1887

Abstract

A rod-shaped, yellow and strictly aerobic marine bacterium, designated KYW382, was isolated from seawater collected from the South Sea, Republic of Korea. Cells were Gram-negative and catalase- and oxidase-positive. The major fatty acids were iso-C G, iso-C, iso-C 3-OH, iso-C 3-OH and anteiso-C. The DNA G+C content was 32.4 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain KYW382 constituted an evolutionary lineage within the radiation enclosing the members of the genus . The closest neighbour was SMK-12 (96.1 % 16S rRNA gene sequence similarity). A number of phenotypic characteristics distinguished strain KYW382 from the described members of the genus . On the basis of the data presented in this study, strain KYW382 represents a novel species, for which the name sp. nov. is proposed. The type strain is KYW382 ( = KCTC 23303  = JCM 17455). An emended description of the genus is also given.

Funding
This study was supported by the:
  • , 21C Frontier Microbial Genomics and Applications Center Program, Ministry of Education, Science & Technology, Republic of Korea
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.028944-0
2012-01-01
2020-07-13
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/1/150.html?itemId=/content/journal/ijsem/10.1099/ijs.0.028944-0&mimeType=html&fmt=ahah

References

  1. Barrow G. I., Feltham R. K. A. 1993 Cowan and Steel's Manual for the Identification of Medical Bacteria, 3rd edn. Cambridge: Cambridge University Press; [CrossRef]
    [Google Scholar]
  2. Bernardet J.-F., Nakagawa Y. 2006; An introduction to the family Flavobacteriaceae . In The Prokaryotes, a Handbook on the Biology of Bacteria, 3rd edn. vol. 7 pp. 455–480 Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E. New York: Springer;
    [Google Scholar]
  3. Bernardet J. F., Nakagawa Y., Holmes B. 2002; Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070 [CrossRef][PubMed]
    [Google Scholar]
  4. Chun J., Goodfellow M. 1995; A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. Int J Syst Bacteriol 45:240–245 [CrossRef][PubMed]
    [Google Scholar]
  5. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y.-W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [CrossRef][PubMed]
    [Google Scholar]
  6. CLSI 2003; Performance standards for antimicrobial disk susceptibility tests, 8th edn.. Wayne, PA: Clinical and Laboratory Standards Institute;
  7. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [CrossRef][PubMed]
    [Google Scholar]
  8. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  9. Felsenstein J. 1993; phylip (phylogeny inference package) version 3.5c. Distributed by the author.. Department of Genome Sciences, University of Washington, Seattle, USA.
  10. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [CrossRef]
    [Google Scholar]
  11. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism vol. 3 pp. 21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  12. Jung S.-Y., Kang S.-J., Lee M.-H., Lee S.-Y., Oh T.-K., Yoon J.-H. 2005; Gaetbulibacter saemankumensis gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from a tidal flat sediment in Korea. Int J Syst Evol Microbiol 55:1845–1849 [CrossRef][PubMed]
    [Google Scholar]
  13. Kovács N. 1956; Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature 178:703 [CrossRef][PubMed]
    [Google Scholar]
  14. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [CrossRef][PubMed]
    [Google Scholar]
  15. McCammon S. A., Bowman J. P. 2000; Taxonomy of Antarctic Flavobacterium species: description of Flavobacterium gillisiae sp. nov., Flavobacterium tegetincola sp. nov., and Flavobacterium xanthum sp. nov., nom. rev. and reclassification of [Flavobacterium] salegens as Salegentibacter salegens gen. nov., comb. nov.. Int J Syst Evol Microbiol 50:1055–1063 [CrossRef][PubMed]
    [Google Scholar]
  16. MIDI 1999 Sherlock Microbial Identification System Operating Manual, version 3.0 Newark, DE: MIDI, Inc;
    [Google Scholar]
  17. Reichenbach H. 1989; The order Cytophagales Leadbetter 1974, 99AL . In Bergey’s Manual of Systematic Bacteriology vol. 3 pp. 2011–2013 Edited by Staley J. T., Bryant M. P., Pfennig N., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  18. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  19. Skerman V. B. D. 1967 A Guide to the Identification of the Genera of Bacteria, 2nd edn. Baltimore: Williams & Wilkins;
    [Google Scholar]
  20. Smibert R. M., Krieg N. R. 1994; General characterization. In Methods for General and Molecular Bacteriology pp. 607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  21. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  22. Swofford D. L. 1998; Phylogenetic analysis using parsimony (paup), version 4.. Sunderland, MA: Sinauer Associates;
  23. Teather R. M., Wood P. J. 1982; Use of Congo red-polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen. Appl Environ Microbiol 43:777–780[PubMed]
    [Google Scholar]
  24. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef][PubMed]
    [Google Scholar]
  25. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. & other authors ( 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  26. Yamaguchi S., Yokoe M. 2000; A novel protein-deamidating enzyme from Chryseobacterium proteolyticum sp. nov., a newly isolated bacterium from soil. Appl Environ Microbiol 66:3337–3343 [CrossRef][PubMed]
    [Google Scholar]
  27. Yang S.-J., Cho J.-C. 2008; Gaetbulibacter marinus sp. nov., isolated from coastal seawater, and emended description of the genus Gaetbulibacter . Int J Syst Evol Microbiol 58:315–318 [CrossRef][PubMed]
    [Google Scholar]
  28. Yang S.-H., Kwon K. K., Lee H.-S., Kim S.-J. 2006; Shewanella spongiae sp. nov., isolated from a marine sponge. Int J Syst Evol Microbiol 56:2879–2882 [CrossRef][PubMed]
    [Google Scholar]
  29. Yumoto I., Hirota K., Yamaga S., Nodasaka Y., Kawasaki T., Matsuyama H., Nakajima K. 2004; Bacillus asahii sp. nov., a novel bacterium isolated from soil with the ability to deodorize the bad smell generated from short-chain fatty acids. Int J Syst Evol Microbiol 54:1997–2001 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.028944-0
Loading
/content/journal/ijsem/10.1099/ijs.0.028944-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error