1887

Abstract

A group of pigmented, slowly growing mycobacteria identified by 16S rRNA gene sequencing as ‘MCRO 33’ (GenBank accession no. AF152559) have been isolated from several clinical specimens in various laboratories across Canada. Genotypically, the organism is most closely related to . However, it presents with a similar phenotypic profile to . Several reference strains obtained from ATCC and TMC culture collections, previously identified as or , have also been found to possess the MCRO 33 16S rRNA gene sequence. Biochemical testing, susceptibility testing, HPLC, gene and 16S–23S spacer (ITS1) sequencing were performed on clinical and reference strains to characterize further this unique species. Of the clinical strains, one was isolated from a cervix biopsy whereas all other clinical isolates were obtained from respiratory samples. In one patient, symptoms, imaging and repeat clinical specimens positive on culture for this organism were suggestive of active clinical disease. The description of this species, for which the name sp. nov. is proposed, follows the present trend of a large number of novel species identified due in great part to sequence-based methods. The type strain is HSC68 (=ATCC BAA-614=DSM 44648).

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2004-09-01
2021-10-28
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References

  1. Aikin K. R., Johnston R. F. 1978; Tuberculous-like disease produced by M. scrofulaceum . Pa Med 81:38–41
    [Google Scholar]
  2. Brown B. A., Wallace R. J. Jr, Onyi G. O. 1992; Activities of clarithromycin against eight slowly growing species of nontuberculous mycobacteria, determined by using a broth microdilution MIC system. Antimicrob Agents Chemother 36:1987–1990 [CrossRef]
    [Google Scholar]
  3. Brown-Elliott B. A., Crist C. J., Mann L. B., Wilson R. W., Wallace J. R. Jr 2003; In vitro activity of linezolid against slowly growing nontuberculous mycobacteria. Antimicrob Agents Chemother 47:1736–1738 [CrossRef]
    [Google Scholar]
  4. Butler W. R., Floyd M. M., Silcox V. A. & 9 other authors 1996 Standardized method for HPLC identification of mycobacteria Atlanta, GA: Centers for Disease Control and Prevention, US Department of Health and Human Services;
    [Google Scholar]
  5. Butler W. R., Floyd M. M., Silcox V. A. & 9 other authors 1999 Mycolic acid pattern standard for HPLC identification of mycobacteria Atlanta, GA: Centers for Disease Control and Prevention, US Department of Health and Human Services;
    [Google Scholar]
  6. Corbett E. L., Hay M., Churchyard G. J., Herselman P., Clayton T., Williams B. G., Hayes R., Mulder D., De-Cock K. M. 1999; Mycobacterium kansasii and M. scrofulaceum isolates from HIV-negative South African gold miners: incidence, clinical significance and radiology. Int J Tuberc Lung Dis 3:501–507
    [Google Scholar]
  7. da Silva R. A., Werneck Barreto A. M., Dias Campos C. E., Villas-Boas D. S., Fonseca L., Saad M. H., Degrave W. M., Suffys P. N. 2002; Novel allelic variants of mycobacteria isolated in Brazil as determined by PCR-restriction enzyme analysis of hsp65. J Clin Microbiol 40:4191–4196 [CrossRef]
    [Google Scholar]
  8. Dustin P., Demol P., Derks-Jacobovitz D., Cremer N., Vis H. 1980; Generalized fatal chronic infection by Mycobacterium scrofulaceum with severe amyloidosis in a child. Pathol Res Pract 168:237–248 [CrossRef]
    [Google Scholar]
  9. Frothingham R., Wilson K. H. 1993; Sequence-based differentiation of strains in the Mycobacterium avium complex. J Bacteriol 175:2818–2825
    [Google Scholar]
  10. Glickman S. E., Kilburn J. O., Butler W. R., Ramos L. S. 1994; Rapid identification of mycolic acid patterns of mycobacteria by high-performance liquid chromatography using pattern recognition software and a Mycobacterium library. J Clin Microbiol 32:740–745
    [Google Scholar]
  11. Goodfellow M., Magee J. G. 2001; Taxonomy of mycobacteria. In Mycobacteria I: Basic Aspects pp  1–71 Edited by Gangadharam P. R., Jenkin P. A. New York: International Thomson Publishing;
    [Google Scholar]
  12. Hall L., Doerr K. A., Wohlfiel S. L., Roberts G. D. 2003; Evaluation of the MicroSeq system for identification of mycobacteria by 16S ribosomal DNA sequencing and its integration into a routine clinical mycobacteriology laboratory. J Clin Microbiol 41:1447–1453 [CrossRef]
    [Google Scholar]
  13. Harmsen D., Rothganger J., Frosch M., Albert J. 2002; RIDOM: ribosomal differentiation of medical micro-organisms database. Nucleic Acids Res 30:416–417 [CrossRef]
    [Google Scholar]
  14. Heifets L., Lindholm-Levy P., Libonati J. P., Hooper N., Laszlo A., Cynamon M., Siddiqi S. 1993 Radiometric broth macrodilution method for the determination of minimal inhibitory concentrations (MIC) with Mycobacterium avium complex isolates Denver, CO: National Jewish Center for Immunology and Respiratory Medicine;
    [Google Scholar]
  15. Holberg-Petersen M., Steinbakk M., Figenschau K. J., Jantzen E., Eng J., Melby K. K. 1999; Identification of clinical isolates of Mycobacterium spp. by sequence analysis of the 16S ribosomal RNA gene. Experience from a clinical laboratory. APMIS 107:231–239 [CrossRef]
    [Google Scholar]
  16. Hsueh P. R., Hsiue T. R., Jarn J. J., Ho S. W., Hsieh W. C. 1996; Disseminated infection due to Mycobacterium scrofulaceum in an immunocompetent host. Clin Infect Dis 22:159–161 [CrossRef]
    [Google Scholar]
  17. Joshi W., Davidson P. M., Jones P. G., Campbell P. E., Roberton D. M. 1989; Non-tuberculous mycobacterial lymphadenitis in children. Eur J Pediatr 148:751–754 [CrossRef]
    [Google Scholar]
  18. Kent P. T., Kubica G. P. 1985 Public Health Mycobacteriology: a Guide for the Level III Laboratory Atlanta, GA: Department of Health and Human Services, Centers for Disease Control;
    [Google Scholar]
  19. Metchock B., Nolte F. S., Wallace R. J. Jr 1999; Mycobacterium . In Manual of Clinical Microbiology pp  399–437 Edited by Murray P. R., Baron E. J., Pfaller M. A., Tenover F. C., Yolken R. H. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  20. Murray-Leisure K. A., Egan N., Weitekamp M. R. 1987; Skin lesions caused by Mycobacterium scrofulaceum . Arch Dermatol 123:369–370 [CrossRef]
    [Google Scholar]
  21. NCCLS 2003 Susceptibility testing of mycobacteria, nocardiae, and other aerobic actinomycetes . Approved Standard M24-A Wayne, PA: National Committee for Clinical Laboratory Standards;
    [Google Scholar]
  22. Pauls R. J., Turenne C. Y., Wolfe J. N., Kabani A. 2003; A high proportion of novel mycobacteria species identified by 16S rDNA analysis among slowly growing Accuprobe negative strains in a clinical setting. Am J Clin Pathol 120:560–566 [CrossRef]
    [Google Scholar]
  23. Prissick F. H., Masson A. M. 1956; Cervical lymphadenitis in children caused by chromogenic mycobacteria. J Can Med Assoc 75:798–803
    [Google Scholar]
  24. Rogall T., Wolters J., Flohr T., Bottger E. C. 1990; Towards a phylogeny and definition of species at the molecular level within the genus Mycobacterium . Int J Syst Bacteriol 40:323–330 [CrossRef]
    [Google Scholar]
  25. Roth A., Reischl U., Streubel A., Naumann L., Kroppenstedt R. M., Habicht M., Fischer M., Mauch H. 2000; Novel diagnostic algorithm for identification of mycobacteria using genus-specific amplification of the 16S–23S rRNA gene spacer and restriction endonucleases. J Clin Microbiol 38:1094–1104
    [Google Scholar]
  26. Saito H., Tomioka H., Sato K., Tasaka H., Dawson D. J. 1990; Identification of various serovar strains of Mycobacterium avium complex by using DNA probes specific for Mycobacterium avium and Mycobacterium intracellulare . J Clin Microbiol 28:1694–1697
    [Google Scholar]
  27. Sanders J. W., Walsh A. D., Snider R. L., Sahn E. E. 1995; Disseminated Mycobacterium scrofulaceum infection: a potentially treatable complication of AIDS. Clin Infect Dis 20:549–556 [CrossRef]
    [Google Scholar]
  28. Springer B., Stockman L., Teschner K., Roberts G. D., Bottger E. C. 1996; Two-laboratory collaborative study on identification of mycobacteria: molecular versus phenotypic methods. J Clin Microbiol 34:296–303
    [Google Scholar]
  29. Swanson D. S., Pan X., Musser J. M. 1996; Identification and subspecific differentiation of Mycobacterium scrofulaceum by automated sequencing of a region of the gene (hsp65) encoding a 65-kilodalton heat shock protein. J Clin Microbiol 34:3151–3159
    [Google Scholar]
  30. Telenti A., Marchesi F., Balz M., Bally F., Bottger E. C., Bodmer T. 1993; Rapid identification of mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis. J Clin Microbiol 31:175–178
    [Google Scholar]
  31. Tomioka H., Saito H., Sato K., Tasaka H., Dawson D. J. 1993; Identification of Mycobacterium avium complex strains belonging to serovars 21–28 by three commercial DNA probe tests. Tuber Lung Dis 74:91–95 [CrossRef]
    [Google Scholar]
  32. Tortoli E. 2003; Impact of genotypic studies on mycobacterial taxonomy: the new mycobacteria of the 1990s. Clin Microbiol Rev 16:319–354 [CrossRef]
    [Google Scholar]
  33. Tortoli E., Bartoloni A., Bottger E. C. 8 other authors 2001; Burden of unidentifiable mycobacteria in a reference laboratory. J Clin Microbiol 39:4058–4065 [CrossRef]
    [Google Scholar]
  34. Turenne C. Y., Tschetter L., Wolfe J., Kabani A. 2001; Necessity of quality-controlled 16S rRNA gene sequence databases: identifying nontuberculous Mycobacterium species. J Clin Microbiol 39:3637–3648 [CrossRef]
    [Google Scholar]
  35. Turenne C., Chedore P., Wolfe J., Jamieson F., Broukhanski G., May K., Kabani A. 2002; Mycobacterium lacus sp. nov., a novel slowly growing, non-chromogenic clinical isolate. Int J Syst Evol Microbiol 52:2135–2140 [CrossRef]
    [Google Scholar]
  36. Wallace R. J. Jr, Nash D. R., Steele L. C., Steingrube V. 1986; Susceptibility testing of slowly growing mycobacteria by a microdilution MIC method with 7H9 broth. J Clin Microbiol 24:976–981
    [Google Scholar]
  37. Wolinsky E. 1979; Nontuberculous mycobacteria and associated diseases. Am Rev Respir Dis 119:107–159
    [Google Scholar]
  38. Wolinsky E. 1995; Mycobacterial lymphadenitis in children: a prospective study of 105 nontuberculous cases with long-term follow-up. Clin Infect Dis 20:954–963 [CrossRef]
    [Google Scholar]
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