1887

Abstract

A group of pigmented, slowly growing mycobacteria identified by 16S rRNA gene sequencing as ‘MCRO 33’ (GenBank accession no. AF152559) have been isolated from several clinical specimens in various laboratories across Canada. Genotypically, the organism is most closely related to . However, it presents with a similar phenotypic profile to . Several reference strains obtained from ATCC and TMC culture collections, previously identified as or , have also been found to possess the MCRO 33 16S rRNA gene sequence. Biochemical testing, susceptibility testing, HPLC, gene and 16S–23S spacer (ITS1) sequencing were performed on clinical and reference strains to characterize further this unique species. Of the clinical strains, one was isolated from a cervix biopsy whereas all other clinical isolates were obtained from respiratory samples. In one patient, symptoms, imaging and repeat clinical specimens positive on culture for this organism were suggestive of active clinical disease. The description of this species, for which the name sp. nov. is proposed, follows the present trend of a large number of novel species identified due in great part to sequence-based methods. The type strain is HSC68 (=ATCC BAA-614=DSM 44648).

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2004-09-01
2019-08-19
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vol. , part 5, pp. 1543 - 1551

Alignment of the 16S-23S rRNA spacer (ITS1) sequences. SqvI-SqvIV, sp. nov. sequevars; Mscrof, ATCC 19981 (determined in our laboratory). Nucleotides that differ from are shown. The length (in nt) of the ITS1 fragment from position 1 is indicated.

Alignment of a hypervariable region of the gene. Numbering of Mtb represents that of the complete gene using the sequence Erdman 65 kDa (M15467). All other sequences were determined in our laboratory. Numbering of all other sequences represents the base position on the amplified 441 bp fragment. SqvI-SqvV, sp. nov. sequevars; Msc, ATCC 19981 ; Mav, ATCC 25291 ; Min, ATCC 13950 .

16S rRNA gene sequence analysis of sp. nov. strain HSC-68 against its genotypically closest relatives.

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