1887

Abstract

Data on 72 non-pigmented bacterial strains that specifically induce nitrogen-fixing root nodules on the legume species , and are reviewed. By SDS-PAGE analysis of total protein patterns and by 16S rRNA PCR-RFLP, these strains form a homogeneous group that is separate from other legume root-nodule-forming bacteria. The 16S rRNA gene-based phylogeny indicates that these bacteria belong to the genus . They can grow on C compounds such as methanol, formate and formaldehyde but not methylamine as sole carbon source, and carry an gene, encoding methanol dehydrogenase, which supports their methylotrophic metabolism. Presence of a nodulation gene, and ability to nodulate plants of species and to fix nitrogen are features that separate the strains currently included in this group from other members of the genus . The present study includes additional genotypic and phenotypic characterization of this novel species, i.e. gene sequence, morphology, physiology, enzymic and carbon source assimilation tests and antibiotic resistance. The name sp. nov. (type strain, ORS 2060=CNCM I 2342=LMG 21967) is proposed for this group of root-nodule-forming bacteria.

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2004-11-01
2019-10-18
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References

  1. Corpet, F. ( 1988; ). Multiple sequence alignment with hierarchical clustering. Nucleic Acids Res 16, 10881–10890.[CrossRef]
    [Google Scholar]
  2. Doronina, N. V., Trotsenko, Y. A., Kuznetsov, B. B., Tourova, T. P. & Salkinoja-Salonen, M. S. ( 2002; ). Methylobacterium suomiense sp. nov. and Methylobacterium lusitanum sp. nov., aerobic, pink-pigmented, facultatively methylotrophic bacteria. Int J Syst Evol Microbiol 52, 773–776.[CrossRef]
    [Google Scholar]
  3. Dreyfus, B., Giraud, E. & Boivin-Masson, C. ( 1999; ). Nouvelles bactéries symbiotiques et leurs applications. Brevet d'Invention no. 99 14179. Institut National de la Propriété Industrielle, Paris, France. (http://www.inpi.fr/brevet/pdf/COSMOS1/CFR02800/02800747A1.pdf). Patent WO 0134777-A 3, 17 May 2001, “Symbiotic bacteria and their uses”.
  4. Green, P. ( 1992; ). The genus Methylobacterium. In The Prokaryotes, 2nd edn, pp. 2342–2349. Edited by A. Balows, H. G. Trüper, M. Dworkin, W. Harder & K.-H. Schleifer. New York: Springer-Verlag.
  5. Holland, M. A. ( 1997; ). Methylobacterium and plants. Recent Res Dev Plant Physiol 1, 207–212.
    [Google Scholar]
  6. Jaftha, J. B., Strijdom, B. W. & Steyn, P. L. ( 2002; ). Characterization of pigmented methylotrophic bacteria which nodulate Lotononis bainesii. Syst Appl Microbiol 25, 440–449.[CrossRef]
    [Google Scholar]
  7. Kersters, K., Hinz, K. H., Hertle, H., Segers, P., Lievens, A., Siegmann, O. & De Ley, J. ( 1984; ). Bordetella avium sp. nov., isolated from the respiratory tracts of turkeys and other birds. Int J Syst Bacteriol 34, 56–70.[CrossRef]
    [Google Scholar]
  8. Martinez Romero, E. ( 1994; ). Recent developments in Rhizobium taxonomy. Plant Soil 161, 11–20.[CrossRef]
    [Google Scholar]
  9. Patt, T. E., Cole, G. C. & Hanson, R. S. ( 1976; ). Methylobacterium, a new genus of facultatively methylotrophic bacteria. Int J Syst Bacteriol 26, 226–229.[CrossRef]
    [Google Scholar]
  10. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  11. Samba, R. T., De Lajudie, P., Gillis, M., Neyra, M., Spencer-Barreto, M. M. & Dreyfus, B. ( 1999; ). Diversity of rhizobia nodulating Crotalaria spp. from Senegal. Symbiosis 27, 259–268.
    [Google Scholar]
  12. Swofford, D. ( 1998; ). paup*: phylogenetic analysis using parsimony (*and other methods), version 4. Sunderland, MA: Sinauer Associates.
  13. Sy, A., Giraud, E., Samba, R., de Lajudie, P., Gillis, M. & Dreyfus, B. ( 2001a; ). Certaines légumineuses du genre Crotalaria sont spécifiquement nodulées par une nouvelle espèce de Methylobacterium. Can J Microbiol 47, 503–508.[CrossRef]
    [Google Scholar]
  14. Sy, A., Giraud, E., Jourand, P. & 8 other authors ( 2001b; ). Methylotrophic Methylobacterium bacteria nodulate and fix nitrogen in symbiosis with legumes. J Bacteriol 183, 214–220.[CrossRef]
    [Google Scholar]
  15. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  16. Van Aken, B., Peres, C. M., Lafferty Doty, S., Yoon, J. M. & Schnoor, J. L. ( 2004; ). Methylobacterium populi sp. nov., a novel aerobic, pink-pigmented, facultatively methylotrophic, methane-utilizing bacterium isolated from poplar trees (Populus deltoides×nigra DN34). Int J Syst Evol Microbiol 54, 1191–1196.[CrossRef]
    [Google Scholar]
  17. van Rhijn, P. & Vanderleyden, J. ( 1995; ). The Rhizobium–plant symbiosis. Microbiol Rev 59, 124–142.
    [Google Scholar]
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