1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 62, Issue 1

sp. nov., a filamentous actinomycete isolated from a hypersaline habitat Free

Abstract

A novel actinomycete strain, designated TRM F103, was isolated from a hypersaline habitat of the Tarim basin in Xinjiang province, north-west China. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate belonged to the genus and was most closely related to YIM 93223 (99.3 % 16S rRNA gene sequence similarity). However, DNA–DNA relatedness between these two strains, based on triplicate experiments, was only 31.6 %. The isolate contained -diaminopimelic acid and ribose, glucose and galactose as the major whole-cell sugars. The predominant menaquinone was MK-8(H). The major fatty acids were iso-C and C. The polar lipids were diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine and glucosamine-containing phospholipids. The G+C content of the genomic DNA was 66.4 mol%. The phenotypic data clearly distinguished the isolate from its closest relatives. The combined phylogenetic, chemotaxonomic and phenotypic data indicate that the isolate represents a novel species of the genus . The proposed name is sp. nov., with TRM F103 ( = JCM 15899 = CCTCC AB 208326) as the type strain.

  • Published Online:
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31060001 and 31060003)
  • Key Project for Department of Science and Technology of Ministry of Education of China (Award 209145)
  • 973 Program (Award 2010CB134505)
  • Opening Project by State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences (Award SKLMR-20090603)
  • Opening Project of Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography
© 2012 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.030031-0
2012-01-01
2024-12-02
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/1/23.html?itemId=/content/journal/ijsem/10.1099/ijs.0.030031-0&mimeType=html&fmt=ahah

References

  1. Bian J., Li Y., Wang J., Song F.-H., Liu M., Dai H.-Q., Ren B., Gao H., Hu X. et al. other authors 2009; Amycolatopsis marina sp. nov., an actinomycete isolated from an ocean sediment. Int J Syst Evol Microbiol 59:477–481 [View Article][PubMed]
     [Google Scholar]
  2. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y.-W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [View Article][PubMed]
     [Google Scholar]
  3. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. 1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230[PubMed] [CrossRef]
     [Google Scholar]
  4. Cui X.-L., Mao P.-H., Zeng M., Li W.-J., Zhang L.-P., Xu L.-H., Jiang C.-L. 2001; Streptimonospora salina gen. nov., sp. nov., a new member of the family Nocardiopsaceae . Int J Syst Evol Microbiol 51:357–363[PubMed]
     [Google Scholar]
  5. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–789 [View Article]
     [Google Scholar]
  6. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [View Article]
     [Google Scholar]
  7. Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. (editors) 1994 Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology;
     [Google Scholar]
  8. Groth I., Schumann P., Rainey F. A., Martin K., Schuetze B., Augsten K. 1997; Demetria terragena gen. nov., sp. nov., a new genus of actinomycetes isolated from compost soil. Int J Syst Bacteriol 47:1129–1133 [View Article][PubMed]
     [Google Scholar]
  9. Guan T.-W., Xiao J., Zhao K., Luo X.-X., Zhang X.-P., Zhang L.-L. 2010; Halomonas xinjiangensis sp. nov., a halotolerant bacterium isolated from a salt lake. Int J Syst Evol Microbiol 60:349–352 [View Article][PubMed]
     [Google Scholar]
  10. Hasegawa T., Takizawa M., Tanida S. 1983; A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29:319–322 [View Article]
     [Google Scholar]
  11. He L., Li W., Huang Y., Wang L., Liu Z., Lanoot B., Vancanneyt M., Swings J. 2005; Streptomyces jietaisiensis sp. nov., isolated from soil in northern China. Int J Syst Evol Microbiol 55:1939–1944 [View Article][PubMed]
     [Google Scholar]
  12. Huang Y., Paściak M., Liu Z., Xie Q., Gamian A. 2004; Amycolatopsis palatopharyngis sp. nov., a potentially pathogenic actinomycete isolated from a human clinical source. Int J Syst Evol Microbiol 54:359–363 [View Article][PubMed]
     [Google Scholar]
  13. Kelly K. L. 1964 Inter-Society Color Council – National Bureau of Standards Color Name Charts Illustrated with Centroid Colors Washington, DC: US Government Printing Office;
     [Google Scholar]
  14. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [View Article][PubMed]
     [Google Scholar]
  15. Lechevalier M. P., Prauser H., Labeda D. P., Ruan J.-S. 1986; Two new genera of nocardioform actinomycetes: Amycolata gen. nov. and Amycolatopsis gen. nov.. Int J Syst Bacteriol 36:29–37 [View Article]
     [Google Scholar]
  16. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement. of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
     [Google Scholar]
  17. Minnikin D. E., Hutchinson I. G., Caldicott A. B., Goodfellow M. 1980; Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatogr A 188:221–233 [View Article]
     [Google Scholar]
  18. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [View Article]
     [Google Scholar]
  19. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
     [Google Scholar]
  20. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc;
  21. Shirling E. B., Gottlieb D. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340 [View Article]
     [Google Scholar]
  22. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp. 607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
     [Google Scholar]
  23. Stackebrandt E., Rainey F. A., Ward-Rainey N. L. 1997; Proposal for a new hierarchic classification system, Actinobacteria classis nov.. Int J Syst Bacteriol 47:479–491 [View Article]
     [Google Scholar]
  24. Staneck J. L., Roberts G. D. 1974; Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28:226–231[PubMed]
     [Google Scholar]
  25. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: Molecular Evolutionary Genetics Analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [View Article][PubMed]
     [Google Scholar]
  26. Tang S.-K., Wang Y., Guan T.-W., Lee J.-C., Kim C.-J., Li W.-J. 2010; Amycolatopsis halophila sp. nov., a halophilic actinomycete isolated from a salt lake. Int J Syst Evol Microbiol 60:1073–1078 [View Article][PubMed]
     [Google Scholar]
  27. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. et al. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [View Article]
     [Google Scholar]
  28. Williams S. T. 1967; Sensitivity of streptomycetes to antibiotics as a taxonomic character. J Gen Microbiol 46:151–160[PubMed] [CrossRef]
     [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.030031-0
Loading
Amycolatopsis salitolerans sp. nov., a filamentous actinomycete isolated from a hypersaline habitat
Int J Syst Evol Microbiol 62, 23 (2012); https://doi.org/10.1099/ijs.0.030031-0
/content/journal/ijsem/10.1099/ijs.0.030031-0
/content/journal/ijsem/10.1099/ijs.0.030031-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error