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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 54, Issue 5

Comparison of 16S rRNA, , , , and genes within the family fam. nov. Free

Abstract

The sequences of five conserved genes, in addition to the 16S rRNA gene, were investigated in 30 members of the fam. nov. All members of the examined contained , suggesting that they are capable of nitrogen fixation, which may explain their ability to compete effectively in nitrogen-poor subsurface environments undergoing remediation for petroleum or metal contamination. The phylogenies predicted from , , , and were generally in agreement with the phylogeny predicted from 16S rRNA gene sequences. Furthermore, phylogenetic analysis of concatemers constructed from all five protein-coding genes corresponded closely with the 16S rRNA gene-based phylogeny. This study demonstrated that the is a phylogenetically coherent family within the -subclass of the that is composed of three distinct phylogenetic clusters: , and . The sequence data provided here will make it possible to discriminate better between physiologically distinct members of the , such as and species, in geobacteraceae-dominated microbial communities and greatly expands the potential to identify geobacteraceae sequences in libraries of environmental genomic DNA.

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2004-09-01
2025-05-21
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Comparison of 16S rRNA, nifD, recA, gyrB, rpoB and fusA genes within the family Geobacteraceae fam. nov.
Int J Syst Evol Microbiol 54, 1591 (2004); https://doi.org/10.1099/ijs.0.02958-0
/content/journal/ijsem/10.1099/ijs.0.02958-0
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