1887

Abstract

A novel coccoid-shaped strain, AS/ASP6 (II), was isolated from a sample taken from (Schulze), a marine sponge, collected at a depth of 30 m from the Bay of Bengal. This strain was identified by using a polyphasic taxonomic approach. The results of 16S rRNA gene sequence analysis showed that strain AS/ASP6 (II) should be assigned to the genus Chemotaxonomic data (A4α-type peptidoglycan; MK-6, MK-7 and MK-8 menaquinones; mainly branched cellular fatty acids; and phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol as cellular phospholipids) supported taxonomic placement in the genus . Phylogenetic analysis of the 16S rRNA gene sequence showed that strain AS/ASP6 (II) belonged to the genus and was closely related to the type strains of (99.1 %) followed by (98.6 %), (98.5 %), (98.3 %), (98.1 %), (97.9 %), (97.8 %) and (97.7 %); DNA–DNA hybridization values obtained were well below the threshold that is required for the proposal of a novel species. The G+C content of the genomic DNA was 51.0 mol%. The phenotypic and genotypic data showed that strain AS/ASP6 (II) merits recognition as a representative of a novel species of the genus , for which the name sp. nov. is proposed; the type strain is AS/ASP6 (II) ( = MTCC 8491 = DSM 23997).

Funding
This study was supported by the:
  • CSIR, Government of India (Award NWP-006)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.029967-0
2012-04-01
2024-12-03
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/4/883.html?itemId=/content/journal/ijsem/10.1099/ijs.0.029967-0&mimeType=html&fmt=ahah

References

  1. Alam S. I., Singh L., Dube S., Reddy G. S. N., Shivaji S. 2003; Psychrophilic Planococcus maitriensis sp. nov. from Antarctica. Syst Appl Microbiol 26:505–510 [View Article][PubMed]
    [Google Scholar]
  2. Choi J.-H., Im W.-T., Liu Q.-M., Yoo J.-S., Shin J.-H., Rhee S.-K., Roh D.-H. 2007; Planococcus donghaensis sp. nov., a starch-degrading bacterium isolated from the East Sea, South Korea. Int J Syst Evol Microbiol 57:2645–2650 [View Article][PubMed]
    [Google Scholar]
  3. Chun J., Lee J. H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [View Article][PubMed]
    [Google Scholar]
  4. Cowan S. T., Steel K. J. 1965 Manual for the Identification of Medical Bacteria London: Cambridge University Press;
    [Google Scholar]
  5. Dai X., Wang Y. N., Wang B. J., Liu S. J., Zhou Y. G. 2005; Planomicrobium chinense sp. nov., isolated from coastal sediment, and transfer of Planococcus psychrophilus and Planococcus alkanoclasticus to Planomicrobium as Planomicrobium psychrophilum comb. nov. and Planomicrobium alkanoclasticum comb. nov.. Int J Syst Evol Microbiol 55:699–702 [View Article][PubMed]
    [Google Scholar]
  6. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
    [Google Scholar]
  7. Hao M. V., Komagata K. 1985; A new species of Planococcus, P. kocurii isolated from fish, frozen foods, and fish curing brine. J Gen Appl Microbiol 31:441–455 [View Article]
    [Google Scholar]
  8. Jung Y.-T., Kang S.-J., Oh T.-K., Yoon J. H., Kim B.-H. 2009; Planomicrobium flavidum sp. nov., isolated from a marine solar saltern, and transfer of Planococcus stackebrandtii Mayilraj et al. 2005 to the genus Planomicrobium as Planomicrobium stackebrandtii comb. nov.. Int J Syst Evol Microbiol 59:2929–2933 [View Article][PubMed]
    [Google Scholar]
  9. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [View Article][PubMed]
    [Google Scholar]
  10. Kroppenstedt R. M. 1982; Separation of bacterial menaquinones by HPLC using reverse phase (RP 18) and a silver loaded ion exchanger. J Liq Chromatogr 5:2359–2367 [View Article]
    [Google Scholar]
  11. Mayilraj S., Prasad G. S., Suresh K., Saini H. S., Shivaji S., Chakrabarti T. 2005; Planococcus stackebrandtii sp. nov., isolated from a cold desert of the Himalayas, India. Int J Syst Evol Microbiol 55:91–94 [View Article][PubMed]
    [Google Scholar]
  12. Mayilraj S., Kroppenstedt R. M., Suresh K., Saini H. S. 2006; Kocuria himachalensis sp. nov., an actinobacterium isolated from the Indian Himalayas. Int J Syst Evol Microbiol 56:1971–1975 [View Article][PubMed]
    [Google Scholar]
  13. Migula W. 1894; Über ein neues System der Bakterien. Arb Bakteriol Inst Karlsruhe 1:235–238
    [Google Scholar]
  14. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [View Article]
    [Google Scholar]
  15. Nakagawa Y., Sakane T., Yokota A. 1996; Emendation of the genus Planococcus and transfer of Flavobacterium okeanokoites Zobell and Upham 1944 to the genus Planococcus as Planococcus okeanokoites comb. nov.. Int J Syst Bacteriol 46:866–870 [View Article][PubMed]
    [Google Scholar]
  16. Pandey K. K., Mayilraj S., Chakrabarti T. 2002; Pseudomonas indica sp. nov., a novel butane-utilizing species. Int J Syst Evol Microbiol 52:1559–1567 [View Article][PubMed]
    [Google Scholar]
  17. Perrière G., Gouy M. 1996; WWW-query: an on-line retrieval system for biological sequence banks. Biochimie 78:364–369 [View Article][PubMed]
    [Google Scholar]
  18. Reddy G. S. N., Prakash J. S. S., Vairamani M., Prabhakar S., Matsumoto G. I., Shivaji S. 2002; Planococcus antarcticus and Planococcus psychrophilus spp. nov. isolated from cyanobacterial mat samples collected from ponds in Antarctica. Extremophiles 6:253–261 [View Article][PubMed]
    [Google Scholar]
  19. Romano I., Giordano A., Lama L., Nicolaus B., Gambacorta A. 2003; Planococcus rifietensis sp. nov., isolated from algal mat collected from a sulfurous spring in Campania (Italy). Syst Appl Microbiol 26:357–366 [View Article][PubMed]
    [Google Scholar]
  20. Saha P., Krishnamurthi S., Mayilraj S., Prasad G. S., Bora T. C., Chakrabarti T. 2005; Aquimonas voraii gen. nov., sp. nov., a novel gammaproteobacterium isolated from a warm spring of Assam, India. Int J Syst Evol Microbiol 55:1491–1495 [View Article][PubMed]
    [Google Scholar]
  21. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  22. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc;
  23. Schleifer K. H. 1985; Analysis of the chemical composition and primary structure of murein. Methods Microbiol 18:123–156 [View Article]
    [Google Scholar]
  24. Schleifer K. H., Kandler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–477[PubMed]
    [Google Scholar]
  25. Skerman V. B. D. 1967 A Guide to the Identification of the Genera of Bacteria, 2nd edn. Baltimore: Williams & Wilkins;
    [Google Scholar]
  26. Smith N. R., Gordon R. E., Clark F. E. 1952; Aerobic spore-forming bacteria, USDA Agriculture Monograph no. 16. Washington DC: Government Printing Office;
  27. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [View Article]
    [Google Scholar]
  28. Suresh K., Mayilraj S., Bhattacharya A., Chakrabarti T. 2007; Planococcus columbae sp. nov., isolated from pigeon faeces. Int J Syst Evol Microbiol 57:1266–1271 [View Article][PubMed]
    [Google Scholar]
  29. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
    [Google Scholar]
  30. Tindall B. J. 1990a; A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13:128–130 [View Article]
    [Google Scholar]
  31. Tindall B. J. 1990b; Lipid composition of Halobacterium lacusprofundi . FEMS Microbiol Lett 66:199–202 [View Article]
    [Google Scholar]
  32. Tourova T. P., Antonov A. S. 1988; Identification of microorganisms by rapid DNA–DNA hybridization. Methods Microbiol 19:333–355 [View Article]
    [Google Scholar]
  33. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [View Article]
    [Google Scholar]
  34. Xu P., Li W. J., Tang S. K., Zhang Y. Q., Chen G. Z., Chen H. H., Xu L. H., Jiang C. L. 2005; Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family ‘Oxalobacteraceae’ isolated from China. Int J Syst Evol Microbiol 55:1149–1153 [View Article][PubMed]
    [Google Scholar]
  35. Yoon J.-H., Weiss N., Kang K. H., Oh T.-K., Park Y.-H. 2003; Planococcus maritimus sp. nov., isolated from sea water of a tidal flat in Korea. Int J Syst Evol Microbiol 53:2013–2017 [View Article][PubMed]
    [Google Scholar]
  36. Yoon J.-H., Kang S.-J., Lee S.-Y., Oh K.-H., Oh T.-K. 2010; Planococcus salinarum sp. nov., isolated from a marine solar saltern, and emended description of the genus Planococcus. . Int J Syst Evol Microbiol 60:754–758 [View Article][PubMed]
    [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.029967-0
Loading
/content/journal/ijsem/10.1099/ijs.0.029967-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error