1887

Abstract

A Gram-staining-negative, aerobic, non-motile and rod-shaped bacterial strain, designated DPG-21, was isolated from seawater from the South Sea in Korea, and investigated using a polyphasic taxonomic approach. Strain DPG-21 grew optimally at pH 7.0–8.0, at 30 °C and in the presence of 2 % (w/v) NaCl. In a neighbour-joining phylogenetic tree based on 16S rRNA gene sequences, strain DPG-21 clustered with B51 (with a sequence similarity of 97.1 %); the novel strain showed lower 16S rRNA gene sequence similarities (<95.4 %) with the other species included in the tree. The mean DNA–DNA relatedness value between strain DPG-21 and . DSM 19548 was 12 %. The predominant ubiquinones of strain DPG-21 were Q-10 and Q-9 while Cω7 was the strain’s major fatty acid. The polar lipid profile of strain DPG-21 was similar to that of DSM 19548. The genomic DNA G+C content of the novel strain was 69.6 mol%. Some phenotypic properties and the phylogenetic and genetic data indicated that strain DPG-21 was distinct from . and represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is DPG-21 ( = KCTC 23424 = CCUG 60524).

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2012-03-01
2020-01-21
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References

  1. Bruns A., Rohde M., Berthe-Corti L.. ( 2001;). Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. . Int J Syst Evol Microbiol 51:, 1997–2006. [CrossRef][PubMed]
    [Google Scholar]
  2. Cowan S. T., Steel K. J.. ( 1965;). Manual for the Identification of Medical Bacteria. London:: Cambridge University Press;.
    [Google Scholar]
  3. Ezaki T., Hashimoto Y., Yabuuchi E.. ( 1989;). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39:, 224–229. [CrossRef]
    [Google Scholar]
  4. Harwati T. U., Kasai Y., Kodama Y., Susilaningsih D., Watanabe K.. ( 2009;). Tropicimonas isoalkanivorans gen. nov., sp. nov., a branched-alkane-degrading bacterium isolated from Semarang Port in Indonesia. . Int J Syst Evol Microbiol 59:, 388–391. [CrossRef][PubMed]
    [Google Scholar]
  5. Komagata K., Suzuki K.. ( 1987;). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19:, 161–207. [CrossRef]
    [Google Scholar]
  6. Lányí B.. ( 1987;). Classical and rapid identification methods for medically important bacteria. . Methods Microbiol 19:, 1–67. [CrossRef]
    [Google Scholar]
  7. Leifson E.. ( 1963;). Determination of carbohydrate metabolism of marine bacteria. . J Bacteriol 85:, 1183–1184.[PubMed]
    [Google Scholar]
  8. Martens T., Heidorn T., Pukall R., Simon M., Tindall B. J., Brinkhoff T.. ( 2006;). Reclassification of Roseobacter gallaeciensis Ruiz-Ponte et al. 1998 as Phaeobacter gallaeciensis gen. nov., comb. nov., description of Phaeobacter inhibens sp. nov., reclassification of Ruegeria algicola (Lafay et al. 1995) Uchino et al. 1999 as Marinovum algicola gen. nov., comb. nov., and emended descriptions of the genera Roseobacter, Ruegeria and Leisingera. . Int J Syst Evol Microbiol 56:, 1293–1304. [CrossRef][PubMed]
    [Google Scholar]
  9. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H.. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  10. Sasser M.. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc.;
  11. Stackebrandt E., Goebel B. M.. ( 1994;). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44:, 846–849. [CrossRef]
    [Google Scholar]
  12. Tamaoka J., Komagata K.. ( 1984;). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25:, 125–128. [CrossRef]
    [Google Scholar]
  13. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E.. & other authors ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
  14. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H.. ( 1996;). Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. . Int J Syst Bacteriol 46:, 502–505. [CrossRef]
    [Google Scholar]
  15. Yoon J.-H., Lee S. T., Park Y.-H.. ( 1998;). Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. . Int J Syst Bacteriol 48:, 187–194. [CrossRef][PubMed]
    [Google Scholar]
  16. Yoon J.-H., Kang K. H., Park Y.-H.. ( 2003;). Psychrobacter jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. . Int J Syst Evol Microbiol 53:, 449–454. [CrossRef][PubMed]
    [Google Scholar]
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