacteria interacting with plants. Int J Syst Bacteriol 41, 223–228.[CrossRef]
    [Google Scholar]
  3. Busse, H.-J. & Auling, G. ( 1988; ). Polyamine pattern as a chemotaxonomic marker within the Proteobacteria. Syst Appl Microbiol 11, 1–8.[CrossRef]
    [Google Scholar]
  4. Busse, H.-J., Bunka, S., Hensel, A. & Lubitz, W. ( 1997; ). Discrimination of members of the family Pasteurellaceae based on polyamine patterns. Int J Syst Bacteriol 47, 698–708.[CrossRef]
    [Google Scholar]
  5. Busse, H.-J., Kämpfer, P. & Denner, E. B. M. ( 1999; ). Chemotaxonomic characterisation of Sphingomonas. J Ind Microbiol Biotechnol 23, 242–251.[CrossRef]
    [Google Scholar]
  6. Felsenstein, F. ( 1995; ). phylip (Phylogeny Inference Package) version 3.57c. Seattle: University of Washington.
  7. Kämpfer, P., Steiof, M. & Dott, W. ( 1991; ). Microbiological characterisation of a fuel-oil contaminated site including numerical identification of heterotrophic water and soil bacteria. Microb Ecol 21, 227–251.[CrossRef]
    [Google Scholar]
  8. Kämpfer, P., Denner, E. B. M., Meyer, S., Moore, E. R. B. & Busse, H.-J. ( 1997; ). Classification of “Pseudomonas azotocolligans” Anderson 1955, 132, in the genus Sphingomonas as Sphingomonas trueperi sp. nov. Int J Syst Bacteriol 47, 577–583.[CrossRef]
    [Google Scholar]
  9. Kämpfer, P., Buczolits, S., Albrecht, A., Busse, H.-J. & Stackebrandt, E. ( 2003; ). Towards a standardized format for the description of a novel species (of an established genus): Ochrobactrum gallinifaecis sp. nov. Int J Syst Evol Microbiol 53, 893–896.[CrossRef]
    [Google Scholar]
  10. Moore, E. R. B., Mau, M., Arnscheidt, A., Böttger, E. C., Hutson, R. A., Collins, M. D., Van De Peer, Y., De Wachter, R. & Timmis, K. N. ( 1996; ). The determination and comparison of the 16S rRNA gene sequences of species of the genus Pseudomonas (sensu stricto) and estimation of the natural intrageneric relationships. Syst Appl Microbiol 19, 478–492.[CrossRef]
    [Google Scholar]
  11. Oyaizu, H. & Komagata, K. ( 1983; ). Grouping of Pseudomonas species on the basis of cellular fatty acid composition and the quinone system with special reference to the existence of 3-hydroxy fatty acids. J Gen Appl Microbiol 29, 17–40.[CrossRef]
    [Google Scholar]
  12. Page, R. D. M. ( 1996; ). treeview: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12, 357–358.
    [Google Scholar]
  13. Pearson, W. R. & Lipman, D. J. ( 1988; ). Improved tools for biological sequence comparison. Proc Natl Acad Sci U S A 85, 2444–2448.[CrossRef]
    [Google Scholar]
  14. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  15. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  16. Tindall, B. J. ( 1990; ). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13, 128–130.[CrossRef]
    [Google Scholar]
  17. van Beilen, J. B., Panke, S., Lucchini, S., Franchini, A. G., Röthlisberger, M. & Witholt, B. ( 2001; ). Analysis of Pseudomonas putida alkane-degradation gene clusters and flanking insertion sequences: evolution and regulation of the alk genes. Microbiology 147, 1621–1630.
    [Google Scholar]
  18. Wieser, M. & Busse, H.-J. ( 2000; ). Rapid identification of Staphylococcus epidermidis. Int J Syst Evol Microbiol 50, 1087–1093.[CrossRef]
    [Google Scholar]
  19. Ziemke, F., Höfle, M. G., Lalucat, J. & Rosselló-Mora, R. ( 1998; ). Reclassification of Shewanella putrefaciens Owen's genomic group II as Shewanella baltica sp. nov. Int J Syst Bacteriol 48, 179–186.[CrossRef]
    [Google Scholar]
  20. Zlamala, C., Schumann, P., Kämpfer, P., Rosselló-Mora, R., Lubitz, W. & Busse, H.-J. ( 2002; ). Agrococcus baldri sp. nov., isolated from the air in the ‘Virgilkapelle’ in Vienna. Int J Syst Evol Microbiol 52, 1211–1216.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.03024-0
Loading
/content/journal/ijsem/10.1099/ijs.0.03024-0
Loading

Data & Media loading...

vol. , part 5, pp. 1633 – 1637

Relative cellular fatty acid composition of C36 , C37, C39, DSM 1045 and ATCC 29347

Two-dimensional TLC of polar lipids of strains C36 , DSM 1045 and ATCC 29347

SDS-PAGE of strains C36 , C37, C39, DSM 1045 and ATCC 29347

[Single PDF file](185 KB)



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error