1887

Abstract

Three enterococcal isolates, CCRI-16620, CCRI-16986 and CCRI-16985, originating from water were characterized using morphological, biochemical and molecular taxonomic methods. 16S rRNA gene sequence analysis classified all three strains in the species group. The phylogenetic tree of 16S rRNA gene sequences showed that the three isolates form two separate branches. The first branch is represented by strains CCRI-16620 and CCRI-16986 and the second branch by strain CCRI-16985. Further sequence analysis of the housekeeping genes (encoding RNA polymerase α subunit), (phenylalanyl-tRNA synthase), (elongation factor Tu) and (ATP synthase β-subunit) as well as the results of amplified fragment length polymorphism (AFLP) DNA fingerprinting and DNA–DNA hybridization experiments confirmed the distinct status of these strains. Moreover, biochemical tests allowed phenotypic differentiation of the strains from the other species of the species group. On the basis of the results obtained, the names sp. nov. (type strain CCRI-16986 = CCUG 59304 = DSM 23328 = LMG 26304) and sp. nov. (type strain CCRI-16985 = CCUG 59306 = DSM 23327 = LMG 26306) are proposed for the two hitherto undescribed species.

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2012-06-01
2019-10-20
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