1887

Abstract

A Gram-positive, spore-forming, aerobic actinomycete, strain Gsoil 519, was isolated from soil of a ginseng field of Pocheon province in South Korea. The closest phylogenetic relatives were Sp080513GE-26 (97.94 % 16S rRNA gene sequence similarity), NRRL B-24327 (97.84 %), subsp. DSM 40313 (97.84 %), NBRC 13015 (97.81 %), NBRC 15415 (97.81 %), NBRC 13078 (97.81 %), YIM 80305 (97.77 %) and NBRC 13041 (97.67 %). The G+C content of the genomic DNA was 71.8 mol%. The chemotaxonomic data [MK-9(H) and MK-9(H) as the major menaquinones; -diaminopimelic acid as a component of the cell-wall peptidoglycan; ribose, xylose, mannose and glucose as the major cell-wall sugars; and anteiso-C, iso-C, iso-C, anteiso-C and C as the major fatty acids] supported the affiliation of strain Gsoil 519 to the genus . The physiological and biochemical characteristics and the low level of DNA–DNA relatedness differentiated the isolate genotypically and phenotypically from recognized members of the genus . The isolate, therefore, represents a novel species, for which the name sp. nov. is proposed, with Gsoil 519 ( = KCTC 19139 = DSM 41871) as the type strain.

Funding
This study was supported by the:
  • 21C Frontier Microbial Genomics and Application Center Program, Ministry of Science & Technology (Award MG08-0101-2-0)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.029942-0
2012-04-01
2021-10-26
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/4/780.html?itemId=/content/journal/ijsem/10.1099/ijs.0.029942-0&mimeType=html&fmt=ahah

References

  1. Atlas R. M. 1993 Handbook of Microbiological Media Edited by Parks L. C. Boca Raton, FL: CRC Press;
    [Google Scholar]
  2. Buck J. D. 1982; Nonstaining (KOH) method for determination of gram reactions of marine bacteria. Appl Environ Microbiol 44:992–993[PubMed]
    [Google Scholar]
  3. Cui Y.-S., Lee S.-T., Im W.-T. 2009; Nocardioides ginsengisoli sp. nov., isolated from soil of a ginseng field. Int J Syst Evol Microbiol 59:3045–3050 [View Article][PubMed]
    [Google Scholar]
  4. Euzéby J. P. 1997; List of bacterial names with standing in nomenclature: a folder available on the Internet. Int J Syst Bacteriol 47:590–592 http://www.bacterio.cict.fr/ [View Article][PubMed]
    [Google Scholar]
  5. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [View Article]
    [Google Scholar]
  6. Felsenstein J. 1985; Confidence limit on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
    [Google Scholar]
  7. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [View Article]
    [Google Scholar]
  8. Hall T. A. 1999; BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98
    [Google Scholar]
  9. Hiraishi A., Ueda Y., Ishihara J., Mori T. 1996; Comparative lipoquinone analysis of influent sewage and activated sludge by high-performance liquid chromatography and photodiode array detection. J Gen Appl Microbiol 42:457–469 [View Article]
    [Google Scholar]
  10. Im W.-T., Kim S.-Y., Liu Q.-M., Yang J.-E., Lee S.-T., Yi T.-H. 2010; Nocardioides ginsengisegetis sp. nov., isolated from soil of a ginseng field. J Microbiol 48:623–628 [View Article][PubMed]
    [Google Scholar]
  11. Khan S. T., Tamura T., Takagi M., Shin-Ya K. 2010; Streptomyces tateyamensis sp. nov., Streptomyces marinus sp. nov. and Streptomyces haliclonae sp. nov., isolated from the marine sponge Haliclona sp.. Int J Syst Evol Microbiol 60:2775–2779[PubMed] [CrossRef]
    [Google Scholar]
  12. Kämpfer P., Kroppenstedt R. M., Dott W. 1991; A numerical classification of the genera Streptomyces and Streptoverticillium using miniaturized physiological tests. J Gen Microbiol 137:1831–1891 [CrossRef]
    [Google Scholar]
  13. Kimura M. 1983 The Neutral Theory of Molecular Evolution Cambridge: Cambridge University Press; [CrossRef]
    [Google Scholar]
  14. Komagata K., Suzuki K. 1987; Lipids and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207 [View Article]
    [Google Scholar]
  15. Korn-Wendisch F., Kutzner H. J. 1992; The family Streptomycetaceae . In The Prokaryotes, 2nd edn. vol. 1 pp. 921–995 Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H. New York: Springer;
    [Google Scholar]
  16. Kouker G., Jaeger K.-E. 1987; Specific and sensitive plate assay for bacterial lipases. Appl Environ Microbiol 53:211–213[PubMed]
    [Google Scholar]
  17. Kumar S., Nei M., Dudley J., Tamura K. 2008; mega: a biologist-centric software for evolutionary analysis of DNA and protein sequences. Brief Bioinform 9:299–306 [View Article][PubMed]
    [Google Scholar]
  18. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
    [Google Scholar]
  19. Minnikin D. E., Patel P. V., Alshamaony L., Goodfellow M. 1977; Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 27:104–117 [View Article]
    [Google Scholar]
  20. Moore D. D., Dowhan D. 1995; Preparation and analysis of DNA. In Current Protocols in Molecular Biology pp. 2–11 Edited by Ausubel F. W., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. New York: Wiley;
    [Google Scholar]
  21. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  22. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc;
  23. Staneck J. L., Roberts G. D. 1974; Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28:226–231[PubMed]
    [Google Scholar]
  24. Ten L. N., Im W.-T., Kim M.-K., Kang M.-S., Lee S.-T. 2004; Development of a plate technique for screening of polysaccharide-degrading microorganisms by using a mixture of insoluble chromogenic substrates. J Microbiol Methods 56:375–382 [View Article][PubMed]
    [Google Scholar]
  25. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
    [Google Scholar]
  26. Waksman S. A., Henrici A. T. 1943; The nomenclature and classification of the actinomycetes. J Bacteriol 46:337–341[PubMed]
    [Google Scholar]
  27. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [View Article]
    [Google Scholar]
  28. Williams S. T., Goodfellow M., Alderson G., Wellington E. M. H., Sneath P. H. A., Sackin M. J. 1983; Numerical classification of Streptomyces and related genera. J Gen Microbiol 129:1743–1813[PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.029942-0
Loading
/content/journal/ijsem/10.1099/ijs.0.029942-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error