1887

Abstract

The taxonomic position of a streptomycete isolated from soil collected from Cockle Park Experimental Farm, Northumberland, UK, was determined by using a polyphasic approach. The organism had chemical and morphological features consistent with its classification in the genus 16S rRNA gene sequence analysis supported classification of the strain in the genus and showed that it formed a distinct phyletic line loosely associated with members of the clade. It was related most closely to 1413 (98.6 %16S rRNA gene sequence similarity), but could be distinguished from the latter based on the low level of DNA–DNA relatedness (40 %). It was readily distinguished from the type strains of all species assigned to the clade based on a combination of phenotypic properties. Strain BK168 ( = KACC 20908 = NCIMB 14704) should therefore be classified as the type strain of a novel species of the genus , for which the name sp. nov. is proposed. The organism produces the antibiotic dioxamycin.

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2012-02-01
2019-12-08
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References

  1. Anderson A. S. , Wellington E. M. H. . ( 2001; ). The taxonomy of Streptomyces and related genera. . Int J Syst Evol Microbiol 51:, 797–814. [CrossRef] [PubMed]
    [Google Scholar]
  2. Atalan E. , Manfio G. P. , Ward A. C. , Kroppenstedt R. M. , Goodfellow M. . ( 2000; ). Biosystematic studies on novel streptomycetes from soil. . Antonie van Leeuwenhoek 77:, 337–353. [CrossRef] [PubMed]
    [Google Scholar]
  3. Bérdy J. . ( 2005; ). Bioactive microbial metabolites. . J Antibiot (Tokyo) 58:, 1–26. [CrossRef] [PubMed]
    [Google Scholar]
  4. Collins M. D. . ( 1985; ). Isoprenoid quinone analysis in classification and identification. . In Chemical Methods in Bacterial Systematics, pp. 267–287. Edited by Goodfellow M. , Minnikin D. E. . . London:: Academic Press;.
    [Google Scholar]
  5. Felsenstein J. . ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef] [PubMed]
    [Google Scholar]
  6. Felsenstein J. . ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  7. Fiedler H.-P. . ( 1993; ). Biosynthetic capacities of actinomycetes. 1. Screening for secondary metabolites by HPLC and UV-visible absorbance spectral libraries. . Nat Prod Lett 2:, 119–128. [CrossRef]
    [Google Scholar]
  8. Fitch W. M. . ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20:, 406–416. [CrossRef]
    [Google Scholar]
  9. Gonzalez J. M. , Saiz-Jimenez C. . ( 2002; ). A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. . Environ Microbiol 4:, 770–773. [CrossRef]
    [Google Scholar]
  10. Goodfellow M. , Fiedler H.-P. . ( 2010; ). A guide to successful bioprospecting: informed by actinobacterial systematics. . Antonie van Leeuwenhoek 98:, 119–142. [CrossRef] [PubMed]
    [Google Scholar]
  11. Goodfellow M. , Kumar Y. , Labeda D. P. , Sembiring L. . ( 2007; ). The Streptomyces violaceusniger clade: a home for streptomycetes with rugose ornamented spores. . Antonie van Leeuwenhoek 92:, 173–199. [CrossRef] [PubMed]
    [Google Scholar]
  12. Guindon S. , Gascuel O. . ( 2003; ). A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. . Syst Biol 52:, 696–704. [CrossRef] [PubMed]
    [Google Scholar]
  13. Hasegawa T. , Takizawa M. , Tanida S. . ( 1983; ). A rapid analysis for chemical grouping of aerobic actinomycetes. . J Gen Appl Microbiol 29:, 319–322. [CrossRef]
    [Google Scholar]
  14. Jones K. L. . ( 1949; ). Fresh isolates of actinomycetes in which the presence of sporogenous aerial mycelia is a fluctuating characteristic. . J Bacteriol 57:, 141–145.
    [Google Scholar]
  15. Jukes T. H. , Cantor C. R. . ( 1969; ). Evolution of protein molecules. . In Mammalian Protein Metabolism, vol. 3, pp. 21–123. Edited by Munro H. N. . . New York:: Academic Press;.
    [Google Scholar]
  16. Kim D. , Chun J. , Sahin N. , Hah Y. C. , Goodfellow M. . ( 1996; ). Analysis of thermophilic clades within the genus Streptomyces by 16S rRNA ribosomal DNA sequence comparisons. . Int J Syst Bacteriol 46:, 581–587. [CrossRef]
    [Google Scholar]
  17. Kim S. B. , Seong C. N. , Jeon S. J. , Bae K. S. , Goodfellow M. . ( 2004; ). Taxonomic study of neutrotolerant acidophilic actinomycetes isolated from soil and description of Streptomyces yeochonensis sp. nov.. Int J Syst Evol Microbiol 54:, 211–214. [CrossRef] [PubMed]
    [Google Scholar]
  18. Kumar Y. , Goodfellow M. . ( 2010; ). Reclassification of Streptomyces hygroscopicus strains as Streptomyces aldersoniae sp. nov., Streptomyces angustmyceticus sp. nov., comb. nov., Streptomyces ascomycinicus sp. nov., Streptomyces decoyicus sp. nov., comb. nov., Streptomyces milbemycinicus sp. nov. and Streptomyces wellingtoniae sp. nov.. Int J Syst Evol Microbiol 60:, 769–775. [CrossRef] [PubMed]
    [Google Scholar]
  19. Küster E. , Williams S. T. . ( 1964; ). Selective media for isolation of streptomycetes. . Nature 202:, 928–929. [CrossRef] [PubMed]
    [Google Scholar]
  20. Lanoot B. , Vancanneyt M. , Dawyndt P. , Cnockaert M. , Zhang J. , Huang Y. , Liu Z. , Swings J. . ( 2004; ). BOX-PCR fingerprinting as a powerful tool to reveal synonymous names in the genus Streptomyces. Emended descriptions are proposed for the species Streptomyces cinereorectus, S. fradiae, S. tricolor, S. colombiensis, S. filamentosus, S. vinaceus and S. phaeopurpureus . . Syst Appl Microbiol 27:, 84–92. [CrossRef] [PubMed]
    [Google Scholar]
  21. Lanoot B. , Vancanneyt M. , Hoste B. , Vandemeulebroecke K. , Cnockaert M. C. , Dawyndt P. , Liu Z. , Huang Y. , Swings J. . ( 2005a; ). Grouping of streptomycetes using 16S-ITS RFLP fingerprinting. . Res Microbiol 156:, 755–762. [CrossRef] [PubMed]
    [Google Scholar]
  22. Lanoot B. , Vancanneyt M. , Van Schoor A. , Liu Z. , Swings J. . ( 2005b; ). Reclassification of Streptomyces nigrifaciens as a later synonym of Streptomyces flavovirens; Streptomyces citreofluorescens, Streptomyces chrysomallus subsp. chrysomallus and Streptomyces fluorescens as later synonyms of Streptomyces anulatus; Streptomyces chibaensis as a later synonym of Streptomyces corchorusii; Streptomyces flaviscleroticus as a later synonym of Streptomyces minutiscleroticus; and Streptomyces lipmanii, Streptomyces griseus subsp. alpha, Streptomyces griseus subsp. cretosus and Streptomyces willmorei as later synonyms of Streptomyces microflavus . . Int J Syst Evol Microbiol 55:, 729–731. [CrossRef] [PubMed]
    [Google Scholar]
  23. Lechevalier H. A. , Lechevalier M. P. . ( 1970; ). A critical evaluation of the genera of aerobic actinomycetes. . In The Actinomycetales, pp. 393–405. Edited by Prauser H. . . Jena:: VEB Gustav Fischer;.
    [Google Scholar]
  24. Liu Z. , Shi Y. , Zhang Y. , Zhou Z. , Lu Z. , Li W. , Huang Y. , Rodríguez C. , Goodfellow M. . ( 2005; ). Classification of Streptomyces griseus (Krainsky 1914) Waksman and Henrici 1948 and related species and the transfer of ‘Microstreptospora cinerea’ to the genus Streptomyces as Streptomyces yanii sp. nov.. Int J Syst Evol Microbiol 55:, 1605–1610. [CrossRef] [PubMed]
    [Google Scholar]
  25. Manfio G. P. , Zakrzewska-Czerwinska J. , Atalan E. , Goodfellow M. . ( 1995; ). Towards minimal standards for the description of Streptomyces species. . Biotekhnologiia 8:, 228–237.
    [Google Scholar]
  26. MIDI ( 1999; ). Sherlock Microbial Identification System, Operating Manual, version 30. . Newark, DE:, MIDI Inc;.
  27. O’Donnell A. G. , Falconer C. , Goodfellow M. , Ward A. C. , Williams E. . ( 1993; ). Biosystematics and diversity amongst novel carboxydotrophic actinomycetes. . Antonie van Leeuwenhoek 64:, 325–340. [CrossRef] [PubMed]
    [Google Scholar]
  28. Quintana E. T. , Wierzbicka K. , Mackiewicz P. , Osman A. , Fahal A. H. , Hamid M. E. , Zakrzewska-Czerwinska J. , Maldonado L. A. , Goodfellow M. . ( 2008; ). Streptomyces sudanensis sp. nov., a new pathogen isolated from patients with actinomycetoma. . Antonie van Leeuwenhoek 93:, 305–313. [CrossRef] [PubMed]
    [Google Scholar]
  29. Rong X. , Huang Y. . ( 2010; ). Taxonomic evaluation of the Streptomyces griseus clade using multilocus sequence analysis and DNA–DNA hybridization, with proposal to combine 29 species and three subspecies as 11 genomic species. . Int J Syst Evol Microbiol 60:, 696–703. [CrossRef] [PubMed]
    [Google Scholar]
  30. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  31. Sawa R. , Matsuda N. , Uchida T. , Ikeda T. , Sawa T. , Naganawa H. , Hamada M. , Takeuchi T. . ( 1991; ). Dioxamycin, a new benz[a]anthraquinone antibiotic. . J Antibiot (Tokyo) 44:, 396–402.[PubMed] [CrossRef]
    [Google Scholar]
  32. Seldin L. , Dubnau D. . ( 1985; ). Deoxyribonucleic acid homology among Bacillus polymyxa, Bacillus macerans, Bacillus azotofixans, and other nitrogen-fixing Bacillus strains. . Int J Syst Bacteriol 35:, 151–154. [CrossRef]
    [Google Scholar]
  33. Shirling E. B. , Gottlieb D. . ( 1966; ). Methods for characterization of Streptomyces species. . Int J Syst Bacteriol 16:, 313–340. [CrossRef]
    [Google Scholar]
  34. Tamura K. , Dudley J. , Nei M. , Kumar S. . ( 2007; ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef] [PubMed]
    [Google Scholar]
  35. Uchida K. , Kudo T. , Suzuki K. I. , Nakase T. . ( 1999; ). A new rapid method of glycolate test by diethyl ether extraction, which is applicable to a small amount of bacterial cells of less than one milligram. . J Gen Appl Microbiol 45:, 49–56. [CrossRef] [PubMed]
    [Google Scholar]
  36. Wayne L. G. , Brenner D. J. , Colwell R. R. , Grimont P. A. D. , Kandler O. , Krichevsky M. I. , Moore L. H. , Moore W. E. C. , Murray R. G. E. . & other authors ( 1987; ). International Committee on Bacterial Systematics. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
  37. Williams S. T. , Goodfellow M. , Alderson G. , Wellington E. M. H. , Sneath P. H. A. , Sackin M. J. . ( 1983; ). Numerical classification of Streptomyces and related genera. . J Gen Microbiol 129:, 1743–1813.[PubMed]
    [Google Scholar]
  38. Williams S. T. , Goodfellow M. , Alderson G. . ( 1989; ). Genus Streptomyces Waksman and Henrici 1943, 339AL. . In Bergey’s Manual of Systematic Bacteriology, vol. 4, pp. 2452–2492. Edited by Williams S. T. , Sharpe M. E. , Holt J. G. . . Baltimore:: Williams & Wilkins;.
    [Google Scholar]
  39. Xu C. , Wang L. , Cui Q. , Huang Y. , Liu Z. , Zheng G. , Goodfellow M. . ( 2006; ). Neutrotolerant acidophilic Streptomyces species isolated from acidic soils in China: Streptomyces guanduensis sp. nov., Streptomyces paucisporeus sp. nov., Streptomyces rubidus sp. nov. and Streptomyces yanglinensis sp. nov.. Int J Syst Evol Microbiol 56:, 1109–1115. [CrossRef] [PubMed]
    [Google Scholar]
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