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Abstract

A novel actinomycete strain, designated TRM F103, was isolated from a hypersaline habitat of the Tarim basin in Xinjiang province, north-west China. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate belonged to the genus and was most closely related to YIM 93223 (99.3 % 16S rRNA gene sequence similarity). However, DNA–DNA relatedness between these two strains, based on triplicate experiments, was only 31.6 %. The isolate contained -diaminopimelic acid and ribose, glucose and galactose as the major whole-cell sugars. The predominant menaquinone was MK-8(H). The major fatty acids were iso-C and C. The polar lipids were diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine and glucosamine-containing phospholipids. The G+C content of the genomic DNA was 66.4 mol%. The phenotypic data clearly distinguished the isolate from its closest relatives. The combined phylogenetic, chemotaxonomic and phenotypic data indicate that the isolate represents a novel species of the genus . The proposed name is sp. nov., with TRM F103 ( = JCM 15899 = CCTCC AB 208326) as the type strain.

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31060001 and 31060003)
  • Key Project for Department of Science and Technology of Ministry of Education of China (Award 209145)
  • 973 Program (Award 2010CB134505)
  • Opening Project by State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences (Award SKLMR-20090603)
  • Opening Project of Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography
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2012-01-01
2021-10-18
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