1887

Abstract

A novel coccoid-shaped strain, AS/ASP6 (II), was isolated from a sample taken from (Schulze), a marine sponge, collected at a depth of 30 m from the Bay of Bengal. This strain was identified by using a polyphasic taxonomic approach. The results of 16S rRNA gene sequence analysis showed that strain AS/ASP6 (II) should be assigned to the genus Chemotaxonomic data (A4α-type peptidoglycan; MK-6, MK-7 and MK-8 menaquinones; mainly branched cellular fatty acids; and phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol as cellular phospholipids) supported taxonomic placement in the genus . Phylogenetic analysis of the 16S rRNA gene sequence showed that strain AS/ASP6 (II) belonged to the genus and was closely related to the type strains of (99.1 %) followed by (98.6 %), (98.5 %), (98.3 %), (98.1 %), (97.9 %), (97.8 %) and (97.7 %); DNA–DNA hybridization values obtained were well below the threshold that is required for the proposal of a novel species. The G+C content of the genomic DNA was 51.0 mol%. The phenotypic and genotypic data showed that strain AS/ASP6 (II) merits recognition as a representative of a novel species of the genus , for which the name sp. nov. is proposed; the type strain is AS/ASP6 (II) ( = MTCC 8491 = DSM 23997).

Funding
This study was supported by the:
  • , CSIR, Government of India , (Award NWP-006)
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2012-04-01
2020-08-05
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