1887

Abstract

A Gram-negative-staining, rod-shaped, non-motile and pink bacterial strain was isolated from the soil of a forest located in Xinjiang, China. The strain, designated strain HYL7-15, was subjected to a taxonomic analysis using a polyphasic approach. 16S rRNA gene sequence analyses indicated that the isolate belonged to the phylum and was related to the genus , with sequence similarities ranging from 93.1 to 95.0 % with other species of the genus . Strain HYL7-15 contained MK-7 as the predominant menaquinone and its DNA G+C content was 44.9 mol%. The major cellular fatty acids of the novel strain were iso-C (16.49 %), iso-C 3-OH (10.96 %) and summed feature 4 (comprising anteiso-C B and/or iso-C I, 18.46 %). The major polar lipids were phosphatidylethanolamine (PE) and phosphatidylglycerol (PG); diphosphatidylglycerol (DPG), three unknown aminophospholipids (APLs) and two unknown phospholipids (PLs) were also detected. On the basis of the evidence presented, it is concluded that strain HYL7-15 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is HYL7-15 ( = CCTCC AB 206239 = NRRL B-59488).

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2012-03-01
2019-12-15
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References

  1. Bowman J. P. . ( 2000; ). Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov.. Int J Syst Evol Microbiol 50:, 1861–1868.[PubMed]
    [Google Scholar]
  2. Chun J. , Lee J.-H. , Jung Y. , Kim M. , Kim S. , Kim B. K. , Lim Y. W. . ( 2007; ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. . Int J Syst Evol Microbiol 57:, 2259–2261. [CrossRef] [PubMed]
    [Google Scholar]
  3. Dastager S. G. , Raziuddin Q. S. , Deepa C. K. , Li W.-J. , Pandey A. . ( 2010; ). Pontibacter niistensis sp. nov., isolated from forest soil in India. . Int J Syst Evol Microbiol 60:, 2867–2870. [CrossRef]
    [Google Scholar]
  4. Doetsch R. N. . ( 1981; ). Determinative methods of light microscopy. . In Manual of Methods for General Bacteriology, pp. 21–33. Edited by Gerhardt P. , Murray R. G. E. , Costilow R. N. , Nester E. W. , Wood W. A. , Krieg N. R. , Phillips G. H. . . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  5. Felsenstein J. . ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  6. Fitch W. M. . ( 1971; ). Towards defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20:, 406–416. [CrossRef]
    [Google Scholar]
  7. Kimura M. . ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef] [PubMed]
    [Google Scholar]
  8. Kovács N. . ( 1956; ). Identification of Pseudomonas pyocyanea by the oxidase reaction. . Nature 178:, 703–704. [CrossRef] [PubMed]
    [Google Scholar]
  9. Kuykendall L. D. , Roy M. A. , O’Neill J. J. , Devine T. E. . ( 1988; ). Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum . . Int J Syst Bacteriol 38:, 358–361. [CrossRef]
    [Google Scholar]
  10. Lane D. J. . ( 1991; ). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by Stackebrandt E. , Goodfellow M. . . Chichester:: Wiley;.
    [Google Scholar]
  11. Mesbah M. , Premachandran U. , Whitman W. B. . ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  12. Nedashkovskaya O. I. , Kim S. B. , Suzuki M. , Shevchenko L. S. , Lee M. S. , Lee K. H. , Park M. S. , Frolova G. M. , Oh H. W. . & other authors ( 2005; ). Pontibacter actiniarum gen. nov., sp. nov., a novel member of the phylum ‘Bacteroidetes’, and proposal of Reichenbachiella gen. nov. as a replacement for the illegitimate prokaryotic generic name Reichenbachia Nedashkovskaya et al. 2003. . Int J Syst Evol Microbiol 55:, 2583–2588. [CrossRef] [PubMed]
    [Google Scholar]
  13. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  14. Sambrook J. , Fritsch E. F. , Maniatis T. . ( 1989; ). Molecular Cloning: a Laboratory Manual, , 2nd edn.. Cold Spring Harbor, NY:: Cold Spring Harbor Laboratory;.
    [Google Scholar]
  15. Sasser M. . ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc.;
  16. Smibert R. M. , Krieg N. R. . ( 1994; ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P. , Murray R. G. E. , Wood W. A. , Krieg N. R. . . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  17. Stackebrandt E. , Goebel B. M. . ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44:, 846–849. [CrossRef]
    [Google Scholar]
  18. Suresh K. , Mayilraj S. , Chakrabarti T. . ( 2006; ). Effluviibacter roseus gen. nov., sp. nov., isolated from muddy water, belonging to the family “Flexibacteraceae” . . Int J Syst Evol Microbiol 56:, 1703–1707. [CrossRef] [PubMed]
    [Google Scholar]
  19. Tamura K. , Dudley J. , Nei M. , Kumar S. . ( 2007; ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef] [PubMed]
    [Google Scholar]
  20. Tindall B. J. . ( 1990; ). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. . Syst Appl Microbiol 13:, 128–130.[CrossRef]
    [Google Scholar]
  21. Ventosa A. , Marquez M. C. , Kocur M. , Tindall B. J. . ( 1993; ). Comparative study of “Micrococcus sp.” strains CCM 168 and CCM 1405 and members of the genus Salinicoccus . . Int J Syst Bacteriol 43:, 245–248. [CrossRef] [PubMed]
    [Google Scholar]
  22. Wang Y. , Zhang K. , Cai F. , Zhang L. , Tang Y. , Dai J. , Fang C.-X. . ( 2010; ). Pontibacter xinjiangensis sp. nov., in the phylum ‘Bacteroidetes’, and reclassification of [Effluviibacter] roseus as Pontibacter roseus comb. nov.. Int J Syst Evol Microbiol 60:, 99–103. [CrossRef] [PubMed]
    [Google Scholar]
  23. Xie C. H. , Yokota A. . ( 2003; ). Phylogenetic analyses of Lampropedia hyalina based on the 16S rRNA gene sequence. . J Gen Appl Microbiol 49:, 345–349. [CrossRef] [PubMed]
    [Google Scholar]
  24. Zhang L. , Zhang Q. J. , Luo X. S. , Tang Y. L. , Dai J. , Li Y. W. , Wang Y. , Chen G. , Fang C. X. . ( 2008; ). Pontibacter korlensis sp. nov., isolated from the desert of Xinjiang, China. . Int J Syst Evol Microbiol 58:, 1210–1214. [CrossRef] [PubMed]
    [Google Scholar]
  25. Zhou Y. , Wang X. , Liu H. , Zhang K. Y. , Zhang Y. Q. , Lai R. , Li W. J. . ( 2007; ). Pontibacter akesuensis sp. nov., isolated from a desert soil in China. . Int J Syst Evol Microbiol 57:, 321–325. [CrossRef] [PubMed]
    [Google Scholar]
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