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Abstract

A Gram-stain-positive, strictly aerobic, short-rod-shaped, non-motile strain (designated MJ32) was isolated from a sludge sample of the Daejeon sewage disposal plant in South Korea. A polyphasic approach was applied to study the taxonomic position of strain MJ32. Strain MJ32 showed highest 16S rRNA gene sequence similarity to DSM 44140 (98.1 %) and DSM 44015 (97.0 %); levels of sequence similarity to the type strains of other recognized species were less than 97.0 %. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain MJ32 belonged to the clade formed by members of the genus in the family . The G+C content of the genomic DNA of strain MJ32 was 69.2 mol%. Chemotaxonomically, strain MJ32 showed features typical of the genus . The predominant respiratory quinone was MK-9(H), the mycolic acids present had C–C carbon atoms, and the major fatty acids were C (34.6 %), tuberculostearic acid (21.8 %), Cω7 (19.5 %) and Cω9 (12.7 %). The peptidoglycan type was based on -2,6-diaminopimelic acid as the diagnostic diamino acid with glycolated sugars. On the basis of phylogenetic inference, fatty acid profile and other phenotypic properties, strain MJ32 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is MJ32 ( = KCTC 19771 = JCM 16923).

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2012-11-01
2020-01-22
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